Bacillus Cereus Food Poisoning: A Provisional Serotyping Scheme

Taylor, A. J.; Gilbert, Richard J.

doi:10.1099/00222615-8-4-543

Cited by 82 publications

(50 citation statements)

References 7 publications

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“…In contrast, many cereulide-nonproducing strains could not be assigned to any of the 18 established serotypes described by Taylor and Gilbert (19), except for a few strains belonging to serotype H11. When tested for enterotoxin production, all cereulide-nonproducing strains were HBL positive, whereas all cereulide-producing strains were HBL negative (Table 1 and 2).…”

Section: Resultsmentioning

confidence: 78%

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A New Phylogenetic Cluster of Cereulide-Producing Bacillus cereus Strains

et al. 2007

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Phenotypic and molecular studies have established that cereulide-producing strains of Bacillus cereus are a distinct and probably recently emerged clone within the Bacillus population. We analyzed a set of B. cereus strains, both cereulide producers and nonproducers, by multilocus sequence typing. Consistent with earlier reports, nonproducers demonstrated high heterogeneity. Most cereulide-producing strains and all flagellar antigen type H1 strains were allocated to the known sequence type of exclusively emetic B. cereus strains. Several cereulide-producing strains, however, were recovered at a new phylogenetic location, all of which were serotype H3 or H12. We hypothesize that the group of cereulide producers is diversifying progressively, probably by lateral transfer of the corresponding gene complex.

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Section: Resultsmentioning

confidence: 78%

“…These strains were isolated between 1974 and 2005, mainly in Japan (Tables 1 and 2). Flagellar antigen serotyping was performed by the H antigen agglutination method described by Taylor and Gilbert (19).…”

Section: Methodsmentioning

confidence: 99%

A New Phylogenetic Cluster of Cereulide-Producing Bacillus cereus Strains

et al. 2007

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“…cereus isolates were referred for con®rmation and typing to the PHLS Food Safety Microbiology Laboratory, Central Public Health Laboratory, Colindale, London. Both isolates were con®rmed as B. cereus as described previously [13]; were non-serotypable [14,15], and showed an indistinguishable ampli®ed fragment length polymorphism (AFLP) pro®le (Fig. 1) [16].…”

Section: Molecular and Serological Typingmentioning

confidence: 99%

Bacillus cereus cellulitis from contaminated heroin

Dancer¹,

McNair²,

Finn

et al. 2002

Journal of Medical Microbiology

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Concern exists over recent unexplained deaths among intravenous drug users. This report describes a patient with crepitant cellulitis who was admitted complaining of severe pain in the right forearm. Ultrasonography demonstrated gas in the tissues and he was referred for early surgical debridement of the arm. He was treated with intravenous benzyl penicillin, gentamicin and metronidazole and made a full recovery. Aspirate samples grew Bacillus cereus, morphologically similar to the isolate obtained from a sample of the patient's own heroin. Antibiogram and API 50CHB pro®les were also similar. Further typing included`H'¯agellar serotyping, which found both blood and heroin strains to be non-typable, and ampli®ed fragment polymorphism analysis, which showed that the strains were indistinguishable. Genotyping of two selected genes from B. cereus con®rmed almost certain identity between the two strains. This case illustrates the potential virulence of B. cereus when inoculated into tissues, and to our knowledge, is the ®rst report to demonstrate a conclusive microbiological link between contaminated heroin and serious sepsis in a drug user due to B. cereus.

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“…One is a diarrheal-type syndrome which results in diarrhea and abdominal pain 8-16 hr after the ingestion of contaminated foods. The other is a vomiting-type syndrome which is characterized by nausea and vomiting within 1-5 hr of ingestion (11,13). To facilitate the identification of outbreaks of food poisoning by B. cereus, detection of the emetic toxin in the suspected food source is desirable.…”

mentioning

confidence: 99%

Growth Conditions of and Emetic Toxin Production by Bacillus cereus in a Defined Medium with Amino Acids

Agata¹,

Ohta

Mori³

et al. 1999

Microbiology and Immunology

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The growth and emetic toxin (cereulide) production of Bacillus cereus strains in defined culture media were studied. We found that a fully synthetic medium (CADM) allowed the production of emetic toxin and the addition of glucose enhanced it. By subtracting each amino acid from CADM, we found that only three amino acids, valine, leucine and threonine, were essential for growth and toxin production by B. cereus. The addition of high levels (50 mm) of leucine, isoleucine and glutamic acid decreased the toxin production. Other amino acids had no effect at this concentration.

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Bacillus Cereus Food Poisoning: A Provisional Serotyping Scheme

Cited by 82 publications

References 7 publications

A New Phylogenetic Cluster of Cereulide-Producing Bacillus cereus Strains

A New Phylogenetic Cluster of Cereulide-Producing Bacillus cereus Strains

Bacillus cereus cellulitis from contaminated heroin

Growth Conditions of and Emetic Toxin Production by Bacillus cereus in a Defined Medium with Amino Acids

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