Bacteremia in a human caused by an XDR strain of Pseudomonas fulva

Uddin, Fakhur; Roulston, Kerry; McHugh, Timothy D.; Khan, Taseer Ahmed; Sohail, Muhammad

doi:10.3855/jidc.10326

Cited by 8 publications

(4 citation statements)

References 15 publications

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“…Findings further highlight the clinical importance of emerging resistance in non-traditional GNB such as Citrobacter spp., Non- baumannii Acinetobacter spp., Non- aeruginosa Pseudomonas spp. and Psychrobacter spp., where high levels of MDR and ESBL production detected in this study correlate with reports in the medical literature [ 20 , 21 , 22 , 23 , 24 ]. The Proteus species are additionally emerging as important opportunistic pathogens, where they are now the third most-common etiological factor of UTIs following Escherichia coli and Klebsiella pneumoniae [ 25 ], prompting the WHO to list these species among the Enterobacteriaceae of critical priority.…”

Section: Discussionsupporting

confidence: 91%

Effective Antimicrobial Solutions for Eradicating Multi-Resistant and β-Lactamase-Producing Nosocomial Gram-Negative Pathogens

Meade

Savage²,

Garvey

2021

Antibiotics

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Antimicrobial resistance (AMR) remains one of the greatest public health-perturbing crises of the 21st century, where species have evolved a myriad of defence strategies to resist conventional therapy. The production of extended-spectrum β-lactamase (ESBL), AmpC and carbapenemases in Gram-negative bacteria (GNB) is one such mechanism that currently poses a significant threat to the continuity of first-line and last-line β-lactam agents, where multi-drug-resistant GNB currently warrant a pandemic on their own merit. The World Health Organisation (WHO) has long recognised the need for an improved and coordinated global effort to contain these pathogens, where two factors in particular, international travel and exposure to antimicrobials, play an important role in the emergence and dissemination of antibiotic-resistant genes. Studies described herein assess the resistance patterns of isolated nosocomial pathogens, where levels of resistance were detected using recognised in vitro methods. Additionally, studies conducted extensively investigated alternative biocide (namely peracetic acid, triameen and benzalkonium chloride) and therapeutic options (specifically 1,10-phenanthroline-5,6-dione), where the levels of induced endotoxin from E. coli were also studied for the latter. Antibiotic susceptibility testing revealed there was a significant association between multi-drug resistance and ESBL production, where the WHO critical-priority pathogens, namely E. coli, K. pneumoniae, A. baumannii and P. aeruginosa, exhibited among the greatest levels of multi-drug resistance. Novel compound 1,10-phenanthroline-5,6-dione (phendione) shows promising antimicrobial activity, with MICs determined for all bacterial species, where levels of induced endotoxin varied depending on the concentration used. Tested biocide agents show potential to act as intermediate-level disinfectants in hospital settings, where all tested clinical isolates were susceptible to treatment.

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Section: Discussionsupporting

confidence: 91%

Effective Antimicrobial Solutions for Eradicating Multi-Resistant and β-Lactamase-Producing Nosocomial Gram-Negative Pathogens

Meade

Savage²,

Garvey

2021

Antibiotics

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“…Pseudomonas species other than P. aeruginosa were commonly considered as saprophytes, but recently these have been considered as opportunistic human pathogens, and outbreaks have also been reported from such species [1,2]. P. balearica is mainly found in marine and freshwater, salt marshes, and wastewater [3][4].…”

Section: Introductionmentioning

confidence: 99%

Verona integron encoded metallo Beta lactamase (VIM) and Vietnam extended spectrum Beta lactamase (VEB) producing Pseudomonas balearica from a clinical specimen

et al. 2022

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Pseudomonas balearica, a saprophyte found in marshy and marine habitats, is not routinely differentiated from P. aeruginosa and P. stutzeri using automated systems and hence has not been reported from clinical samples. This study describes the identification of P. balearica using MALDI-TOF-MS and 16S rDNA sequence from a patient admitted to an intensive care unit (I.C.U.). The isolate was found to be Verona integron-mediated Metallo-Beta-lactamase (V.I.M.), and Vietnam extended-spectrum Beta-lactamase (V.E.B.) producer and resistant to Ceftriaxone, Imipenem, and Tobramycin. P. balearica can be a source for horizontal transfer of blaVEB and blaVIM. Its pathogenesis has yet to be understood. Continue...

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“…Interestingly, P. fulva was also involved in the intimate bacterial–fungal interactions that stimulate the effective production of an anticancer therapy agent (hypocrellins) from bambusicolous Shiraia fungi [ 13 ]. Furthermore, some P. fulva bacteria are also relevant to human health and could be an opportunistic human pathogen that may cause infections, such as urosepsis, bacteremia, and adult bacterial meningitis [ 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas9–Mediated Genome Editing for Pseudomonas fulva, a Novel Pseudomonas Species with Clinical, Animal, and Plant–Associated Isolates

Zhang

Muhammad

et al. 2022

IJMS

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As one of the most widespread groups of Gram–negative bacteria, Pseudomonas bacteria are prevalent in almost all natural environments, where they have developed intimate associations with plants and animals. Pseudomonas fulva is a novel species of Pseudomonas with clinical, animal, and plant–associated isolates, closely related to human and animal health, plant growth, and bioremediation. Although genetic manipulations have been proven as powerful tools for understanding bacterial biological and biochemical characteristics and the evolutionary origins, native isolates are often difficult to genetically manipulate, thereby making it a time–consuming and laborious endeavor. Here, by using the CRISPR–Cas system, a versatile gene–editing tool with a two–plasmid strategy was developed for a native P. fulva strain isolated from the model organism silkworm (Bombyx mori) gut. We harmonized and detailed the experimental setup and clarified the optimal conditions for bacteria transformation, competent cell preparation, and higher editing efficiency. Furthermore, we provided some case studies, testing and validating this approach. An antibiotic–related gene, oqxB, was knocked out, resulting in the slow growth of the P. fulva deletion mutant in LB containing chloramphenicol. Fusion constructs with knocked–in gfp exhibited intense fluorescence. Altogether, the successful construction and application of new genetic editing approaches gave us more powerful tools to investigate the functionalities of the novel Pseudomonas species.

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Bacteremia in a human caused by an XDR strain of Pseudomonas fulva

Cited by 8 publications

References 15 publications

Effective Antimicrobial Solutions for Eradicating Multi-Resistant and β-Lactamase-Producing Nosocomial Gram-Negative Pathogens

Effective Antimicrobial Solutions for Eradicating Multi-Resistant and β-Lactamase-Producing Nosocomial Gram-Negative Pathogens

Verona integron encoded metallo Beta lactamase (VIM) and Vietnam extended spectrum Beta lactamase (VEB) producing Pseudomonas balearica from a clinical specimen

CRISPR/Cas9–Mediated Genome Editing for Pseudomonas fulva, a Novel Pseudomonas Species with Clinical, Animal, and Plant–Associated Isolates

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