Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands

Heyrman, Jeroen; Vanparys, Bram; Logan, Niall A.; Balcaen, Annelies; Rodríguez‐Díaz, Marina; Felske, Andreas; Vos, Paul De

doi:10.1099/ijs.0.02723-0

Cited by 131 publications

(74 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The cellular fatty acids of strain 17-5 T were as follows: anteiso-C 15 : 0 (30.6 %), C 16 : 0 (16.4 %), anteiso-C 17 : 0 (16.2 %), iso-C 16 : 0 (15.1 %), iso-C 15 : 0 (6.8 %), iso-C 14 : 0 (4.7 %), iso-C 17 : 0 (4.7 %), C 15 : 0 (2.9 %) and C 17 : 0 (2.4 %). These chemotaxonomic features of strain 17-5 T were typical of those found in members of the genus Bacillus (Priest et al, 1988;Heyrman et al, 2004Heyrman et al, , 2005Albert et al, 2005;Wieser et al, 2005;Lim et al, 2006a, b). The genomic DNA G+C content of strain 17-5 T was 48.1 mol%.…”

mentioning

confidence: 68%

Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China

Xue

Ventosa

Wang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

A Gram-positive, halophilic bacterium was isolated from a sediment sample from Ai-Ding salt lake in China. The isolate, designated strain 17-5 T , grew at salinities of 8-33 % (w/v) NaCl (optimally at 12 %, w/v). The genomic DNA G+C content of strain 17-5 T was 48.1 mol%. The predominant isoprenoid quinone was MK-7(H 2 ) and the cell-wall peptidoglycan contained mesodiaminopimelic acid. The major polar lipids were diphosphatidylglycerol and an unidentified glycolipid. The major cellular fatty acids were anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 16 : 0 and C 16 : 0 . Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 17-5 T was a member of the genus Bacillus, being most closely related to Bacillus qingdaonensis JCM 14087 T (96.0 % sequence similarity) and Bacillus salarius DSM 16461 T (95.6 %). The levels of 16S rRNA gene sequence similarity with respect to other Bacillus species were less than 91.7 %. Comparative analysis of the 16S rRNA gene sequence data, chemotaxonomy and phenotypic features of the novel isolate and related species of Bacillus indicated that strain 17-5 T represents a novel species within the genus Bacillus, for which the name Bacillus aidingensis sp. nov. is proposed. The type strain is 17-5 T (5CGMCC 1.3227 T 5DSM 18341 T ).Moderately halophilic bacteria that grow optimally in media containing 3-15 % (w/v) NaCl are widely distributed throughout various types of saline environments, such as salt lakes, salterns and salty foods (Ventosa et al., 1998). Lake Ai-Ding (89 u 109 320-83 u 549 320 E 42 u 329 100-42 u 499 130 N) is a typical chloride-sulphate saline lake with a neutral pH and a salt concentration of 20-26 % (w/v). Previous studies on the microbial diversity of Ai-Ding salt lake have demonstrated the presence of a variety of halophilic micro-organisms (Cui et al., 2006a, b; Ren & Zhou, 2005a, b). In this paper, we describe a novel moderately halophilic bacterium, designated strain 17-5 T , isolated from Lake Ai-Ding. Phenotypic and chemotaxonomic characteristics, as well as data from a phylogenetic analysis based on 16S rRNA gene sequence comparisons, showed that strain 17-5 T represents a novel species within the genus Bacillus.The sample collection/treatment and the enrichment and isolation of strain 17-5 T were performed as described by Ren & Zhou (2005a). Strain 17-5 T was routinely grown on HM medium (Ventosa et al., 1982) containing 12 % (w/v) NaCl instead of 17.8 % NaCl (modified HM medium). Cellular morphology was examined using light microscopy and transmission electron microscopy. Gram staining was performed as described by Gerhardt et al. (1981), in parallel with the KOH test (Gregersen, 1978). Motility was determined in wet mounts by using phase-contrast microscopy; flagella were demonstrated using negative staining (Kodaka et al., 1982) and transmission electron microscopy. The NaCl, temperature and pH ranges for growth, the utilization of carbon and energy sources (added at 0.5 %, w/v) and the hydrolysis of starch, casein, gelatin and Tweens 20,...

