Bacteria associated with marine macroorganisms as potential source of quorum‐sensing antagonists

Singh, Aparna; Singh, Anil Kumar; Nerurkar, Anuradha S.

doi:10.1002/jobm.202000231

Cited by 14 publications

(11 citation statements)

References 28 publications

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“…D. incerta BGCs included a type 3 polyketide synthase (T3PKS) and a terpene. D. incerta has previously been associated with extracellular enzymatic activities that promote efficient composting ( 31 , 32 ), as well as the production of a lactonase enzyme that degrades homoserine lactone molecules, effectors of quorum sensing expressed by Gram-negative bacteria ( 33 ). A. viridans BGCs included a terpene.…”

Section: Resultsmentioning

confidence: 99%

Harnessing diversity and antagonism within the pig skin microbiota to identify novel mediators of colonization resistance to methicillin-resistant Staphylococcus aureus

Wei

Flowers

Knight

et al. 2023

mSphere

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The microbiota mediate multiple aspects of skin barrier function, including colonization resistance to pathogens such as Staphylococcus aureus . The endogenous skin microbiota limits S. aureus colonization via competition and direct inhibition. Novel mechanisms of colonization resistance are promising therapeutic targets for drug-resistant infections, such as those caused by methicillin-resistant S. aureus (MRSA). Here, we developed and characterized a swine model of topical microbiome perturbation and MRSA colonization. As in other model systems, topical antimicrobial treatment had a little discernable effect on community diversity though the overall microbial load was sensitive to multiple types of intervention, including swabbing. In parallel, we established a porcine skin culture collection and screened 7,700 isolates for MRSA inhibition. Using genomic and phenotypic criteria, we curated three isolates to investigate whether prophylactic colonization would inhibit MRSA colonization in vivo . The three-member consortium together, but not individually, provided protection against MRSA colonization, suggesting cooperation and/or synergy among the strains. Inhibitory isolates were represented across all major phyla of the pig skin microbiota and did not have a strong preference for inhibiting closely related species, suggesting that relatedness is not a condition of antagonism. These findings reveal the porcine skin as an underexplored reservoir of skin commensal species with the potential to prevent MRSA colonization and infection. IMPORTANCE The skin microbiota is protective against pathogens or opportunists such as S. aureus , the most common cause of skin and soft tissue infections. S. aureus can colonize normal skin and nasal passages, and colonization is a risk factor for infection, especially on breach of the skin barrier. Here, we established a pig model to study the competitive mechanisms of the skin microbiota and their role in preventing colonization by MRSA. This drug-resistant strain is also a livestock pathogen, and swine herds can be reservoirs of MRSA carriage. From 7,700 cultured skin isolates, we identified 37 unique species across three phyla that inhibited MRSA. A synthetic community of three inhibitory isolates provided protection together, but not individually, in vivo in a murine model of MRSA colonization. These findings suggest that antagonism is widespread in the pig skin microbiota, and these competitive interactions may be exploited to prevent MRSA colonization.

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Section: Resultsmentioning

confidence: 99%

Harnessing diversity and antagonism within the pig skin microbiota to identify novel mediators of colonization resistance to methicillin-resistant Staphylococcus aureus

Wei

Flowers

Knight

et al. 2023

mSphere

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“…The percentage of QSI active strains from each sponge colony of NP1 and NP6 was 21.8% and 43.5%, respectively. This relatively high percentage of marine bacteria exhibiting QS inhibitory activity is not unexpected as sponge-associated microbes are known to produce quorum sensing quenching compounds ( Saurav et al, 2016 ; Borges and Simões, 2019 ; Ong et al, 2019 ; Singh et al, 2020 ). This relatively high percentage of QSI active bacterial strains was also detected from both the cup and stem regions of the sponge colonies.…”

Section: Discussionmentioning

confidence: 86%

Assessing the Diversity and Biomedical Potential of Microbes Associated With the Neptune’s Cup Sponge, Cliona patera

Katermeran

Deignan

et al. 2021

Front. Microbiol.

