Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD+-dependent scyllo-inositol dehydrogenase

Kang, Dong-Min; Michon, C.; Morinaga, Tetsuro; Tanaka, Kosei; Takenaka, Shinji; Ishikawa, Shu; Yoshida, Kenichi

doi:10.1186/s12866-017-1065-8

Cited by 7 publications

(23 citation statements)

References 30 publications

(57 reference statements)

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“…There is extensive research on the biochemical pathway and the regulation of bacterial inositol catabolism in Bacillus subtilis. It has been reported that B. subtilis possesses a complete set of iol genes required for its inositol catabolism; including the iolABCDEFGHIJ operon, the iolRS operon, iolQ, iolT, iolU, iolW, and iolX [1,2,3,4,5,6].…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, a recent study demonstrated that in B. subtilis, an additional repressor encoded by iolQ that belongs to the LacI family regulates iolX, encoding an NAD + -dependent scyllo-inositol dehydrogenase [3], although the mechanisms underlying the regulation of iolU and iolW, either of which encodes an NADP + -dependent scyllo-inositol dehydrogenase [5,6], have not been elucidated as they appeared to be almost constitutive. Genetic evidence suggests that scyllo-inositol and myo-inositol could be the intracellular inducers for IolQ; however, both failed to antagonize its DNA binding activity in vitro [3].…”

Section: Introductionmentioning

confidence: 99%

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Identification of a Repressor for the Two iol Operons Required for Inositol Catabolism in Geobacillus Kaustophilus

Yoshida

Shirae

Nishimura

et al. 2020

Preprint

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BackgroundGeobacillus kaustophilus HTA426, a thermophilic Gram-positive bacterium, grows on inositol as its sole carbon source, and an iol gene cluster required for inositol catabolism has been postulated with reference to the iol genes in Bacillus subtilis. The iol gene cluster consists of two tandem operons induced in the presence of inositol; however, the mechanism underlying the induction remains unclear. B. subtilis iolQ is known to be involved in the regulation of iolX encoding a scyllo-inositol dehydrogenase, and its homolog in HTA426 was found two genes upstream of the first gene (gk1899) of the iol gene cluster and termed as iolQ in G. kaustophilus.ResultsWhen iolQ was inactivated, not only the myo-inositol dehydrogenase activity in the cell due to the expression of gk1899 but also the transcription of the two iol operons became constitutive. IolQ was produced and purified as a C-terminal His-tag fusion in Escherichia coli and subjected to the in vitro gel mobility shift assay to examine its DNA binding property. It was observed that IolQ bound to the DNA fragments containing each of the two iol promoter regions, and its DNA binding was antagonized by myo-inositol. Moreover, DNase I footprint analyses were conducted to determine the two binding sites of IolQ within each of the iol promoter regions. By comparing the sequences of the binding sites, a consensus sequence for IolQ binding was deduced to be a palindrome of 5′-RGWAAGCGCTTSCY-3′ (where R = A or G, W = A or T, S = G or C, and Y = C or T).ConclusionIolQ functions as a transcriptional repressor regulating the induction of the two iol operons responding to myo-inositol.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of a Repressor for the Two iol Operons Required for Inositol Catabolism in Geobacillus Kaustophilus

Yoshida

Shirae

Nishimura

et al. 2020

Preprint

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“…scyllo-Inosose is further degraded, enabling it to enter glycolysis and the citric acid cycle following successive reactions catalyzed by the enzymes IolE, IolD, IolB, IolC, IolJ, and IolA. In addition, both IolF and IolT function in transporting inositol into the cell, while IolR and IolQ act as repressors regulating transcription of the iol genes [10][11][12][13][14] . An additional inositol dehydrogenase, IolW, is a key enzyme that enables B. subtilis to produce scyllo-inositol by specifically reducing scyllo-inosose into scyllo-inositol, coupled with the oxidation of NADPH ( Fig.…”

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confidence: 99%

“…In this case, iolG, iolW, and iolT were simultaneously overexpressed in a strain that lacked all of the other iol genes. In addition, a heterologous nicotinamide nucleotide transhydrogenase capable of regenerating NADPH was introduced, since IolW requires NADPH to reduce scyllo-inosose into scyllo-inositol 14 . The bioconversion is efficient enough to enable a high productivity, but the scylloinositol produced can never be cheaper than the starting material, myo-inositol.…”

