Bacillus altitudinis HNH7 and Bacillus velezensis HNH9 promote plant growth through upregulation of growth-promoting genes in upland cotton

Hasan, Nadeem; Khan, Irfan Ullah; Farzand, Ayaz; Zhou, Heng; Moosa, Anam; Saleem, Muhammad Hamzah; Tang, Canming

doi:10.1111/jam.15511

Cited by 23 publications

(25 citation statements)

References 58 publications

(81 reference statements)

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“…LC–MS successfully detected the presence of surfactin, iturin, fengycin, bacillaene, bacillibactin, plantazolicin, and bacilysin in CHGP12. In a similar report LPs produced by B. altitudinis HNH7 and B. velezensis HNH9 were detected through LC–MS, and it was concluded that the significantly high inhibitory potential of these strains was due to the presence of LPs 20 . According to previous reports, the simultaneous detection and production of antimicrobial LPs and their antifungal activities are correlated 46 .…”

Section: Discussionmentioning

confidence: 60%

“…In addition to hydrolytic enzymes, CHGP12 also showed the potential to produce siderophores, IAA and GA. In previous reports Bacillus species have been reported to produce hydrolytic enzymes, siderophores, IAA, and GA 20,52 . Several previous reports have proved the direct effect of siderophores on the growth promotion of plants 20,53,54 .…”

Section: Discussionmentioning

confidence: 96%

“…Bacillus species produce hydrolytic enzymes such as proteases, lipases, amylases, and cellulases which are important industrial and commercial enzymes 18,19 . These hydrolytic enzymes contribute to the antifungal activity of Bacillus species by causing disintegration and lysis of fungal cell walls 20 . Bacillus species produce a variety of biologically active metabolites that not only play a role in the maintenance of their metabolism but also promote the growth of plants 21 .…”

Section: Introductionmentioning

confidence: 99%

“…IAA and GA are two important plant growth regulators that can induce cell division, elongation, and differentiation 23 . Based on previous reports a strong contribution of IAA and GA to plant growth promotion has been reported 20,24 . However, there is limited evidence of the involvement of Bacillus species in plant growth promotion under field conditions.…”

Section: Introductionmentioning

confidence: 99%

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Bacillus thuringiensisCHGP12 uses a multifaceted approach for the suppression of Fusarium oxysporum f. sp. ciceris and to enhance the biomass of chickpea plants

Fatima

Mahmood

Moosa

et al. 2022

Pest Management Science

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BACKGROUND Bacillus species synthesize antifungal lipopeptides (LPs) making them a sustainable and eco‐friendly management option to combat Fusarium wilt of chickpea. RESULTS In this study, 18 endophytic Bacillus strains were assessed for their antifungal activity against Fusarium oxysporum f. sp. ciceris (FOC) associated with Fusarium wilt of chickpea. Among them, 13 strains produced significant inhibition zones in a direct antifungal assay while five strains failed to produce the inhibition of FOC. Bacillus thuringiensis CHGP12 exhibited the highest inhibition 3.45 cm of FOC. The LPs extracted from CHGP12 showed significant inhibition of the pathogen. Liquid chromatography–mass spectrometry (LC–MS) analysis confirmed that CHGP12 possessed the ability to produce fengycin, surfactin, iturin, bacillaene, bacillibactin, plantazolicin, and bacilysin. In an in vitro qualitative assay CHGP12 exhibited the ability to produce lipase, amylase, cellulase, protease, siderophores, and indole 3‐acetic acid (IAA). IAA and gibberellic acid (GA) were quantified using ultra‐performance liquid chromatography (UPLC) with 370 and 770 ng mL−1 concentrations of IAA and GA respectively. Furthermore, the disease severity showed a 40% decrease over control in CHGP12 treated plants compared to the control in a glasshouse experiment. Moreover, CHGP12 also exhibited a significant increase in total biomass of the plants namely, root and shoot growth parameters, stomatal conductance, and photosynthesis rate. CONCLUSION In conclusion, our findings suggest that B. thuringiensis CHGP12 is a promising strain with high antagonistic and growth‐promoting potential against Fusarium wilt of chickpea. © 2022 Society of Chemical Industry.

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bacillus thuringiensisCHGP12 uses a multifaceted approach for the suppression of Fusarium oxysporum f. sp. ciceris and to enhance the biomass of chickpea plants

Fatima

Mahmood

Moosa

et al. 2022

Pest Management Science

Self Cite

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“…and Streptomyces spp. ( Kataoka et al, 2021 ), Rhizobium pusense MB-17a ( Chaudhary et al, 2021 ), P. liquidambaris ( Zhou et al, 2022 ), Bacillus altitudinis ( Zhang et al, 2021 ; Hasan et al, 2022 ), and P. giganteum ( Yankey et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Deciphering the potential of a plant growth promoting endophyte Rhizobium sp. WYJ-E13, and functional annotation of the genes involved in the metabolic pathway

Huang

Zeng²,

Chen³

et al. 2022

Front. Microbiol.

