Bacillus cucumis sp. nov. isolated from the rhizosphere of cucumber (Cucumis sativus)

Kämpfer, Peter; Busse, Hans‐Jürgen; Glaeser, Stefanie P.; Kloepper, Joseph W.; Hu, Chia-Hui; McInroy, John A.

doi:10.1099/ijsem.0.000831

Cited by 16 publications

(8 citation statements)

References 30 publications

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“…At the time of writing, 16 species were allocated to this genus. Most of the type strains of Neobacillus species were isolated from soil [2][3][4][5][6], including root-associated [7] and specifically rhizosphere soil [8], as well as root endophytes [9,10]. Single type strains were also isolated from compost [11], deep sea sediment [12], and human faeces [13].…”

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confidence: 99%

Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., isolated from maize root rhizosphere

Kämpfer

Glaeser

Busse³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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A facultative anaerobic, Gram-stain-positive, endospore-forming bacterium, isolated from the rhizosphere of maize roots (Zea mays), was taxonomically studied. Based on 16S rRNA gene sequence similarity comparisons, strain JJ-79T clustered only loosely with Neobacillus species and showed the highest similarity to Neobacillus soli (97.9%). The 16S rRNA gene sequence similarities to the sequences of the type strains of other Neobacillus species were 97.5 % and below. Chemotaxonomic features supported the grouping of the strain to the Neobacillus group, e.g. the major fatty acids were C15 : 0 anteiso, C15 : 0 iso and C16 : 0, the polar lipid profile contained the major components diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified glycolipid, the major quinone was menaquinone MK-7, and major compound in the polyamine pattern was spermidine. However, the JJ-79T genome assembly did not share most of the 11 conserved signature indels that are indicative of the genus Neobacillus . The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between the JJ-79T genome assembly and those of the closest relative Bacillaceae type strains were <71, <71 and <25 %, respectively. Physiological and biochemical test results were also different from those of the most closely related Bacillaceae species. As a consequence, JJ-79T represents a novel genus for which we propose the name Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., with JJ-79T (=CIP 111885T=CCM 9045T) as the type strain.

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confidence: 99%

Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., isolated from maize root rhizosphere

Kämpfer

Glaeser

Busse³

et al. 2022

International Journal of Systematic and Evolutionary Microbiology

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“…de/ genus/ neobacillus). Species of this genus have been isolated from diverse environmental niches, including soil [6][7][8], windrow compost [9], plant roots [10][11][12] and human faecal matter [13]. Members of the genus are Gram-stain-positive or -variable and facultative anaerobes or aerobes [6][7][8][9][10]13].…”

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confidence: 99%

Neobacillus endophyticus sp. nov., an endophytic bacterium isolated from Selaginella involvens roots

Jiang

Lee

Jeong

et al. 2019

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-positive, facultatively anaerobic, rod-shaped, endospore-forming, oxidase-positive, and catalase-negative strain designated as BRMEA1T was isolated from the surface-sterilized Selaginella involvens roots. Growth of strain BRMEA1T was found to occur at pH 6.0–8.0 (optimum, pH 7.0), 15–50 °C (optimum, 25–30 °C) and in the absence of NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain BRMEA1T formed a lineage within the genus Neobacillus (family Bacillaceae ) and showed the highest sequence similarity to Neobacillus drentensis DSM 15600T (98.3 %) and Neobacillus fumarioli KCTC 13885T (98.2 %), and less than 98.2 % 16S rRNA gene sequence similarity to the other members of the genus Neobacillus . Whole-genome analysis of strain BRMEA1T comprised a circular chromosome (5 632 809 bp in size) with 38.5 mol% G+C content. Digital DNA–DNA hybridization analyses revealed that strain BRMEA1T showed 20.5 and 22.0% genomic DNA relatedness with the closest species, N. drentensis DSM 15600T and N. fumarioli KCTC 13885T, respectively. The whole-genome sequence of strain BRMEA1T showed the presence of 11 specific conserved signature indels for the genus Neobacillus . The major cellular fatty acids (>10 %) of strain BRMEA1T were found to be iso-C15 : 0 and anteiso-C15 : 0, while the major polar lipids were found to be diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Polyphasic analysis results revealed that BRMEA1T represents a novel species of the genus Neobacillus , with the proposed name Neobacillus endophyticus sp. nov. The type strain is BRMEA1T (=KCTC 43208T=CCTCC AB 2020071T).

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“…At the time of writing, in the genus Neobacillus , there were 16 species with validly published names included in the List of Prokaryotic names with Standing in Nomenclature (https://lpsn.dsmz.de/genus/neobacillus). The members of this genus can be found in a wide variety of environments, including soil [2–6], plant root [7–10], windrow compost [11], deep-sea [12] and human faeces [13]. Most members of this genus are Gram-stain-positive or variable, aerobic or facultatively anaerobic, motile or non-motile, endospore-forming, rod-shaped, and the maximum temperature at which they can grow is around 45 °C and above.…”

Section: Full-textmentioning

confidence: 99%

Neobacillus kokaensis sp. nov., isolated from soil

Yuki

Matsubara²,

Yamaguchi³

2022

International Journal of Systematic and Evolutionary Microbiology

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A Gram-stain-variable, motile, aerobic, spore-forming, rod-shaped bacterium, designated as strain LOB 377T, was isolated from soil sampled in Koka County (now Konan City), in Shiga Prefecture, Japan. To determine its taxonomic position, the bacterium was evaluated by a polyphasic approach based on genomic, phenotypic and chemotaxonomic tests. From phylogenetic analysis based on 16S rRNA gene sequences, strain LOB 377T (LC570960) was revealed to have the highest similarity to the type strain of Neobacillus mesonae FJAT-13985T (9.1 %). The average nucleotide identity and digital DNA–DNA hybridization values between both strains based on whole genome sequences were 84.1 and 42.8 %, respectively, which were below the recommended thresholds. The strain grew at 15–45 °C (optimum, 25–37 °C), at pH 5.5–9.5 (optimum, pH 6.5–8.5) and with 1.0–2.0 % (w/v) NaCl. Cell-wall peptidoglycan of the strain contained meso-diaminopimelic acid. The major menaquinone was menaquinone-7. Predominant fatty acids were iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0 and C16 : 1 ω11c. The polar lipids were phosphatidylglycerol, unidentified phospholipid and unidentified aminophospholipid. The DNA G+C content was 40.5 mol%. According to the genomic, phenotypic and chemotaxonomic results, strain LOB 377T represents a novel species in the genus Neobacillus , for which the name Neobacillus kokaensis sp. nov. is proposed. The type strain is LOB 377T (=ATCC 31382T=NBRC 114637T=DSM 113418T).

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Bacillus cucumis sp. nov. isolated from the rhizosphere of cucumber (Cucumis sativus)

Cited by 16 publications

References 30 publications

Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., isolated from maize root rhizosphere

Pseudoneobacillus rhizosphaerae gen. nov., sp. nov., isolated from maize root rhizosphere

Neobacillus endophyticus sp. nov., an endophytic bacterium isolated from Selaginella involvens roots

Neobacillus kokaensis sp. nov., isolated from soil

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