Bacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase

Frandi, Antonio; Zucca, Paolo; Marvasi, Massimiliano; Mastromei, Giorgio; Sanjust, Enrico; Perito, Brunella

doi:10.1007/s13213-010-0121-5

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…One of the enzymes isolated in the proteome obtained from cultures under standard conditions that participates in this process was the enoyl-CoA hydratase, which transforms a double bond into a single one and a hydroxyl radical by adding a water molecule ( Frandi et al, 2011 ).…”

Section: Resultsmentioning

confidence: 99%

Bacterial molecular machinery in the Martian cryosphere conditions

Muñoz-Hisado,

Ruiz-Blas,

Sobrado

et al. 2023

Front. Microbiol.

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The exploration of Mars is one of the main objectives of space missions since the red planet is considered to be, or was in the past, potentially habitable. Although the surface of Mars is now dry and arid, abundant research suggests that water covered Mars billions of years ago. Recently, the existence of liquid water in subglacial lakes has been postulated below the South pole of Mars. Until now, experiments have been carried out on the survival of microorganisms in Martian surface conditions, but it remains unknown how their adaptation mechanisms would be in the Martian cryosphere. In this work, two bacterial species (Bacillus subtilis and Curtobacterium flacumfaciens) were subjected to a simulated Martian environment during 24 h using a planetary chamber. Afterward, the molecular machinery of both species was studied to investigate how they had been modified. Proteomes, the entire set of proteins expressed by each bacterium under Earth (named standard) conditions and Martian conditions, were compared using proteomic techniques. To establish this evaluation, both the expression levels of each protein, and the variation in their distribution within the different functional categories were considered. The results showed that these bacterial species followed a different strategy. The Bacillus subtilis resistance approach consisted of improving its stress response, membrane bioenergetics, degradation of biomolecules; and to a lesser extent, increasing its mobility and the formation of biofilms or resistance endospores. On the contrary, enduring strategy of Curtobacterium flacumfaciens comprised of strengthening the cell envelope, trying to protect cells from the extracellular environment. These results are especially important due to their implications for planetary protection, missions to Mars and sample return since contamination by microorganisms would invalidate the results of these investigations.

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Section: Resultsmentioning

confidence: 99%

Bacterial molecular machinery in the Martian cryosphere conditions

Muñoz-Hisado,

Ruiz-Blas,

Sobrado

et al. 2023

Front. Microbiol.

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“…For metabolomics to be successfully applied to the discovery of new potentially useful organisms, however, it is necessary to better understand the biochemical processes involved in carbonatogenesis. Earlier genetic studies have, for example, used mutants impaired in CaCO 3 precipitation to indicate a link between biomineralization, fatty acid metabolism, altered phospholipid membrane composition and surface properties [8,31,[62][63][64]. Fatty acid metabolism has also been implicated by work that showed how CaCO 3 precipitation in Lysinibacillus could modify membrane rigidity by upregulating branched chain fatty acid synthesis [65,66].…”

Section: Figurementioning

confidence: 99%

Bioconservation of Historic Stone Buildings—An Updated Review

Ortega-Morales

Gaylarde

2021

Applied Sciences

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Cultural heritage buildings of stone construction require careful restorative actions to maintain them as close to the original condition as possible. This includes consolidation and cleaning of the structure. Traditional consolidants may have poor performance due to structural drawbacks such as low adhesion, poor penetration and flexibility. The requirement for organic consolidants to be dissolved in volatile organic compounds may pose environmental and human health risks. Traditional conservation treatments can be replaced by more environmentally acceptable, biologically-based, measures, including bioconsolidation using whole bacterial cells or cell biomolecules; the latter include plant or microbial biopolymers and bacterial cell walls. Biocleaning can employ microorganisms or their extracted enzymes to remove inorganic and organic surface deposits such as sulfate crusts, animal glues, biofilms and felt tip marker graffiti. This review seeks to provide updated information on the innovative bioconservation treatments that have been or are being developed.

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“…B. subtilis laboratory strain 168 was used to identify cellular fractions as well as genes and molecules with key roles in inducing precipitation ( Barabesi et al, 2007 ), as found for mollusks ( Falini et al, 1996 ). Characterization of B. subtilis mutants impaired in CaCO 3 precipitation suggested a link between biomineralization, redox reactions of fatty acid metabolism, changes in phospholipids membrane composition and surface properties ( Barabesi et al, 2007 ; Marvasi et al, 2010 , 2016 ; Frandi et al, 2011 ; Perito et al, 2018a ). In Lysinibacillus , CaCO 3 precipitation can modify membrane rigidity by upregulating the branched chain fatty acid synthesis ( Lee and Park, 2019 ).…”

Section: Bccm-based Approaches For Cultural Stone Conservationmentioning

confidence: 99%

Bacterial Calcium Carbonate Mineralization in situ Strategies for Conservation of Stone Artworks: From Cell Components to Microbial Community

2020

Self Cite

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Calcareous stones have been widely used in artworks and buildings by almost all human cultures. Now, more than ever, the increased environmental pollution and global warming are threatening the stone cultural heritage. Weathering due to physical, chemical and biological factors results in monumental calcareous stone deterioration. These agents induce a progressive dissolution of the mineral matrix, increase porosity, and lead to structural weakening. Bacterial Calcium Carbonate Mineralization is a widespread naturally occurring process which in the last decades was proposed as an environmentally friendly tool to protect monumental and ornamental calcareous stones. The advantage of this treatment is that it mimics the natural process responsible for stone formation, producing a mineral product similar to the stone substrate. This mini review highlights the milestones of the biomineralization approaches with focus on in situ stone artworks protection. The strategies explored to date are based on three main approaches: (i) the use of allochthonous and (ii) autochthonous alive cells that, due to the bacterial metabolism, foster biomineralization; (iii) the cell-free approach which uses fractionated cellular components inducing biomineralization. We discuss the challenging aspects of all these techniques, focusing on in situ applications and suggesting perspectives based on recent advances.

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Bacillus subtilis fadB (ysiB) gene encodes an enoyl-CoA hydratase

Cited by 7 publications

References 21 publications

Bacterial molecular machinery in the Martian cryosphere conditions

Bacterial molecular machinery in the Martian cryosphere conditions

Bioconservation of Historic Stone Buildings—An Updated Review

Bacterial Calcium Carbonate Mineralization in situ Strategies for Conservation of Stone Artworks: From Cell Components to Microbial Community

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