Bacillus subtilis transcription regulator, Spo0A, decreases alkaline phosphatase levels induced by phosphate starvation

Jensen, K; Sharkova, E. V.; Duggan, M. F.; Ying, Qi; Koide, Akiko; Hoch, James A.; Hulett, F. Marion

doi:10.1128/jb.175.12.3749-3756.1993

Cited by 25 publications

(36 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…APase was induced in response to phosphate starvation in the wild-type (Fig. 1) and threefold hyperinduced in the spaUA mutant, as Psi proteins in B. szrbtilis described by Jensen et al (1993). As expected, APase was not induced in thepboR mutant.…”

Section: Cell Growth Methionine Incorporation and Synthesis Of Apasementioning

confidence: 52%

Phosphate-starvation-inducible proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study

et al. 1996

View full text Add to dashboard Cite

A two-dimensional (2-D) gel electrophoresis study of Bacillus subtilis strain 168 identified 20 proteins that are strongly induced in response to phosphate starvation. The induction of nine of these phosphate-starvation-induced (Psi) proteins was dependent on a functional PhoR protein. PhoR is the histidine sensor-kinase component of a phosphate-concentration-sensing twocomponent regulatory system which, together with its partner response regulator PhoP, controls the expression of genes in the Pho regulon. Genes encoding PhoR-dependent Psi proteins are therefore likely to be members of the Pho regulon. SpoOA -P, the response regulator of the signal transduction pathway required for the induction of sporulation, has previously been shown to negatively affect the induction of the Pho regulon by repressing the phoPqahoR operon. The induction pattern of some PhoR-dependent Psi proteins was altered in a spoOA mutant such that their synthesis continued for longer than was found with the wild-type. The most abundant Psi protein, Psil-3, was characterized by N-terminal sequencing of internal peptide fragments and shown to have a high similarity to an Escherichia d i protein which is involved in phosphate uptake during phosphate starvation.

show abstract

Section: Cell Growth Methionine Incorporation and Synthesis Of Apasementioning

confidence: 52%

Phosphate-starvation-inducible proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study

et al. 1996

View full text Add to dashboard Cite

show abstract

“…A similar pattern was observed after changing to phosphate limitation in the presence of IPTG ; the altered cell wall composition was enhanced, although there was a reduction in APase activity. Phosphate starvation is known to trigger sporulation and the lower induced APase activity could be due to the previously observed influence of SpoOA on the expression of phoA (Hulett et al, 1994a;Jensen et al, 1993).…”

Section: Influence Of Phor On the Synthesis Of Anionic Polymersmentioning

confidence: 99%

“…Similarities between the E. coli and B. subtilis components and the genomic organization of their genes suggest that their Pho regulons function in a similar manner (Seki et al, 1987(Seki et al, , 1988. If cultures continue to experience growth-limiting phosphate stress, a second response regulator, SpoOA -P, terminates the phosphate response and initiates sporulation (Jensen et al, 1993). Recently, a third signal-transduction system, ResD/ResE, has been shown to be required for full induction of the Pho regulon (Hulett, 1995).…”

