Background pathology of the ovary in a laboratory population of zebrafish Danio rerio

Rossteuscher, Stefanie; Schmidt‐Posthaus, Heike; Schäfers, Christoph; Teigeler, Matthias; Segner, Helmut

doi:10.3354/dao01893

Cited by 15 publications

(7 citation statements)

References 7 publications

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Section: Introductionmentioning

confidence: 99%

“…Although ovaries with this condition may be infected with mycobacteria (Whipps et al 2008), many cases are not associated with readily detectable infectious agents (Rossteuscher et al 2008). In one zebrafish study, increased atresia (degeneration) of mature eggs was statistically correlated with the occurrence of egg debris (Rossteucher et al 2008). The authors of that investigation concluded that the associated inflammatory changes could be caused either by primary inflammation followed by increased atresia, or conversely, by a secondary inflammatory response to the presence of egg debris.…”

Section: Introductionmentioning

confidence: 99%

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Documented and Potential Research Impacts of Subclinical Diseases in Zebrafish

Kent

Harper²,

Wolf³

2012

ILAR Journal

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The zebrafish (Danio rerio) has become a very important animal model in biomedical research. In contrast to other models, such as mice, there has been relatively little documentation or control of subclinical disease in zebrafish research facilities. Several infectious and non-infectious conditions are consistently detected by histopathology in apparently healthy D. rerio. The most commonly observed infectious agent in zebrafish is Pseudoloma neurophilia, which is a microsporidian organism that targets the central nervous system, peripheral nerves, and occasionally other tissues. Mycobacteriosis, caused by M. chelonae and other species, is also a frequent finding. Less commonly encountered agents include Pseudocapillaria tomentosa, which can cause extensive proliferative enteritis, and a myxozoan (Myxidium sp.) that inhabits the urinary tract, but appears to cause few if any pathological changes. Non-infectious diseases that are often clinically unapparent in zebrafish include hepatic megalocytosis, bile and pancreatic ductal proliferation, and neoplasms of the ultimobranchial gland, gastrointestinal tract, and testis. To date there is little information on the degree to which these conditions may impact research in subclinically affected fish, but there is reason to believe that they should be considered as potentially significant causes of non-protocol variation in experiments. Therefore, it is imperative that research facilities monitor their stocks for the presence of these occult diseases, and be aware of their existence when interpreting study results. Furthermore, for underlying disease conditions that cannot be readily eradicated, it is essential to determine the physiological and immunological changes that they elicit in zebrafish. Understanding the cause, modes of transmission, and distribution of the pathogens would provide useful information for the development of control and prevention strategies.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Documented and Potential Research Impacts of Subclinical Diseases in Zebrafish

Kent

Harper²,

Wolf³

2012

ILAR Journal

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“…12,15 In a report of zebrafish ovarian pathology, EAI was found in 42% of 59 3-mo-old fish. 22 Although EAI was higher in our fish (93%), this may be partly attributable to their Table 3. Histologic changes for each MS222 storage condition for acute (24 h) and chronic (2 wk) timepoints.…”

Section: Discussionmentioning

confidence: 66%

The Stability and Efficacy of Tricaine Methanesulfonate (MS222) Solution After Long-Term Storage

Katz¹,

Chu²,

Casey³

et al. 2020

j am assoc lab anim sci

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Tricaine methanesulfonate (MS222) is widely used for the anesthesia and euthanasia of laboratory zebrafish. Fresh solutions have been recommended for each use; however, researchers often mix and store concentrated stock solutions for convenience and to reduce occupational exposure and environmental waste. While this is common practice, published guidelines are often inconsistent. Thus, the objective of this study was to evaluate the stability and anesthetic efficacy of MS222 after long-term storage and to develop specific storage parameters. Stock solutions (100 mg/mL MS222) were mixed and stored in amber jars at 4 °C and -20 °C for 2- and 6-mo. Stability of the solutions was analyzed using liquid chromatography-ion trapmass spectrometry and compared with fresh MS222. Fifty adult (30 male, 20 female) wildtype AB zebrafish (Danio rerio) wererandomly anesthetized with 150 mg/L of one of the following MS222 solutions to evaluate anesthetic efficacy: 1) freshly prepared(0m); 2) 2 mo at 4 °C (2m4); 3) 2 mo at -20 °C (2m-20); 4) 6 mo at 4 °C (6m4); 5) 6 mo at -20 °C (6m-20). Time to cessation of swimming, loss of equilibrium, lack of response to von Frey (VF) stimulation, return of equilibrium, and resumption of swimming were compared between groups. Two fish from each group were euthanized at 24-h and 2-wk after anesthesia, and histopathology was performed. All solutions were determined to be stable under all storage conditions. No clinically significant differences were observed between the fresh and stored stock groups during anesthetic testing. No evidence ofanesthetic-related histologic changes were noted in the gills, skin, kidneys, muscle, and central nervous system. Hepatic megalocytosis and a reduction in hepatic vacuolation were seen to varying degrees across all groups, but did not follow a treatment-related trend. Therefore, 100 mg/mL solutions of MS222 can be stored in amber jars at 4 °C or -20 °C for 6 mo and still used to effectively anesthetize zebrafish.

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“…Also, fish exposed to this high concentration experienced an increase in oocyte atresia. Follicular atresia can be a natural degenerative process , often occurring spontaneously in zebrafish , as evidenced by the common incidence of the finding in control fish . It can also be indicative of having reached a systemic level of toxicity or stress .…”

Section: Discussionmentioning

confidence: 99%

Short‐term fish reproduction assays with methyl tertiary butyl ether with zebrafish and fathead minnow: Implications for evaluation of potential for endocrine activity

Mihaich¹,

Erler

et al. 2015

Enviro Toxic and Chemistry

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The authors report on short-term fish reproduction assays in zebrafish and fathead minnow conducted to examine the potential for methyl tertiary butyl ether (MTBE) to cause effects on the endocrine system. Both studies were performed under good laboratory practice and in accordance with Organisation for Economic Co-operation and Development and US Environmental Protection Agency test guidelines. The results of the first study demonstrated that exposure to a high test concentration (147 mg/L) of MTBE impaired reproductive output of female zebrafish, evident by a reduction in fecundity. Based on the endpoints evaluated in the present study however, there was no supporting evidence to indicate that this effect was caused by disruption of or interaction with the endocrine system. In the second study, fathead minnows exposed to a wider but lower range of test concentrations showed no effects on any reproductive parameter of male or female fish, at the maximum recommended testing concentration of 100 mg/L (62 mg/L measured). The results of these 2 guideline studies indicate that MTBE does not interact with the hypothalamic-pituitary-gonadal axis of zebrafish or fathead minnow.

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Background pathology of the ovary in a laboratory population of zebrafish Danio rerio

Cited by 15 publications

References 7 publications

Documented and Potential Research Impacts of Subclinical Diseases in Zebrafish

Documented and Potential Research Impacts of Subclinical Diseases in Zebrafish

The Stability and Efficacy of Tricaine Methanesulfonate (MS222) Solution After Long-Term Storage

Short‐term fish reproduction assays with methyl tertiary butyl ether with zebrafish and fathead minnow: Implications for evaluation of potential for endocrine activity

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