Bacillus manliponensis sp. nov., a new member of the Bacillus cereus group isolated from foreshore tidal flat sediment

Abstract

A Gram-positive, endospore-forming, new Bacillus species, strain BL4-6(T), was isolated from tidal flat sediment of the Yellow Sea. Strain BL4-6(T) is a straight rod, with motility by peritrichate flagella. The cell wall contains meso-diaminopimelic acid, and the major respiratory quinone is menaquinone-7. The major fatty acids are iso-C(15:0) and summed feature 3 (containing C(16:1) ω7c/iso-C(15:0) 2OH, and/or iso-C(15:0) 2OH/C(16:1) ω7c). Cells are catalase-positive and oxidase-negative. The G+C content of the genomic DNA is 38.0 mol%. Based on a comparative 16S rRNA gene sequence analysis, the isolate belongs to the genus Bacillus, forms a clade with the Bacillus cereus group, and is closely related to Bacillus mycoides (98.5%), Bacillus cereus (98.5%), Bacillus anthracis (98.4%), Bacillus thuringiensis (98.4%), Bacillus weihenstephanensis (98.1%), and Bacillus pseudomycoides (97.5%). The isolate showed less than 85% similarity of the gyrA gene sequence and below 95% similarity of the rpoB gene sequence to the members of this group. DNA-DNA relatedness between strain BL4-6(T) and B. cereus group was found to be in a range of 22.8-42.3%, and thus BL4-6(T) represents a unique species. On the basis of these studies, strain BL4-6(T) (=KCTC 13319(T) =JCM 15802(T)) is proposed to represent the type strain of a novel species, Bacillus manliponensis sp. nov.

The Identification of Intrinsic Chloramphenicol and Tetracycline Resistance Genes in Members of the Bacillus cereus Group (sensu lato)

“…cereus ( sensu stricto ), B. anthracis, B. thuringiensis, B. mycoides, B. pseudomycoides, B. weihenstephanensis, B. cytotoxicus , and B. toyonensis (Skerman et al, 1980; Lechner et al, 1998; Guinebretière et al, 2013; Jiménez et al, 2013). Three additional new species, B. gaemokensis, B. manliponensis , and B. bingmayongensis , have been recently reported to belong to B. cereus group, but their names are not currently validly published (Jung et al, 2010, 2011; Liu et al, 2014, 2015). …”

Bacillus wiedmannii sp. nov., a psychrotolerant and cytotoxic Bacillus cereus group species isolated from dairy foods and dairy environments

“…gaemokensis' (Jung et al, 2010), 'B. manliponensis' (Jung et al, 2011) and 'B. bingmayongensis' (Liu et al, 2014)], but have not yet been validly published.…”

Characterization of acidophilic bacteria related to Acidiphilium cryptum from a coal-mining-impacted river of South Brazil

“…Although 16S gene sequences are the most routinely used method of identification of bacteria, it is not always reliably to differentiate at the species level (Logan & Halket, 2011). Some bacteria, like the members of the Bacillus cereus group, have almost identical 16S gene sequences, being differentiated by its physiological and virulence characteristics (Jung et al, 2011). On the Figure 3 -Growth of the three bacterial strains isolated from acid samples on increasing concentrations of the metals nickel, copper, and zinc, measure as optical density at 600nm.…”