Bacillus coagulans MZY531 alleviates intestinal mucosal injury in immunosuppressive mice via modulating intestinal barrier, inflammatory response, and gut microbiota

Abstract

Bacillus coagulans has a potential role in improving intestinal injury. However, the specific mechanism is still unclear. In this study, the protective effect of B. coagulans MZY531 on intestinal mucosa injury in cyclophosphamide (CYP)-induced immunosuppressed mice were investigated. The results indicated that the immune organ (thymus and spleen) indices of B. coagulans MZY531 treatment groups were significantly increased compared to the CYP group. B. coagulans MZY531 administration promotes the expression of immune proteins (IgA, IgE, IgG, and IgM). B. coagulans MZY531 could upregulate the ileum levels of IFN-γ, IL-2, IL-4, and IL-10 in immunosuppressed mice. Moreover, B. coagulans MZY531 restores the villus height and crypt depth of the jejunum and alleviates injury of intestinal endothelial cells caused by CYP. Furthermore, the western blotting results showed that B. coagulans MZY531 ameliorated CYP-induced intestinal mucosal injury and inflammatory via up-regulates the ZO-1 pathway and down-regulates the expression of the TLR4/MyD88/NF-κB pathway. After treatment with B. coagulans MZY531, the relative abundance of Firmicutes phylum was dramatically increased, as well as the genera of Prevotella and Bifidobacterium, and reducing harmful bacteria. These findings suggested that B. coagulans MZY531 has a potential immunomodulatory activity on chemotherapy-induced immunosuppression.

Effect of bovine colostrum liposomes on the bioavailability of immunoglobulin G and their immunoregulatory function in immunosuppressed BALB/c mice

“…CTX is widely used in treating various cancer and allergic diseases; however, it can damage the immune system, induce disruption of the gut microbiota, and increase the risk of immunodeficiency and intestinal injury diseases, which ultimately can cause adverse effects on the immune system; therefore, CTX is also used in the establishment of immunosuppressed models. 29–31 As important immune organs, the spleen and thymus play crucial roles in regulating immune function. Consistent with the investigations of other researchers, 32–34 our current research indicated that CTX could decrease the body weight, spleen weight, and thymus weight in BALB/c mice, which indicated that CTX could cause damage to the immune system in the mice; however, the supplementation of BC encapsulated by liposomes improved these adverse alterations and exhibited a better effect than BC alone (Fig.…”

Weizmannia coagulans BCF-01: a novel gastrogenic probiotic for Helicobacter pylori infection control

“…These findings align with previous reports of W. coagulans strains colonizing the intestinal mucosa, whereby they enhance mucosal barrier function, modulate the mucosal immune system, reduce inflammatory factor production, alleviate intestinal inflammation and inflammation-related diseases, promote secretory IgA production, and facilitate antigen-specific immune responses. 41 …”

Progress of research and application of Heyndrickxia coagulans (Bacillus coagulans) as probiotic bacteria

“…This process not only optimizes the structure of the microbial community but also enhances the host’s absorption and utilization of inorganic trace elements through the formation of organic mineral salts. H. coagulans also secretes specialized adhesion proteins, including mucins and fibrinogen-binding proteins, which facilitate colonization in the gut and reduce pathogenic adhesion ( Zhao et al., 2023 ).…”