Bacillus catenulatus sp. nov., an alkalitolerant bacterium isolated from a soda lake

Abstract

Two novel (18C and 6C) Gram-stain-positive, rod shaped, motile and endospore-forming bacterial strains were isolated from Lonar soda lake, India. Based on 16S rRNA gene sequence analysis, strains 18C and 6C were identified as belonging to the class Firmibacteria, and were most closely related to Bacillus cohnii KCTC 3572 (99.3 and 99.9%, respectively), Bacillus zhanjiangensis KCTC 13713 (97.4 and 98.0%, respectively), Bacillus halmapalus LMG 17950 (97.0 and 97.6%, respectively) and other members in the genus Bacillus (<97.0%). However, the DNA-DNA relatedness between 18C and 6C and B. cohnii KCTC 3572 (49.6 ± 0.9 and 51.6 ± 0.7, respectively), B. zhanjiangensis KCTC 13713 (42.9 ± 0.8 and 47.1 ± 0.3, respectively) and B. halmapalus LMG 17950 (39.9 ± 0.8 and 40.8 ± 0.3, respectively) indicated that the novel strains were distantly related to these strains. Further, the high 16S rRNA gene sequence similarity (100%) and DNA-DNA relatedness (90 ± 5%) suggested that strains 18C and 6C were members of a genomospecies. The strains grew optimally at a pH of 7.5 with 2-3% (w/v) NaCl and temperature of 37 °C. Strains 18C and 6C were catalase and oxidase negative. The cell wall of strain 18C contained meso-diaminopimelic acid as the diagnostic diamino acid, which was in contrast with its nearest neighbour B. cohnii KCTC 3572, which contained ornithine and aspartic acid. Polar lipids include diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), phosphatidylethanolamine (PE), an unknown phospholipid (PL) and three unknown lipids (L1-3). The predominant isoprenoid quinone was MK-7. iso-C (32.5%) was the predominant fatty acid and significant proportions of anteiso-C (19.5%), C (11.5%), iso-C (9.5%) and anteiso-C (6.3%) were also detected. The DNA G + C content of strains 18C and 6C were 39.3 and 39.2 mol%, respectively. The results of molecular, biochemical and chemotaxonomic tests showed a clear differentiation of strains 18C and 6C from all other members of the genus Bacillus, for which the name Bacillus catenulatus sp. nov. is proposed. The type strain is 18C (=KCTC 33781 = CGMCC 1.15475).

Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species

“…Type strain: KCTC 3912=BH169=DSM 16461==CIP 109218. The description is modified from that given by Jiang et al [65] and Sultanpuram et al [93]. Facultative anaerobes and Gram-stain-positive rods.…”

“…Basonym: Bacillus catenulatus Sultanpuram et al 2018 The description of this species is the same as provided by Sultanpuram [93] for Bacillus catenulatus.…”

Identification and Characterization of a 2,2-Dichloropropionic Acid (2,2-DCP) Degrading Alkalotorelant Bacterium Strain BHS1 Isolated from Blue Lake, Turkey

“…This supports our study, during which we were also able to isolate Bacillus genus from the same environment. The previously reported halotolerant halogen-degrading Bacillus sp., Bacillus zhanjiangensis, Bacillus cohnii, and Bacillus halmapalus were isolated from Indian Lonar Soda Lake which showed an optimum growth at pH 7.5 and 37°C [50].…”

Sutcliffiella deserti sp. nov., isolated from desert soil

“…The DNA G+C content is within the range of 38.6-41.3 mol%. Isolates from soil [2][3][4] and oyster [5] have been reported for some species.…”