Bacillus thermoglucosidasius sp. nov., a New Species of Obligately Thermophilic Bacilli

Suzuki, Yuzuru; Kishigami, Takashi; Inoue, Kiyoshi; Mizoguchi, Yasuhiro; Eto, Nobuyuki; Takagi, Makoto; Abe, S.

doi:10.1016/s0723-2020(83)80006-x

Cited by 100 publications

(46 citation statements)

References 11 publications

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“…Symbols: +, growth/activity observed; 2, no growth/activity observed; W, weak growth; ND, not determined; V, variable within group. Data are from the present study (G. gargensis) or from Logan & Berkeley (1984), Claus & Berkeley (1986) and Priest et al (1988) (G. stearothermophilus); Golovacheva et al (1975), Nazina et al (2001) and data from the present study (G. thermocatenulatus); Suzuki et al (1983) (2000) and data from the present study (B. vulcani=G. vulcani).…”

Section: S Rrna Gene Sequence Analysismentioning

confidence: 66%

“…Geobacillus thermodenitrificans, Geobacillus subterraneus, Geobacillus uzenensis, Geobacillus caldoxylosilyticus and Geobacillus toebii (Logan & Berkeley, 1984;Claus & Berkeley, 1986;Golovacheva et al, 1975;Suzuki et al, 1983;Priest et al, 1988;White et al, 1993;Zarilla & Perry, 1987;Manachini et al, 2000;Ahmad et al, 2000;Nazina et al, 2001;Fortina et al, 2001a;Sung et al, 2002). Many thermophilic bacilli that have not yet been included in recognized species ('Bacillus caldolyticus', 'Bacillus caldotenax', 'Bacillus caldovelox' and 'Geobacillus uralicus'), and the recognized species Bacillus thermantarcticus, Saccharococcus thermophilus and Bacillus vulcani belong to genetic group 5, which is close to the genus Geobacillus both phenotypically and phylogenetically (Ash et al, 1991;Rainey et al, 1994;Caccamo et al, 2000;Heinen & Heinen, 1972;Rainey & Stackebrandt, 1993;Nystrand, 1984;Nicolaus et al, 1996;Popova et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

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Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov.

Назина

Лебедева

Полтараус

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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A novel thermophilic spore-forming strain, GaT, was isolated from the Garga hot spring located in the northern part of the Transbaikal region (Russia). Strain GaT was found to be an aerobic, Gram-positive, rod-shaped, thermophilic (optimum growth temperature is 60–65 °C), chemo-organotrophic bacterium that grows on various sugars, carboxylic acids and hydrocarbons. The G+C content of its DNA is 52·9 mol%. The 16S rRNA gene sequence similarity data show that strain GaT is closely related to members of the genus Geobacillus. Relevant chemotaxonomic data (in particular, the major fatty acid profile of strain GaT, which includes iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0 acids) support the assignment of this strain to the genus Geobacillus. The physiological, biochemical and DNA–DNA hybridization studies of strain GaT showed that it differs both genotypically and phenotypically from the recognized Geobacillus species. Based on these data, strain GaT belongs to a novel species, Geobacillus gargensis sp. nov. (type strain, GaT=VKM B-2300T=DSM 15378T). The analysis of the phenotypic characteristics (additional to those given in the original description) of the type strain of Bacillus vulcani (DSM 13174T) showed that they are very similar to the major phenotypic characteristics of the genus Geobacillus. The low DNA–DNA reassociation values of strain DSM 13174T with various species of this genus (from 38 to 54 %) clearly demonstrate a sufficient genomic distinction of this strain and its taxonomic status as a species. The physiological characteristics, phylogenetic position and DNA–DNA reassociation values of B. vulcani allow this species to be reclassified as Geobacillus vulcani comb. nov. The main properties that differentiate G. vulcani from the other species of the genus are its ability to produce acids from glycerol, lactose and ribose.

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Section: S Rrna Gene Sequence Analysismentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov.

Назина

Лебедева

Полтараус

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

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“…AK1 was the bacteria most closely related to the isolate for which 16S rRNA sequence data is available. On the other hand, the phylogenetically most closely related to Bacillus sp AK1 species is B. thermoglucosidasius (23). However, the isolate showed that levels of similarity to this organism ranged from 90% to 91%.…”

Section: Discussionmentioning

confidence: 96%

Isolation, properties and kinetics of growth of a thermophilic Bacillus

Souza¹,

Martins²

2001

Braz. J. Microbiol.

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A strictly aerobic, thermophilic, spore-forming bacteria was isolated from a soil sample collected in Campos dos Goytacazes City, Rio de Janeiro, Brazil. The cells of this organism were Gram-positive, catalase positive, actively motile, 1.2 µm wide and 5.3 µm long. Growth occurred at pH values ranging from 6.5 to 9.0, and optimum growth occurred at about pH 7.0. The optimum temperature for growth was around 55ºC, and the upper temperature limit for growth was around 70ºC. Yeast extract stimulated growth on glucose and was obligately required at supraoptimal temperatures (65ºC). The results of 16S rRNA sequence comparisons indicated that the isolate was closely related to Bacillus caldoxylolyticus and Bacillus sp strain AK1, and these three organisms exhibited levels of ribossomal DNA sequence homology of 94%.

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“…No growth was detected in the presence of 3 % NaCl. The isolates reduced nitrate to nitrite and strains TS2A, TS24A and TS33B also showed the ability to reduce Suzuki et al (1983) (G. thermoglucosidasius), Logan & Berkeley (1984) and Claus & Berkeley (1986) (G. stearothermophilus) and Manachini et al (2000) (G. thermodenitrificans). All isolates and reference strains were positive for the Gram reaction, catalase and utilization of glucose, fructose, maltose, trehalose, mannose and sucrose.…”

Section: Morphology and Biochemical Propertiesmentioning

confidence: 99%

Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov.

Fortina¹,

Mora²,

Schümann³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

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A polyphasic study was performed on five thermophilic strains belonging to the genus Bacillus, isolated from soil of different geographical areas. 16S rRNA gene sequence analysis placed these isolates in RNA group 5, with Saccharococcus caldoxylosilyticus and [Bacillus] thermoglucosidasius being the closest phylogenetic neighbours. The type species of Saccharococcus, Saccharococcus thermophilus, was only moderately related to these two species and the novel isolates. DNA-DNA hybridization studies and comparison of morphological, chemotaxonomic and phenotypic features supported the close relationship between the novel isolates and Saccharococcus caldoxylosilyticus. These data justify the reclassification of Saccharococcus caldoxylosilyticus. Following the transfer of the validly described Bacillus species of group 5 into the genus Geobacillus, the reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus comb. nov. is proposed. This species can be distinguished genomically from Geobacillus thermoglucosidasius, Geobacillus stearothermophilus, Geobacillus thermodenitrificans and Saccharococcus thermophilus by a specific PCR-RFLP assay targeting the 16S rDNA.

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Bacillus thermoglucosidasius sp. nov., a New Species of Obligately Thermophilic Bacilli

Cited by 100 publications

References 11 publications

Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov.

Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov.

Isolation, properties and kinetics of growth of a thermophilic Bacillus

Reclassification of Saccharococcus caldoxylosilyticus as Geobacillus caldoxylosilyticus (Ahmad et al. 2000) comb. nov.

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