Bacteria and Their Industrial Importance

Akoijam, Nirmala; Kalita, Debajit; Joshi, S. R.

doi:10.1007/978-981-16-5214-1_2

Cited by 5 publications

(3 citation statements)

References 76 publications

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“…With diverse metabolic pathways and efficient metabolic rates, bacteria can synthesize novel and economically important products, such as enzymes, organic acids, vitamins, antibiotics, antibodies, hormones, carotenoids, steroids, alkaloids, alcoholic beverages, interferons, and vaccines [54]. CRISPR/Cas technology has been proven to be a very robust and effective technique for editing bacterial genomes and optimizing bacterial metabolic pathways.…”

Section: Discussionmentioning

confidence: 99%

Single-Stranded DNA-Binding Proteins Mediate DSB Repair and Effectively Improve CRISPR/Cas9 Genome Editing in Escherichia coli and Pseudomonas

Chai

Zhang

et al. 2023

Microorganisms

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Single-stranded DNA-binding proteins (SSBs) are essential for all living organisms. Whether SSBs can repair DNA double-strand breaks (DSBs) and improve the efficiency of CRISPR/Cas9-mediated genome editing has not been determined. Here, based on a pCas/pTargetF system, we constructed pCas-SSB and pCas-T4L by replacing the λ-Red recombinases with Escherichia coli SSB and phage T4 DNA ligase in pCas, respectively. Inactivation of the E. coli lacZ gene with homologous donor dsDNA increased the gene editing efficiency of pCas-SSB/pTargetF by 21.4% compared to pCas/pTargetF. Inactivation of the E. coli lacZ gene via NHEJ increased the gene editing efficiency of pCas-SSB/pTargetF by 33.2% compared to pCas-T4L/pTargetF. Furthermore, the gene-editing efficiency of pCas-SSB/pTargetF in E. coli (ΔrecA, ΔrecBCD, ΔSSB) with or without donor dsDNA did not differ. Additionally, pCas-SSB/pTargetF with donor dsDNA successfully deleted the wp116 gene in Pseudomonas sp. UW4. These results demonstrate that E. coli SSB repairs DSBs caused by CRISPR/Cas9 and effectively improves CRISPR/Cas9 genome editing in E. coli and Pseudomonas.

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Section: Discussionmentioning

confidence: 99%

Single-Stranded DNA-Binding Proteins Mediate DSB Repair and Effectively Improve CRISPR/Cas9 Genome Editing in Escherichia coli and Pseudomonas

Chai

Zhang

et al. 2023

Microorganisms

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“…Production of single-cell ingredients from microorganisms involves primary cultivation, harvesting and down-stream processing and these steps are largely defined by their taxonomy, biology and physiological requirements of the individual microorganisms themselves and the intended final product(s). [274][275][276] In a broad sense, microalgae and cyanobacteria are either cultivated under phototrophic conditions (e.g. natural or artificial light, pure or waste inorganic carbon and inorganic nutrients), heterotrophic conditions (e.g.…”

Section: Production Of Single-cell Ingredientsmentioning

confidence: 99%

Towards achieving circularity and sustainability in feeds for farmed blue foods

Colombo

Roy

Mráz

et al. 2022

Reviews in Aquaculture

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The aims of this review are to describe the role of 'blue-food production' (animals, plants and algae harvested from freshwater and marine environments) within a circular bioeconomy, discuss how such a framework can help the sustainability and resilience of aquaculture and to summarise key examples of novel nutrient sources that are emerging in the field of fed-aquaculture species. Aquaculture now provides >50% of the global seafood supply, a share that is expected to increase to at least 60% within the next decade.Aquaculture is an important tool for reducing resource consumption in global protein production and increasing resilience to climate change and other global disruptions (i.e. pandemics, geo-political instability). Importantly, blue foods also provide essential nutrition for a growing human population. Blue foods are helping to help the global goal of 'zero hunger' (United Nation's Sustainable Development Goal 2) while reducing the dependency on finite natural resources but further refinement and new solutions are needed to make the industry more 'circular' and sustainable, particularly with respect to sourcing raw materials for aquafeeds. This review describes the feed resources that are available or may be created within a circular bioeconomy framework, their role within the framework and in aquaculture and ultimately, how these resources contribute to de-risking and establishing a resilient aquaculture production chain.

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“…La pandemia por COVID-19, ha tenido como efecto colateral el desarrollo de una mayor conciencia sobre la prevención de enfermedades y la seguridad microbiológica en la salud pública. Respecto a la contaminación microbiológica, las infecciones bacterianas tienen relevancia porque estos microrganismos que se encuentran en todos los ambientes, donde cumplen importantes roles (Akoijam et al, 2022). Sin embargo, algunas bacterias son patógenas y afectan a las poblaciones causando brotes de enfermedades, muertes y pérdidas económicas.…”

Section: Introductionunclassified

Bacterias viables, pero no cultivables: Cuando los patógenos están presentes, pero no son detectados por los análisis convencionales

Gómez¹,

Moleón²,

Viñas³

et al. 2023

LATAM

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Según la OMS, anualmente 600 millones de personas enferman y 420 000 mueren por el consumo de alimentos contaminados; de ellos, 125 000 son niños menores de 5 años. Una fracción de estas cifras, se puede relacionar con la habilidad bacteriana para sobrevivir ante ambientes estresantes, ingresando al estado viable pero no cultivable (VBNC, por sus siglas en inglés), en el que no son detectadas por métodos convencionales. Estos microorganismos afectan a los sectores productivos, provocando pérdidas económicas directas e indirectas, afectando a la salud de las personas expuestas. En este artículo se revisan los esfuerzos por obtener las implicaciones de la existencia de bacterias VBNC en distintas matrices y cómo esta presencia compromete su seguridad microbiológica.

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Bacteria and Their Industrial Importance

Cited by 5 publications

References 76 publications

Single-Stranded DNA-Binding Proteins Mediate DSB Repair and Effectively Improve CRISPR/Cas9 Genome Editing in Escherichia coli and Pseudomonas

Single-Stranded DNA-Binding Proteins Mediate DSB Repair and Effectively Improve CRISPR/Cas9 Genome Editing in Escherichia coli and Pseudomonas

Towards achieving circularity and sustainability in feeds for farmed blue foods

Bacterias viables, pero no cultivables: Cuando los patógenos están presentes, pero no son detectados por los análisis convencionales

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