Bacillus filamentosus sp. nov., isolated from sediment sample

Abstract

A novel Gram-stain positive, endospore-forming bacterium, designated SGD-14(T), was isolated from a marine sediment sample in Goa Province, India. Cells of the isolate were found to be strictly aerobic. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SGD-14(T) showed a similarity of 99.5 % with Bacillus endophyticus and similarities to other Bacillus type strains were below 96 %. The whole-cell sugar pattern was found to consist of ribose, xylose and glucose. The predominant menaquinone was identified as MK-7 and the major fatty acids as anteiso-C15:0, iso-C15:0, iso-C16:0, anteiso-C17:0, C16:0 and iso-C14:0. The strain was found to grow optimally at 30 °C and pH 7.0-7.5. DNA G + C content was determined to be 39.6 mol%. The phospholipid pattern was found to consist of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. DNA-DNA hybridization studies between strain SGD-14(T) and B. endophyticus CIP106778(T) showed that strain SGD-14(T) exhibited <70 % similarity with B. endophyticus CIP106778(T). Differences in phenotypic and chemotaxonomic characteristics between the novel strain and B. endophyticus CIP106778(T) further confirmed that this isolate represents novel species. Phylogenetic analysis showed that strain SGD-14(T) fits in the same clade with B. endophyticus with 100 % bootstrap values. Strain SGD-14(T) is therefore considered to represent a novel species of the genus Bacillus, for which the name Bacillus filamentosus sp. nov. is proposed. The type strain of Bacillus filamentosus is SGD-14(T) = (=NCIM 5491(T) = DSM 27955(T)).

ACC Deaminase-Containing Rhizobacteria from Rhizosphere of Zygophyllum coccineum Alleviate Salt Stress Impact on Wheat (Triticum aestivum L.)

“…ZCA4 was an endosporeforming bacterium. These observations are in agreement with those of Bacillus filamentosus SGD-14 T (Sonalkar et al, 2015), Janibacter indicus 0704P10-1 T (Zhang et al,2014), and Brevibacterium casei NCDO 2048 (CMD1) (Collins et al,1983).…”

“…These testers can be used to easily differentiate between the strains. The observation that ZCA4 consumed an array of different carbon and nitrogen sources is in general agreement with that reported for B. filamentosus SGD-14 T (Sonalkar et al,2015), J. indicus 0704P10-1 T (Zhang et al,2014), and B. casei NCDO 2048 (CMD1) (Collins et al,1983). However, some differences do exist between our strains and the relevant type strains.…”

Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species

“…Reva et al 2002 The description of this species is the same as provided by Reva et al [134] for Bacillus endophyticus. The description of this species is the same as provided by Sonalkar et al [135] for Bacillus filamentosus.…”

Bacillus crescens sp. nov., isolated from soil

“…Members of the genus Bacillus are a diverse group of bacteria in the phylum Firmicutes . At the time of writing, there are 18 species reported from India, of which 17 have validly published names, including Bacillus filamentosus (Sonalkar et al , 2015), Bacillus lonarensis (Reddy et al , 2015), Bacillus enclensis (Dastager et al , 2014) and Bacillus rhizosphaerae (Madhaiyan et al , 2011), while one species, ‘ Bacillus mesophilum ’ (Manickam et al , 2014), does not yet have a validly published name. Members of the genus Bacillus are characterized by Gram-stain-positive, oval to rod-shaped cells, aerobic or facultative anaerobic growth and endospore formation.…”