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confidence: 99%

“…Within the genome of B. subtilis, there is no gene likely to encode MI1PS, but there is an intrinsic yktC gene encoding functional inositol monophosphatase 22 . Therefore, we speculated that simply expressing M. tuberculosis ino1 in B. subtilis may enable myo-inositol biosynthesis, and this myo-inositol can be introduced to a previously established cell factory platform that converts myo-inositol into scyllo-inositol 14 , enabling the production of scyllo-inositol from glucose in a single bacterial cell factory (Fig. 1).…”

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A bacterial cell factory converting glucose into scyllo-inositol, a therapeutic agent for Alzheimer’s disease

et al. 2020

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A rare stereoisomer of inositol, scyllo-inositol, is a therapeutic agent that has shown potential efficacy in preventing Alzheimer's disease. Mycobacterium tuberculosis ino1 encoding myoinositol-1-phosphate (MI1P) synthase (MI1PS) was introduced into Bacillus subtilis to convert glucose-6-phosphate (G6P) into MI1P. We found that inactivation of pbuE elevated intracellular concentrations of NAD + •NADH as an essential cofactor of MI1PS and was required to activate MI1PS. MI1P thus produced was dephosphorylated into myo-inositol by an intrinsic inositol monophosphatase, YktC, which was subsequently isomerized into scyllo-inositol via a previously established artificial pathway involving two inositol dehydrogenases, IolG and IolW. In addition, both glcP and glcK were overexpressed to feed more G6P and accelerate scyllo-inositol production. Consequently, a B. subtilis cell factory was demonstrated to produce 2 g L −1 scyllo-inositol from 20 g L −1 glucose. This cell factory provides an inexpensive way to produce scyllo-inositol, which will help us to challenge the growing problem of Alzheimer's disease in our aging society.

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Roseibium algae sp. nov., isolated from a marine alga (Grateloupia sp.)

Fan,

Liu,

Wang

et al. 2024

International Journal of Systematic and Evolutionary Microbiology

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A novel Gram-stain-negative, rod-shaped, non-spore-forming, aerobic, motile bacterium with a single polar or subpolar flagellum, designated strain H3510T, was isolated from marine alga collected on sea shore of Yantai, PR China. The organism grew optimally at 28 °C and pH 7.0 and in presence of 3.0 % (w/v) NaCl. The strain exhibited positive catalase activity but negative oxidase and nitrate reduction activities. The predominant cellular fatty acids were C18 : 1 ω7c and/or C18 : 1 ω6c, 11-methyl C18 : 1 ω7c, and C16 : 0. Additionally, the major polar lipids were phosphatidylglycerol, phosphatidylmonomethylethanolamine, diphosphatidylglycerol, and phosphatidylethanolamine; the respiratory quinone was ubiquinone 10 (Q-10). The genomic DNA G+C content of strain H3510T was 54.2%. The novel strain showed the closest relationship with Roseibium polysiphoniae KMM 9699T with 98.2 % 16S rRNA gene sequence similarity. The calculated values for average nucleotide identity and DNA–DNA hybridization between strain H3510T and the phylogenetically related Roseibium species were in the range of 71.3–74.9 % and 13.7–19.9 %, respectively. Based on polyphasic analyses, strain H3510T was identified as representing a novel species of the genus Roseibium, for which the name Roseibium algae sp. nov. is proposed. The type strain is H3510T (=KCTC 8206T=MCCC 1K04325T). The heterologously expressed inositol 2-dehydrogenase gene from strain H3510T displayed high oxidation activity on myo-inositol and showed potential in the production of rare stereoisomers of inositol, such as scyllo-inositol.

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Bacillus subtilis IolQ (DegA) is a transcriptional repressor of iolX encoding NAD+-dependent scyllo-inositol dehydrogenase

Cited by 7 publications

References 30 publications

Identification of a Repressor for the Two iol Operons Required for Inositol Catabolism in Geobacillus Kaustophilus

Identification of a Repressor for the Two iol Operons Required for Inositol Catabolism in Geobacillus Kaustophilus

A bacterial cell factory converting glucose into scyllo-inositol, a therapeutic agent for Alzheimer’s disease

Roseibium algae sp. nov., isolated from a marine alga (Grateloupia sp.)

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