Bacteria associated with oak and ash on a TCE-contaminated site: characterization of isolates with potential to avoid evapotranspiration of TCE

Abstract

The characterization of the isolates obtained in this study shows that the bacterial community associated with Oak and Ash on a TCE-contaminated site, was strongly enriched with TCE-tolerant strains. However, this was not sufficient to degrade all TCE before it reaches the leaves. A possible strategy to overcome this evapotranspiration to the atmosphere is to enrich the plant-associated TCE-degrading bacteria by in situ inoculation with endophytic strains capable of degrading TCE.

Endophytic and rhizoplane bacteria associated with Cytisus striatus growing on hexachlorocyclohexane-contaminated soil: isolation and characterisation

“…The fact that densities of culturable root endophytes were significantly greater than either stem or leaf endophytes supports the theory that these bacteria are thought to colonise plants primarily through the root system via natural and artificial wound sites, root hairs and at epidermal junctions (Pan et al 1997;Porteous-Moore et al 2006;Weyens et al 2009a). Members of some genera were common to both root compartments, such as Amycolaptosis, Bacillus, Burkholderia, Dyella, Leucobacter and Rhizobium.…”

“…Trace metal resistance of the strains was tested using 284 agar medium supplemented with 1 mM Cd, Co, Ni or Zn (added as CdSO 4 .8/3H 2 O, CoCl 2 .6H 2 O, NiSO 4 .6H 2 O and ZnSO 4 .7H 2 O) and incubated at 28°C for 7 days. The number of strains is given in each bar those published for other species (herbaceous plants and woody tree species) (Kuklinsky-Sobral et al 2004;Sharma et al 2005;Sun et al 2010;Weyens et al 2009a). Germida et al (1998) found higher densities of culturable bacteria in the rhizoplane compared to the root interior of canola or wheat.…”

Phytoremediation: recent advances in plant-endophytic synergistic interactions

“…2) Weyens et al 2009c). Plants host a variety of microorganisms due to their nutrient-rich environment and allow a broad and complex spectrum of interactions to exist between themselves and their associated Brader et al (2014) and Weyens et al (2009c) microorganisms, especially bacteria (Weyens et al 2009a). Bacteria not only reside on the roots but many of them enter the plant to seek nutrients and dwell inside.…”

Plant-Microbe Partnerships for Enhanced Biodegradation of Polychlorinated Biphenyls

“…Various physicochemical processes including soil washing, electrokinetic methods, chemical reduction or oxidation of contaminant, incinerations, etc., appear to be expensive, environment invasive, and many times nonspecifi c, producing secondary contaminants (Pandey et al 2009 ;Weyens et al 2009Weyens et al , 2010a. Thus, there is a constant thrust to develop alternative strategies for remediating contaminated sites.…”

“…Phytoremediation is the use of plants to remediate polluted soils which is an eco-friendly and cost-effective technology and currently receiving considerable global attention (Glick 2010 ;Yousaf et al 2011 ;Khan et al 2013 ). It is an in situ solar-powered remediation technology, requiring minimal site disturbance and maintenance resulting in high public acceptance (Weyens et al 2009(Weyens et al , 2010a. Since conventional remediation options currently available are frequently expensive and environmentally invasive, phytoremediation emerges out to be a valuable alternative, especially for the treatment of large contaminated areas with diffused pollution (Weyens et al 2010a , b ;Nesterenko-Malkovskaya et al 2012 ).…”