Section: Introductionmentioning

confidence: 99%

Influence of Bacillus subtilis phoR on cell wall anionic polymers

Müler

Merad

et al. 1997

Microbiology

View full text Add to dashboard Cite

In Bacillus subtilis the Pho regulon is controlled by a sensor and regulator protein pair, PhoR and PhoP, that respond to phosphate concentrations. To facilitate studies of the Pho regulon, a strain with an altered PhoR protein was isolated by in vitro mutagenesis. The mutation in this strain (phoR12) leads to the production of a PhoR sensor kinase that, unlike the wild-type, is functionally active in phosphate-replete conditions. The lesion in phoRl2 was shown to be a single base change that results in an Arg to Ser substitution in a region of PhoR that is highly conserved in histidine sensor kinases. While a phoR-negative mutant was unable to induce the synthesis of cell wall teichuronic acid under phosphate-limited conditions, the phoR72 mutant showed a relative increase in teichuronic acid and a decrease in teichoic acid, even under phosphate-replete conditions. The latter suggests that some or all of the genes required for teichuronic acid synthesis are members of the Pho reg ulon.Keywords : phosphate stress, histidine sensor kinase, response regulator, alkaline phosphatase, signal transduction INTRODUCTIONPhosphorus is a major growth-limiting substrate in natural environments such as soil (Hulett, 1993). When the growth of Bacillus subtilis becomes limited by the availability of phosphorus, a programmed series of responses is induced that can ultimately lead to sporulation. Among the earliest of these responses are processes that reduce the cell's requirement for phosphorus, increase its affinity for the uptake of phosphate and lead to the production of extracellular hydrolytic enzymes that allow the cell to recover inorganic phosphate from organic sources such as teichoic acids (Grant, 1979;Eymann et al., 1996). If these conservation and scavenging mechanisms are effective, the developmental pathway leading to sporulation is reversed and the organism resumes vegetative growth. B. subtilis responds to phosphate stress by the coordinated induction of approximately 20 genes (Eymann et al., 1996). Prominent among these are genes of the Pho regulon (Seki et al., 1987;Hulett, 1993; Hulett et al., 1994a, b) , whose products include alkaline phosphatases (APase) , alkaline phosphodiesterase (APDase) , a highaffinity phosphate transporter and, as reported in this paper, proteins involved in teichuronic acid synthesis. a two-component, environment-sensing, signal-transduction system (Seki et al., 1987(Seki et al., ,1988. The components of this system, PhoP and PhoR, are equivalent, respectively, to the PhoB and PhoR proteins of Escherichia coli (Tommassen etal., 1982;Makino et al., 1985Makino et al., ,1986. In E. coli, PhoR is a membrane-spanning sensor protein with histidine protein kinase (HPKase) and phosphatase (PTase) activities Scholten & Tommassen, 1993). Activation at low phosphate concentrations leads to the production of PhoR -P which, in turn, acts as a substrate for the activation of the response regulator PhoB, required for the induction of the Pho regulon (Stock et al., 1989;Parkinson & Kofoid, 199...

show abstract

“…PhoR is a membrane-spanning sensor protein with histidine protein kinase (HPKase) activity that, at low phosphate concentrations, activates its cognate response regulator, PhoP. Two other signal transduction pathways interact with PhoP\PhoR : Spo0A" P, the activated product of the phosphorelay (Hoch, 1998) that is responsible for the induction of sporulation, terminates the phosphate response (Jensen et al, 1993), while ResD\ResE are required for the full induction of the Pho regulon (Hulett, 1995) by modulating the expression of the phoPR operon. In this paper we identify a large operon (tua) required for the synthesis of teichuronic acid and confirm that the influence of PhoR on teichuronic acid biosynthesis is due to its effect on controlling the transcriptional activity of this operon.…”

Section: Abbreviationsmentioning

confidence: 99%

Transcriptional analysis of the Bacillus subtilis teichuronic acid operon

Lahooti

Harwood

1999

Microbiology

View full text Add to dashboard Cite

The cell walls of Gram-positive bacteria consist primarily of a macromolecular matrix comprising similar amounts of peptidoglycan and covalently attached anionic polymers. Under most growth conditions the anionic polymers of Bacillus subtilis are principally teichoic acids ; in strain 168 these include a polyglycerol teichoic acid and a glucose/galactosamine-containing teichoic acid. However, when cultures are subjected to phosphate stress the bacterium induces a complex series of responses, one of which is the replacement of at least part of the wall teichoic acid with teichuronic acid, a non-phosphatecontaining anionic polymer. In this paper the construction of a transcriptional reporter strain that facilitates the monitoring of the promoter region upstream of the tua operon involved in teichuronic acid synthesis and its controlled expression are reported. The expression of the tua operon was monitored in both phosphate-starved, non-growing batch cultures and phosphate-limited continuous cultures. We show that the transcription of the operon correlates well with the anionic polymer composition of the cell walls.

show abstract

Bacillus subtilis transcription regulator, Spo0A, decreases alkaline phosphatase levels induced by phosphate starvation

Cited by 25 publications

References 29 publications

Phosphate-starvation-inducible proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study

Phosphate-starvation-inducible proteins in Bacillus subtilis: a two-dimensional gel electrophoresis study

Influence of Bacillus subtilis phoR on cell wall anionic polymers

Transcriptional analysis of the Bacillus subtilis teichuronic acid operon

Contact Info

Product

Resources

About