Bacteria associated with ectomycorrhizas of slash pine (Pinus elliottii) in south-eastern Queensland, Australia

Izumi, Hironari; Cairney, John; Killham, Ken; Moore, Edward R. B.; Alexander, Ian J.; Anderson, Ian C.

doi:10.1111/j.1574-6968.2008.01122.x

Cited by 33 publications

(22 citation statements)

References 35 publications

(50 reference statements)

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“…This number is higher than what have been found in previous studies on bacteria associated with EcM, using HTS2345, T-RFLP27, RFLP9 and DGGE analysis9. Several of the bacterial phyla detected in our study are known to be relatively abundant and ubiquitous in soil, such as Acidobacteria, Actinobacteria, Bacteroidetes, Proteobacteria and Verrucomicrobia4647.…”

Section: Discussioncontrasting

confidence: 73%

“…Multiple well-known symbioses harbor rich and diverse bacterial populations. These include lichens127, fungal endophytes8, as well as mycorrhizal fungi910111213.…”

mentioning

confidence: 99%

“…Their association level with the mycorrhizal symbiosis ranges from loosely to tightly, and several bacteria have been reported to be able to promote EcM formation1126. The bacterial diversity within ectomycorrhizae has been explored with various techniques9102327, however, as far as we know, only one study have explored bacteria associated with ectomycorrhizae by means of high throughput sequencing techniques23. Even though this study provided some first insight to the bacterial communities associated with EcM fungi, only a few root tips were studied with a corresponding limited fungal taxonomic coverage, as bacteria of only two EcM fungi were investigated.…”

mentioning

confidence: 99%

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Different bacterial communities in ectomycorrhizae and surrounding soil

Vik

Logares

Blaalid

et al. 2013

Sci Rep

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Several eukaryotic symbioses have shown to host a rich diversity of prokaryotes that interact with their hosts. Here, we study bacterial communities associated with ectomycorrhizal root systems of Bistorta vivipara compared to bacterial communities in bulk soil using pyrosequencing of 16S rRNA amplicons. A high richness of Operational Taxonomic Units (OTUs) was found in plant roots (3,571 OTUs) and surrounding soil (3,476 OTUs). The community composition differed markedly between these two environments. Actinobacteria, Armatimonadetes, Chloroflexi and OTUs unclassified at phylum level were significantly more abundant in plant roots than in soil. A large proportion of the OTUs, especially those in plant roots, presented low similarity to Sanger 16S rRNA reference sequences, suggesting novel bacterial diversity in ectomycorrhizae. Furthermore, the bacterial communities of the plant roots were spatially structured up to a distance of 60 cm, which may be explained by bacteria using fungal hyphae as a transport vector. The analyzed ectomycorrhizae presents a distinct microbiome, which likely influence the functioning of the plant-fungus symbiosis.

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Section: Discussioncontrasting

confidence: 73%

“…Multiple well-known symbioses harbor rich and diverse bacterial populations. These include lichens127, fungal endophytes8, as well as mycorrhizal fungi910111213.…”

mentioning

confidence: 99%

mentioning

confidence: 99%

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Different bacterial communities in ectomycorrhizae and surrounding soil

Vik

Logares

Blaalid

et al. 2013

Sci Rep

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“…Several Pseudomonas and Burkholderia spp. were found to be associated with fungal hyphae, probably growing on fungal exudates (Singh and Arora, 2001;De Boer et al, 2005;Izumi et al, 2008). The fact that the numbers of bacteria belonging to these genera did not increase upon introduction of Absidia hyphae makes it unlikely that the growth response of collimonads was due to the assimilation of exudates.…”

Section: Discussionmentioning

confidence: 91%

Mycophagous growth of Collimonas bacteria in natural soils, impact on fungal biomass turnover and interactions with mycophagous Trichoderma fungi

et al. 2008

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Bacteria of the genus Collimonas are widely distributed in soils, although at low densities. In the laboratory, they were shown to be mycophagous, that is, they are able to grow at the expense of living hyphae. However, so far the importance of mycophagy for growth and survival of collimonads in natural soil habitats is unknown. Using a Collimonas-specific real-time PCR assay, we show here that the invasion of field soils by fungal hyphae (Absidia sp.) resulted in a short-term, significant increase (average fourfold) of indigenous collimonads. No such responses were observed for other soil bacteria studied (Pseudomonas, Burkholderia, PCR-denaturing gradient gel electrophoresis patterns of total bacteria and Burkholderia). Hence, it appears that the stimulation of growth of Collimonas bacteria by fungal hyphae is not common among other soil bacteria. In the same field soils, Trichoderma, a fungal genus known for mycophagous (mycoparasitic) growth, increased upon introduction of Absidia hyphae. Hence, mycophagous growth by Collimonas and Trichoderma can occur in the same soils. However, in controlled experiments (sand microcosms), collimonads appeared to have a negative effect on mycophagous growth of a Trichoderma strain. The effect of mycophagous growth of collimonads on fungal biomass dynamics was studied in sand microcosms using the same Absidia sp. as a test fungus. The growth of collimonads did not cause a significant reduction in the Absidia biomass. Overall, the study indicates that mycophagous nutrition may be important for collimonads in natural soils, but the impact on fungal biomass turnover is likely to be minor.

