Bacteria antibiotic resistance: New challenges and opportunities for implant‐associated orthopedic infections

Li, Bingyun; Webster, Thomas J.

doi:10.1002/jor.23656

Cited by 762 publications

(508 citation statements)

References 59 publications

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“…69 Gram-positive bacteria, S. aureus and MRSA, as well as gram-negative bacteria, E. coli and P. aeruginosa, are among the most common pathogens encountered in medical deviceassociated infections. 11,30,[82][83][84][85] (PLL/HA) 50 coatings assembled from all three PLL MWs examined successfully inhibited planktonic bacterial growth for each of these bacteria over 24 h (Fig. 5); these films are highly promising for use as device coatings.…”

Section: Discussionmentioning

confidence: 99%

Influence of poly‐l‐lysine molecular weight on antibacterial efficacy in polymer multilayer films

Alkekhia

Shukla

2019

J Biomedical Materials Res

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Antibacterial coatings can prevent and treat medical device‐associated infections. We examined the antibacterial properties of coatings assembled from poly‐l‐lysine (PLL) and hyaluronic acid (HA). PLL/HA films were fabricated using layer‐by‐layer assembly with three different PLL MWs, differentiated by number of repeat units, that is, 33, 91, and 407 (denoted by PLL30, PLL90, and PLL400). Films assembled with all three PLL MWs completely inhibited the growth of planktonic, gram‐positive Staphylococcus aureus and methicillin‐resistant S. aureus and gram‐negative Pseudomonas aeruginosa and Escherichia coli over a 24‐h exposure. All three film architectures also inhibited S. aureus attachment by ~60–70% compared to non‐film‐coated surfaces, likely attributed to significant film hydration and electrostatic repulsion due to HA. The true differences in antibacterial efficacy between different PLL MWs were observed upon repeated exposure of PLL/HA to S. aureus every 24 h. We found that PLL400 films lost the ability to inhibit planktonic S. aureus growth after one use while PLL30 and PLL90 films were effective over 4–5 and 9–13 repeated exposures, respectively. Our experiments indicated that differences in efficacy were related to low in‐film mobility of PLL400 and also agreed with dissolution timescales for PLL30 and PLL90 films. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1324–1339, 2019.

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Section: Discussionmentioning

confidence: 99%

Influence of poly‐l‐lysine molecular weight on antibacterial efficacy in polymer multilayer films

Alkekhia

Shukla

2019

J Biomedical Materials Res

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“…In recent decades, the presence of multidrug-resistant bacteria has been considered as a major concern in public health (van Duin & Paterson, 2016), reducing the efficacy of antimicrobial treatments (Li & Webster, 2018) and contributing to more than 25,000 deaths in the European Union (EU) each year (Cassini et al., 2019). A wellknown example of bacteria that is resistant to a number of antibiotics is the methicillin-resistant Staphylococcus aureus (MRSA).…”

Section: Introductionmentioning

confidence: 99%

Probabilistic model for the estimation of the consumer exposure to methicillin‐resistant Staphylococcus aureus due to cross‐contamination and recontamination

2019

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The presence of multidrug‐resistant bacteria like methicillin‐resistant Staphylococcus aureus (MRSA) in retail meat is one of the current concerns of the public health authorities. Bacterial cross‐contamination and recontamination during household food preparation could play an important role in the dissemination of such bacteria, and therefore could contribute to a serious health problem, more specifically for immunocompromised people. In order to evaluate the importance of such events, a probabilistic model was developed to estimate the likelihood and extent of cross‐contamination and recontamination and the burden of MRSA from contaminated raw chicken meat via hands and kitchen utensils in a serving (consisting on a slice of bread and a piece of grilled chicken meat) during a household barbecue in Germany. A modular design was used, taking into account the chronological order of the routines during the barbecue event, and Monte Carlo simulations were applied. Available data on the prevalence and burden of MRSA in chicken meat at retail in Germany were used as starting point and were incorporated in the model as probability distributions. The probabilities and extent of bacterial transfer between food items and kitchen utensils (referred to as “Objects”) and the routines performed during food preparation (referred to as “Actions”) specified by their probabilities of occurrence were incorporated as the main input parameters. The model was set up in R 3.5.0 and converted to a standardized format (FSKX file). Therefore, the code can be easily accessed, evaluated, modified, and reused for different purposes. The present study contributes to the quantification of consumer exposure to MRSA through food consumption once contaminated food has entered the household kitchen. Even when the MRSA prevalence and bacterial load in retail chicken meat in Germany are low, resistant bacteria can reach the consumer due to cross‐contamination and recontamination events. The results show that the probability of one CFU to be transferred from the contaminated raw chicken meat to the final serving and the number of MRSA bacteria transferred due to cross‐contamination and recontamination events are in general low, being the contamination of the final serving more likely to occur via bread, rather than via grilled chicken. The results show that the prevalence of MRSA at retail highly influences the probability of the final serving to be contaminated. However, this study also highlights the importance of keeping good hygiene practices during the household food manipulation for reducing the spread of MRSA. The provision of the model in a standardized data format will allow an easy incorporation of the developed model into a complete quantitative microbial risk assessment model that will greatly help to estimate the risk of consumer exposure to MRSA through the consumption of contaminated food.