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Marine sponges are known to host a complex microbial consortium that is essential to the health and resilience of these benthic invertebrates. These sponge-associated microbes are also an important source of therapeutic agents. The Neptune’s Cup sponge, Cliona patera, once believed to be extinct, was rediscovered off the southern coast of Singapore in 2011. The chance discovery of this sponge presented an opportunity to characterize the prokaryotic community of C. patera. Sponge tissue samples were collected from the inner cup, outer cup and stem of C. patera for 16S rRNA amplicon sequencing. C. patera hosted 5,222 distinct OTUs, spanning 26 bacterial phyla, and 74 bacterial classes. The bacterial phylum Proteobacteria, particularly classes Gammaproteobacteria and Alphaproteobacteria, dominated the sponge microbiome. Interestingly, the prokaryotic community structure differed significantly between the cup and stem of C. patera, suggesting that within C. patera there are distinct microenvironments. Moreover, the cup of C. patera had lower diversity and evenness as compared to the stem. Quorum sensing inhibitory (QSI) activities of selected sponge-associated marine bacteria were evaluated and their organic extracts profiled using the MS-based molecular networking platform. Of the 110 distinct marine bacterial strains isolated from sponge samples using culture-dependent methods, about 30% showed quorum sensing inhibitory activity. Preliminary identification of selected QSI active bacterial strains revealed that they belong mostly to classes Alphaproteobacteria and Bacilli. Annotation of the MS/MS molecular networkings of these QSI active organic extracts revealed diverse classes of natural products, including aromatic polyketides, siderophores, pyrrolidine derivatives, indole alkaloids, diketopiperazines, and pyrone derivatives. Moreover, potential novel compounds were detected in several strains as revealed by unique molecular families present in the molecular networks. Further research is required to determine the temporal stability of the microbiome of the host sponge, as well as mining of associated bacteria for novel QS inhibitors.

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“…How microbes interfere with quorum signals is an area of intense research [ 94 ], and is well documented among marine bacteria [ 33 , 95 , 96 ]. Different mechanisms are employed by these bacteria to do this, which can be divided into (1) interfering with QS circuitry by producing inhibitory molecules, thus inhibiting the ability of a bacterium to sense or synthesize QS signals, and (2) producing enzymes which degrade QS signals (often called quorum quenching).…”

Section: Can Microalgae Modulate Quorum-sensing?mentioning

confidence: 99%

How Do Quorum-Sensing Signals Mediate Algae–Bacteria Interactions?

Dow

2021

Microorganisms

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Quorum sensing (QS) describes a process by which bacteria can sense the local cell density of their own species, thus enabling them to coordinate gene expression and physiological processes on a community-wide scale. Small molecules called autoinducers or QS signals, which act as intraspecies signals, mediate quorum sensing. As our knowledge of QS has progressed, so too has our understanding of the structural diversity of QS signals, along with the diversity of bacteria conducting QS and the range of ecosystems in which QS takes place. It is now also clear that QS signals are more than just intraspecies signals. QS signals mediate interactions between species of prokaryotes, and between prokaryotes and eukaryotes. In recent years, our understanding of QS signals as mediators of algae–bacteria interactions has advanced such that we are beginning to develop a mechanistic understanding of their effects. This review will summarize the recent efforts to understand how different classes of QS signals contribute to the interactions between planktonic microalgae and bacteria in our oceans, primarily N-acyl-homoserine lactones, their degradation products of tetramic acids, and 2-alkyl-4-quinolones. In particular, this review will discuss the ways in which QS signals alter microalgae growth and metabolism, namely as direct effectors of photosynthesis, regulators of the cell cycle, and as modulators of other algicidal mechanisms. Furthermore, the contribution of QS signals to nutrient acquisition is discussed, and finally, how microalgae can modulate these small molecules to dampen their effects.

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Bacteria associated with marine macroorganisms as potential source of quorum‐sensing antagonists

Cited by 14 publications

References 28 publications

Harnessing diversity and antagonism within the pig skin microbiota to identify novel mediators of colonization resistance to methicillin-resistant Staphylococcus aureus

Harnessing diversity and antagonism within the pig skin microbiota to identify novel mediators of colonization resistance to methicillin-resistant Staphylococcus aureus

Assessing the Diversity and Biomedical Potential of Microbes Associated With the Neptune’s Cup Sponge, Cliona patera

How Do Quorum-Sensing Signals Mediate Algae–Bacteria Interactions?

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