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“…They revealed that the bacterial communities colonizing the ectomycorrhizal roots differed from those of uncolonized roots and that the ectomycorrhizal species differentially impacted the structure of ectomycorrhizosphere bacterial communities (2,11,12,22,35,37). One mycorrhizal species could be colonized by either very similar or contrastingly different bacterial communities (2,11,13).…”

mentioning

confidence: 96%

Distinct Ectomycorrhizospheres Share Similar Bacterial Communities as Revealed by Pyrosequencing-Based Analysis of 16S rRNA Genes

Uroz

Oger

Morin

et al. 2012

Appl Environ Microbiol

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Analysis of the 16S rRNA gene sequences generated from Xerocomus pruinatus and Scleroderma citrinum ectomycorrhizospheres revealed that similar bacterial communities inhabited the two ectomycorrhizospheres in terms of phyla and genera, with an enrichment of the Burkholderia genus. Compared to the bulk soil habitat, ectomycorrhizospheres hosted significantly more Alpha-, Beta-, and Gammaproteobacteria. E ctomycorrhizal fungi are important actors of nutrient cycling in the forest ecosystems. They enhance the nutrient uptake capacity of plants due to their ability to mobilize carbon from organic matter and access chemical elements with low mobility in the soil, such as nutritive cations, phosphorus, and nitrogen (5,15,32). They also connect tree roots to the surrounding soil and form a very specific ecological environment, the ectomycorrhizosphere (19). Apart from its physical and chemical characteristics, which differ from those of the rhizosphere (26, 28), the ectomycorrhizosphere is characterized by diverse fungal and bacterial communities that inhabit the same environment. Consequently, the functioning of the ectomycorrhizal symbiosis is influenced by each partner of the ectomycorrhizal (ECM) complex (6,8, 9).Cultivation-dependent and -independent studies have demonstrated the structuring effect of the mycorrhizal fungi on the soil bacterial communities. They revealed that the bacterial communities colonizing the ectomycorrhizal roots differed from those of uncolonized roots and that the ectomycorrhizal species differentially impacted the structure of ectomycorrhizosphere bacterial communities (2,11,12,22,35,37). One mycorrhizal species could be colonized by either very similar or contrastingly different bacterial communities (2,11,13). Certain ectomycorrhizal species associated with Betula pubescens, such as Piloderma fallax or Pseudotomentella tristis, were colonized by distinct bacterial communities, whereas the bacterial communities colonizing the ectomycorrhizosphere of Tomentellopsis submollis or Lactarius torminosus were more similar (13), thus suggesting that the taxonomic classification of the host mycorrhizal fungi was not always the main structuring parameter of the bacterial communities. Within this context, a comprehensive description of the ectomycorrhizosphere bacterial communities using a pyrosequencing-based approach would detail the structure and diversity of the bacterial communities coexisting in this specific ecological habitat as well as impart access to the rare taxonomic groups.In a recent study, Uroz et al. (36) used pyrosequencing of 16S rRNA fragments to compare the composition of bacterial communities inhabiting the oak (Quercus petraea) rhizosphere and surrounding bulk soil. In the study presented here, we investigated in the same soil core the composition and structure of bacterial communities inhabiting the ectomycorrhizosphere, which is a specific subhabitat of the rhizosphere. Pyrosequencing tags spanning the V5 to V6 hypervariable regions of the 16S rRNA gene were used to compa...

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Bacteria associated with ectomycorrhizas of slash pine (Pinus elliottii) in south-eastern Queensland, Australia

Cited by 33 publications

References 35 publications

Different bacterial communities in ectomycorrhizae and surrounding soil

Different bacterial communities in ectomycorrhizae and surrounding soil

Mycophagous growth of Collimonas bacteria in natural soils, impact on fungal biomass turnover and interactions with mycophagous Trichoderma fungi

Distinct Ectomycorrhizospheres Share Similar Bacterial Communities as Revealed by Pyrosequencing-Based Analysis of 16S rRNA Genes

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