The main purpose of this study is to examine how the use of management tools supports the readiness of manufacturing organizations for the implementation of Industry 4.0. The originality of the research is reflected in the exploration of the relationship between the use of the selected well-known management tools and their readiness for the implementation of Industry 4.0, which was assessed using a combination of two models—one developed by the National Academy of Science and Engineering (Acatech) and the other by the University of Warwick. The relationship was assessed by applying structural equation modeling techniques to a data set of 323 responses from employees in manufacturing organizations. The results show that the use of six sigma, total quality management, radio frequency identification, a balanced scorecard, rapid prototyping, customer segmentation, mission and vision statements, and digital transformation is positively associated with Industry 4.0 readiness. Inversely, outsourcing and strategic planning are negatively associated with Industry 4.0 readiness, while lean manufacturing, which is often emphasized as the cornerstone of Industry 4.0 implementation, is not associated with Industry 4.0 readiness in our study. These findings can help organizations to understand how to consider and measure readiness for the implementation of Industry 4.0 more comprehensively and present guidelines on how the use of management tools in manufacturing organizations can foster their implementation of Industry 4.0 principles.