Bacillus Subtilis HU58 Immobilized in Micropores of Diatomite for Using in Self-healing Concrete

Huynh, Nguyen Ngoc Tri; Phuong, Nghi Mai; Toan, Nguyen Phung Anh; Son, Nguyen Khanh

doi:10.1016/j.proeng.2017.01.385

Cited by 74 publications

(28 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a gram-positive bacteria, Bacillus subtilis natto can survive in the high-alkaline environment of concrete by its ability to form spores (Samanya and Yamauchi 2002;Kawaai et al 2017). Figure 1 demonstrates that the biomineralization mechanism of Bacillus subtilis natto is relatively similar to Bacillus subtilis HU58 (Huynh et al 2017) and other members of the subtilis family. Based on the hydrolysis of urea, bacterial cells become negatively charged, leading to the rapid attraction of surrounding calcium ions.…”

Section: Introductionmentioning

confidence: 91%

“…Therefore, self-healing concrete using biomineralization by bacteria can be a sustainable solution to extend the service life and durability of concrete structures. According to previous studies, limited types of bacteria can be used for MICP, such as Bacillus cohnii (Jonkers 2007), Bacillus pasteurii (Ramachandran et al 2001), Bacillus pseudofirmus (Jonkers and Schlangen 2008), Bacillus subtilis (Huynh et al 2017;Matsushita et al 2010;Rao et al 2013). Each type of bacteria needs proper nutrients for their growth.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Study on Biomineralization using <i>Bacillus Subtilis</i> Natto for Repeatability of Self-Healing Concrete and Strength Improvement

Huynh

Imamoto

Kiyohara

2019

ACT

Self Cite

View full text Add to dashboard Cite

Recent studies in the field of concrete materials show that the early cracking criteria in micro-size can occur as soon as the cement matrix becomes hardened. In many ways, these cracks can become macro-size and opened cracks resulting in significant issues for the durability and appearance of concrete structures as water leakage and corrosion. The technique of self-healing using bacteria has recently received attention for its potential applications. However, the effectiveness and the repeatability of this method over a long period have not been clarified. The information on both the survival and the number of bacteria after healing is limited. This paper aims to improve the self-healing ability and repeatability of concrete when using Bacillus subtilis natto. The experimental studies evaluate the effect of biomineralization with lightweight aggregate as the protecting-carrying vehicle, which can control the release of healing fluid through four cracking-healing cycles. The urease activity and the biomineralization of the bacteria with urea as the main carbon source were assessed and the effect of cracking age on the self-healing capacity, associated with the compressive strength improvement was studied. The results obtained from the optical microscope and SEM/EDS analysis indicated the existence of bacteria CaCO 3 forming in concrete after four healing cycles. During long duration, bacterial concentration in concrete was determined by microscopic counting method. Based on experimental results, the restoration of the compressive strength confirmed the high self-healing ability of concrete when using bacteria in lightweight aggregate.

show abstract

Section: Introductionmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 99%

A Study on Biomineralization using <i>Bacillus Subtilis</i> Natto for Repeatability of Self-Healing Concrete and Strength Improvement

Huynh

Imamoto

Kiyohara

2019

ACT

Self Cite

View full text Add to dashboard Cite

show abstract

“…MICP is a natural biomineralization process that has various applications in environmental remediation [4], improving the strength and stiffness of soil [5], enhancing duration and remediation of concrete [6] and removing calcium in wastewater [7]. However, MICP is a complicated biochemical mechanism with many environmental factors; therefore, the complexity of the MICP procedure creates barriers for applying in the construction field.…”

Section: Introductionmentioning

confidence: 99%

Investigation of Applying Wild Bacillus Species for Sand Stiffening

Pham

Nhi²,

Thủy³

et al. 2019

JST

View full text Add to dashboard Cite

Microbially induced calcium carbonate precipitation is a biomineralization process that has various applications in remediation and restoration of range of applications. In the present study, calcifying bacteria, Bacillus subtilis and Bacillus megaterium isolated from soil (Viet Nam). These species were investigated for sand stiffening using syringe set-up with daily nutrient addition at 7, 14 and 28 days. The stiffened sand samples were tested the physical and chemical properties. The calcium carbonate polymorphs produced by Bacillus subtilis and Bacillus megaterium were analyzed by scanning electron microscopy (SEM), Energy Dispersive Spectroscopy (EDS), X-ray diffraction (XRD). These results show that both of the Bacillus species could make consolidation through calcite precipitation at the alkaline pH rate of 9, increased impact strength of the stiffened sand samples. Furthermore, isolated B. megaterium shows it is potential as bio-sealant to enhance the durability of low energy, green building materials (soil-cement blocks).

show abstract

“…Natural diatomite has been investigated for bacterial adsorption and entrapment into the pores with final insertion made in the form of pellets. Addition of pellets enhanced compressive strength but lowered the permeability of the resultant matrix [23]. Ceramsite (sand) was also used as a carrier, but microbes and nutrients were immobilized separately in that case, eventually providing an accomplished repair rate of 87.5% [24].…”

Section: Introductionmentioning

confidence: 99%

Bioimmobilized Limestone Powder for Autonomous Healing of Cementitious Systems: A Feasibility Study

Shaheen

Khushnood

Din

2018

Advances in Materials Science and Engineering

View full text Add to dashboard Cite

For preserving concrete structures and hindering ingress of chemicals through cracks and fissures, repair is inevitable. Microbial calcite precipitation is an intrinsic approach for crack rectification and emulating way of sustainability for reducing anthropogenic greenhouse gases (GHGs) along with conserving the natural resources. In this study,Bacillus subtilisstrain is applied for intrinsic repair of concrete’s cracks because of its high pH endurance and capability of sporulation. For prolonged survival of microorganisms, immobilization technique was employed.B. subtiliswas immobilized through limestone powder (LSP) before adding into cement matrix. Self-healing proficiency ofB. subtiliswas deliberated in terms of mechanical strength regain after cracking at 3, 7, 14, and 28 days. To examine the microstructure and characterization of healing precipitate, micrographical (field emission scanning electron microscopy), chemical (energy dispersive X-ray), and thermal (thermogravimetric analysis) analyses were performed after the healing period of 28 days. The results revealed evident signs of calcite precipitation in nano-/microcracks subsequent to microbial activity. Furthermore, immobilized LSP improved the compressive strength of the analyzed formulations.

show abstract

Bacillus Subtilis HU58 Immobilized in Micropores of Diatomite for Using in Self-healing Concrete

Cited by 74 publications

References 5 publications

A Study on Biomineralization using <i>Bacillus Subtilis</i> Natto for Repeatability of Self-Healing Concrete and Strength Improvement

A Study on Biomineralization using <i>Bacillus Subtilis</i> Natto for Repeatability of Self-Healing Concrete and Strength Improvement

Investigation of Applying Wild Bacillus Species for Sand Stiffening

Bioimmobilized Limestone Powder for Autonomous Healing of Cementitious Systems: A Feasibility Study

Contact Info

Product

Resources

About