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Plant growth-promoting rhizobacteria (PGPR) are well-acknowledged root endophytic bacteria used for plant growth promotion. However, which metabolites produced by PGPR could promote plant growth remains unclear. Additionally, which genes are responsible for plant growth-promoting traits is also not elucidated. Thus, as comprehensive understanding of the mechanism of endophyte in growth promotion is limited, this study aimed to determine the metabolites and genes involved in plant growth-promotion. We isolated an endophytic Rhizobium sp. WYJ-E13 strain from the roots of Curcuma wenyujin Y.H. Chen et C. Ling, a perennial herb and medicinal plant. The tissue culture experiment showed its plant growth-promoting ability. The bacterium colonization in the root was confirmed by scanning electron microscopy and paraffin sectioning. Furthermore, it was noted that the WYJ-E13 strain produced cytokinin, anthranilic acid, and L-phenylalanine by metabolome analysis. Whole-genome analysis of the strain showed that it consists of a circular chromosome of 4,350,227 bp with an overall GC content of 60.34%, of a 2,149,667 bp plasmid1 with 59.86% GC, and of a 406,180 bp plasmid2 with 58.05% GC. Genome annotation identified 4,349 putative protein-coding genes, 51 tRNAs, and 9 rRNAs. The CDSs number allocated to the Kyoto Encyclopedia of Genes and Genomes, Gene Ontology, and Clusters of Orthologous Genes databases were 2027, 3,175 and 3,849, respectively. Comparative genome analysis displayed that Rhizobium sp. WYJ-E13 possesses the collinear region among three species: Rhizobium acidisoli FH23, Rhizobium gallicum R602 and Rhizobium phaseoli R650. We recognized a total set of genes that are possibly related to plant growth promotion, including genes involved in nitrogen metabolism (nifU, gltA, gltB, gltD, glnA, glnD), hormone production (trp ABCDEFS), sulfur metabolism (cysD, cysE, cysK, cysN), phosphate metabolism (pstA, pstC, phoB, phoH, phoU), and root colonization. Collectively, these findings revealed the roles of WYJ-E13 strain in plant growth-promotion. To the best of our knowledge, this was the first study using whole-genome sequencing for Rhizobium sp. WYJ-E13 associated with C. wenyujin. WYJ-E13 strain has a high potential to be used as Curcuma biofertilizer for sustainable agriculture.

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Immobilization of an endophytic Bacillus sp. on Phragmites karka stem for lipase production in the presence of Cassia fistula seeds

Ashraf,

Sohail,

Abideen

et al. 2024

Biofuels Bioprod Bioref

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Endophytic bacteria have not been reported widely for their lipolytic abilities, so they are not used for large‐scale lipase production. The purpose of this study was to explore an endophytic bacterium for the production of lipase implementing cost‐effective techniques, including the use of Cassia fistula seeds, as a substrate in the production medium and stem pieces of Phragmites karka as the immobilization matrix. The endophytic strain Bacillus sp. E4 was originally isolated from the halophytic plant Arthrocnemum macrostachyum. Bacillus sp. E4 produced 6.05 IU mL−1 lipase in the presence of powdered seeds of Cassia fistula (golden shower tree). Initial trial experiments using a one‐factor‐at‐a‐time approach led to an improvement in lipase titers to 10.05 IU mL−1. Consequently, investigations using the Plackett–Burman design suggested the influence of three significant factors – incubation period, inoculum size, and substrate concentration – on lipase production. They were optimized using the Box–Behnken design (BBD). In the response optimization experiment, strain E4 yielded 52.35 IU mL−1 lipase, which was in accordance with the predicted yield and indicated an overall 8.65 fold improvement in lipase production. To investigate the use of free cells, strain E4 was immobilized on the stem pieces of a halophytic plant, Phragmites karka, which was used for the first time as an immobilization matrix. The immobilized cells retained lipase production ability for up to six cycles with the highest yield of 110 IU mL−1, which corresponded to an improvement of more than eighteenfold. Scanning electron micrographs confirmed the colonization of E4 cells in the matrix and demonstrated the utilization of C. fistula seeds. Fourier transform infrared spectroscopy affirmed the utilization of components including fatty acids by the immobilized E4 cells. The study suggests that endophytic bacterial strains could be applied for the production of lipase with the utilization of nontraditional oil sources.

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Bacillus altitudinis HNH7 and Bacillus velezensis HNH9 promote plant growth through upregulation of growth-promoting genes in upland cotton

Cited by 23 publications

References 58 publications

Bacillus thuringiensisCHGP12 uses a multifaceted approach for the suppression of Fusarium oxysporum f. sp. ciceris and to enhance the biomass of chickpea plants

Bacillus thuringiensisCHGP12 uses a multifaceted approach for the suppression of Fusarium oxysporum f. sp. ciceris and to enhance the biomass of chickpea plants

Deciphering the potential of a plant growth promoting endophyte Rhizobium sp. WYJ-E13, and functional annotation of the genes involved in the metabolic pathway

Immobilization of an endophytic Bacillus sp. on Phragmites karka stem for lipase production in the presence of Cassia fistula seeds

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