Bacillus subtilis from Soybean Food Shows Antimicrobial Activity for Multidrug-Resistant Acinetobacter baumannii by Affecting the adeS Gene

Abstract

Exploring novel antibiotics is necessary for multidrug-resistant pathogenic bacteria. Because the probiotics in soybean food have antimicrobial activities, we investigated their effects on multidrug-resistant . Nineteen multidrug-resistant strains were clinifcally isolated as an experimental group and 11 multidrug-sensitive strains as controls. The growth rates of all bacteria were determined by using the analysis for xCELLigence Real-Time Cell. The combination of antibiotics showed synergistic effects on the strains in the control group but no effect on the strains in the experimental group. Efflux pump gene was absent in all the strains from the control group, whereas it exists in all the strains from the experimental group. Furthermore, all the strains lost multidrug resistance when an inhibitor was used. One strain of probiotics isolated from soybean food showed high antimicrobial activity for multidrug-resistant . The isolated strain belongs to according to 16S RNA analysis. Furthermore, showed multidrug resistance when it was transformed with the gene from whereas the resistant bacteria could be inhibited completely by isolated. Thus, probiotics from soybean food provide potential antibiotics against multidrug-resistant pathogenic bacteria.

Antibacterial Activity of (+) Usnic Acid against Multi Drug Resistant Acinetobacter baumannii from Clinical Isolates

“…Multi drug resistant (MDR) Acinetobacter baumannii (Ab) is considered to be a hospital acquired infection globally. High mortality and prolonged hospital stay are reported in patients infected with MDR Ab (6,7) . The bacteria undergo mutations and resistance mechanisms like efflux pump and enzyme degradation.…”