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“…However, they were higher than those obtained by the same authors on 2018 (Safarpoor Dehkordi et al ) and to those determined by other authors in United States, Poland, India, China and Spain (Hanson et al ; Fijałkowski et al ; Alnakip et al ; Wu et al ; Zehra et al ). This high resistance in food staphylococci samples may be due to both the abuse and incorrect use of disinfectants and antibiotics (Li and Webster ). The rapid dissemination of antibiotic resistance is then supported by the high excretion rate accumulating antibiotics residues in the environment.…”

Section: Resultsmentioning

confidence: 99%

Prevalence and antibiotic resistance profiles ofStaphylococcussp. isolated from food, food contact surfaces and food handlers in a Moroccan hospital kitchen

Touimi

Bennani

Berrada

et al. 2020

Lett Appl Microbiol

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Food poisoning risk related to the consumption of contaminated food with known foodborne pathogens or antibiotic‐resistant bacteria is currently a serious threat for public health. Thus, pathogenic methicillin‐resistant Staphylococcus strains are considered as one of the major cause of foodborne diseases in hospitals. The present study aims to determine the prevalence and the antibiotic resistance patterns of Staphylococcus in various types of hospital food samples, work surfaces and its carriage by food handlers. A total of 608 collected samples including 300 food samples, 238 food contact surfaces and 70 nasal and hand samples were tested. The identified Staphylococcus and their antibiotic resistance patterns were analysed using the agar disk‐diffusion and PCR method was used for mecA resistance gene amplification. The prevalence of S. aureus and the coagulase‐negative staphylococci were 17·33 and 23·33%, respectively. The antibiotic resistance reached 100% towards oxacillin and Penicillin G for both S. aureus and CoNs. The mecA gene was detected in 5·71% (4/70) and 7·69% (4/52) of S. aureus and CoNs strains, respectively. The outcome of this study enlightens isolation of MRSA strains and resistant CoNs from food, food contact surfaces and food handlers. The presence of this resistant species in this critical setting, where products were intended to vulnerable and immunocompromised patients, represents a serious threat to this community. It can be a source of nosocomial infection and more precautions must be taken to prevent staphylococci food contamination mainly in hospitals. Significance and Impact of the Study This is the first study describing the antibiotic resistance patterns of Staphylococcus aureus and coagulase‐negative Staphylococcus isolated from hospital food, food contact surfaces and food handlers samples in a Moroccan hospital kitchen. High levels of multi‐resistance were reported. The alarming outcome of this study emphasizes the crucial need of implementing an approach to fight multidrug‐resistant staphylococci mainly in healthcare settings, where the community have already compromised health issues.

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Bacteria antibiotic resistance: New challenges and opportunities for implant‐associated orthopedic infections

Cited by 762 publications

References 59 publications

Influence of poly‐l‐lysine molecular weight on antibacterial efficacy in polymer multilayer films

Influence of poly‐l‐lysine molecular weight on antibacterial efficacy in polymer multilayer films

Probabilistic model for the estimation of the consumer exposure to methicillin‐resistant Staphylococcus aureus due to cross‐contamination and recontamination

Prevalence and antibiotic resistance profiles ofStaphylococcussp. isolated from food, food contact surfaces and food handlers in a Moroccan hospital kitchen

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