Bacillus pasinlerensis sp. nov., a thermophilic bacterium isolated from a hot spring in Turkey

To clarify the evolutionary relationships and classification of Bacillus species, comprehensive phylogenomic and comparative analyses were performed on >300 Bacillus/Bacillaceae genomes. Multiple genomic-scale phylogenetic trees were initially reconstructed to identify different monophyletic clades of Bacillus species. In parallel, detailed analyses were performed on protein sequences of genomes to identify conserved signature indels (CSIs) that are specific for each of the identified clades. We show that in different reconstructed trees, most of the Bacillus species, in addition to the Subtilis and Cereus clades, consistently formed 17 novel distinct clades. Additionally, some Bacillus species reliably grouped with the genera Alkalicoccus, Caldalkalibacillus, Caldibacillus, Salibacterium and Salisediminibacterium . The distinctness of identified Bacillus species clades is independently strongly supported by 128 identified CSIs which are unique characteristics of these clades, providing reliable means for their demarcation. Based on the strong phylogenetic and molecular evidence, we are proposing that these 17 Bacillus species clades should be recognized as novel genera, with the names Alteribacter gen. nov., Ectobacillus gen. nov., Evansella gen. nov., Ferdinandcohnia gen. nov., Gottfriedia gen. nov., Heyndrickxia gen. nov., Lederbergia gen. nov., Litchfieldia gen. nov., Margalitia gen. nov., Niallia gen. nov., Priestia gen. nov., Robertmurraya gen. nov., Rossellomorea gen. nov., Schinkia gen. nov., Siminovitchia gen. nov., Sutcliffiella gen. nov. and Weizmannia gen. nov. We also propose to transfer ‘ Bacillus kyonggiensi s’ to Robertmurraya kyonggiensis sp. nov. (type strain: NB22=JCM 17569T=DSM 26768). Additionally, we report 31 CSIs that are unique characteristics of either the members of the Subtilis clade (containing the type species B. subtilis ) or the Cereus clade (containing B. anthracis and B. cereus ). As most Bacillus species which are not part of these two clades can now be assigned to other genera, we are proposing an emended description of the genus Bacillus to restrict it to only the members of the Subtilis and Cereus clades.

show abstract

“…The description of this species is the same as provided by Baltaci et al [88] for Bacillus pasinlerensis.…”

Section: Discussionmentioning

confidence: 99%

Robust demarcation of 17 distinct Bacillus species clades, proposed as novel Bacillaceae genera, by phylogenomics and comparative genomic analyses: description of Robertmurraya kyonggiensis sp. nov. and proposal for an emended genus Bacillus limiting it only to the members of the Subtilis and Cereus clades of species

Gupta

Patel

Saini

et al. 2020

International Journal of Systematic and Evolutionary Microbiology

760

173

View full text Add to dashboard Cite

show abstract

“…Neighbor-joining phylogenetic tree on the basis of 16S rRNA gene sequence data of NGB15 was constructed using the MEGA4 program. Pallidibacillus pasinlerensis was used as an out-group (Baltaci et al 2020a ). Bootstrap values based on 1000 replications are listed as percentages at branching points.…”

Section: Resultsmentioning

confidence: 99%

Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Baltacı,

Görmez

et al. 2024

Environ Sci Pollut Res

View full text Add to dashboard Cite

Polyhydroxyalkanoates have attracted great interest as a suitable alternative to petrochemical based plastics due to their outstanding properties such as biodegradability and biocompatibility. However, the biggest problem in the production of microbial polyhydroxyalkanoates is low cost-effectiveness. In this study, polyhydroxyalkanoate production was carried out using waste substrates with local isolates. Culture conditions were optimized to increase the polyhydroxyalkanoate production potential. The produced polyhydroxyalkanoate was characterized by FTIR analyses, and its metabolic pathway was determined by real-time PCR. According to the results, the best polyhydroxyalkanoate producer bacteria was characterized as Pseudomonas neustonica NGB15. The optimal culture conditions were detected as 30 g/L banana peel powder, 25 °C temperature, pH 8, and 4-day incubation time. Under the optimized conditions, 3.34 g/L PHA production was achieved. As a result of FTIR analyses, major peaks were obtained at 1723, 1277, 1261, 1097, 1054, and 993 cm−1. These peaks represent that the type of produced polyhydroxyalkanoate was poly-β-hydroxybutyrate. According to gene expression profile of NGB15, it was determined that Pseudomonas neustonica NGB15 produces PHA using the de novo fatty acid synthesis metabolic pathway. In conclusion, poly-β-hydroxybutyrate production by Pseudomonas neustonica NGB15 using a low-cost fermentation medium has been shown to be biotechnologically promising.

show abstract

“…To determine phenotypic characterization, test strains were grown in MRS and M17 medium at different temperatures 15°C to 50°C (with 5°C intervals) for up to 72 h. The pH ranges were analyzed in MRS and M17 at pH 3.0-11.0 (1 pH unit intervals), and tolerance of NaCl was determined using MRS and M17 supplemented with 0-12 % NaCl (at intervals of 1.0 %) for 72 h. All experiments were tested in triplicate and growth was measured at OD600 nm. 32 Gram-staining of test strains was performed according to the method by Gerhardt et al 33 Catalase activity was performed by the production of bubbles of a drop of 3 % H 2 O 2 (v/v). Oxidase reagent (Sigma) was used for testing oxidase activity.…”

Section: Phenotypic Characterizationmentioning

confidence: 99%

Identification and Potential Biotechnological Characterization of Lactic Acid Bacteria Isolated from White Cheese Samples

Akbulut¹,

Baltaci²,

Adıgüzel³

et al. 2022

J. Pure Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

In this study, the isolation of lactic acid bacteria was carried out from one hundred white cheese samples collected from different regions of Turkey. Subsequently, phenotypic and genotypic characterization of the isolates was performed. Biochemical characteristics of the isolates were determined by API 50CHL. Furthermore, the biotechnological enzyme production potential of the isolates was screened. Genomic fingerprint profiles of the test isolates were detected by using rep-PCR (BOX-PCR), which has been used successfully in the differentiation of microorganisms at the species, subspecies, and even strain levels. The results showed that a total of forty-one bacteria were isolated and seventeen of which are found to be different species. The isolates generally grew at 4-6 pH values, 0-8% NaCl and 30-40°C. Later, isolates thought to be different species were identified by 16S rRNA gene sequence analyses. According to 16S rRNA sequence results, MA56 showed a 96.41% similarity match to Lentilactobacillus buchneri, it is thought to be a new species. In addition, MA19, MA25, MA43, and MA47 were determined to have multi-enzyme production potential. MA43 has a plantaricin gene and it showed a high antagonistic effect on Escherichia coli O157:H7 ATCC 43888 and Pseudomonas aeruginosa ATCC 9027. Inhibition zones were measured at 19 mm and 16 mm respectively.

show abstract

Bacillus pasinlerensis sp. nov., a thermophilic bacterium isolated from a hot spring in Turkey

Cited by 22 publications

References 49 publications

Green alternatives to petroleum-based plastics: production of bioplastic from Pseudomonas neustonica strain NGB15 using waste carbon source

Identification and Potential Biotechnological Characterization of Lactic Acid Bacteria Isolated from White Cheese Samples

Contact Info

Product

Resources

About