show abstract

mentioning

confidence: 68%

Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China

Xue

Ventosa

Wang

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

View full text Add to dashboard Cite

show abstract

“…and (iv) a Pseudomonas sp., which all have been reported to be endophytic bacteria in pea 19 , a member of the Fabaceae, like yellow lupine; and (v) a Bacillus novalis sp. nov., which was recently isolated from the Drentse A grasslands 20 . None of these bacteria was able to grow on toluene as a carbon source.…”

Section: Inoculation Of Yellow Lupine With B Cepaciamentioning

confidence: 99%

Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants

et al. 2004

View full text Add to dashboard Cite

Phytoremediation of highly water soluble and volatile organic xenobiotics is often inefficient because plants do not completely degrade these compounds through their rhizospheres. This results in phytotoxicity and/or volatilization of chemicals through the leaves, which can cause additional environmental problems. We demonstrate that endophytic bacteria equipped with the appropriate degradation pathway improve the in planta degradation of toluene. We introduced the pTOM toluene-degradation plasmid of Burkholderia cepacia G4 into B. cepacia L.S.2.4, a natural endophyte of yellow lupine. After surface-sterilized lupine seeds were successfully inoculated with the recombinant strain, the engineered endophytic bacteria strongly degraded toluene, resulting in a marked decrease in its phytotoxicity, and a 50-70% reduction of its evapotranspiration through the leaves. This strategy promises to improve the efficiency of phytoremediating volatile organic contaminants.

show abstract

“…Phenotypic tests were performed as described by Logan & Berkeley (1984) and Heyrman et al (2004); other characters were determined and the data analysed numerically using the general similarity coefficient of Gower (S G ) as described by Logan et al (2000). For observations on sporangia, cells were grown on tryptic soy agar (TSA) supplemented with 5 mg MnSO 4 l 21 at 30 uC.…”

Section: Methodsmentioning

confidence: 99%

Bacillus farraginis sp. nov., Bacillus fortis sp. nov. and Bacillus fordii sp. nov., isolated at dairy farms

Scheldeman

Rodríguez‐Díaz

Goris

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

Self Cite

View full text Add to dashboard Cite

Forty-eight bacterial strains were isolated at dairy farms from raw milk, the milking apparatus, green fodder or feed concentrate after a heat treatment of 30 min at 100 °C. In this way, spore-forming bacteria with a very high intrinsic heat resistance were selected for. The aerobic spore-forming isolates were subjected to a polyphasic taxonomical study, including repetitive element sequence-based PCR typing, whole-cell protein profiling, 16S rDNA sequence analysis, DNA–DNA hybridizations, DNA base composition, fatty acid analysis, and morphological and biochemical characteristics. A comparison of the REP- and (GTG)5-PCR and whole-cell protein SDS-PAGE profiles resulted in three clusters of similar strains. Analysis of the 16S rDNA sequences and DNA–DNA relatedness data showed that these clusters represented three novel species. The highest 16S rDNA similarity to a recognized species found for the three groups was around 94 % with Bacillus lentus and Bacillus sporothermodurans. Further phenotypic characterization supported the proposal of three novel species in the genus Bacillus, Bacillus farraginis, Bacillus fortis and Bacillus fordii. The respective type strains are R-6540T (=LMG 22081T=DSM 16013T), R-6514T (=LMG 22079T=DSM 16012T) and R-7190T (=LMG 22080T=DSM 16014T); their G+C DNA base contents are 43·7, 44·3 and 41·9 mol%, respectively. Although in variable amounts, a predominance of the branched fatty acids iso-C15 : 0 and anteiso-C15 : 0 was observed in all three novel species.

show abstract

Bacillus novalis sp. nov., Bacillus vireti sp. nov., Bacillus soli sp. nov., Bacillus bataviensis sp. nov. and Bacillus drentensis sp. nov., from the Drentse A grasslands

Cited by 131 publications

References 24 publications

Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China

Bacillus aidingensis sp. nov., a moderately halophilic bacterium isolated from Ai-Ding salt lake in China

Engineered endophytic bacteria improve phytoremediation of water-soluble, volatile, organic pollutants

Bacillus farraginis sp. nov., Bacillus fortis sp. nov. and Bacillus fordii sp. nov., isolated at dairy farms

Contact Info

Product

Resources

About