Bacteria Associated with Selected Rivers in Akure, Nigeria and their Alkysulphatase Activity/Production

Assessment of Surfactant Degrading Potential of Fungal Isolates from Detergent Contaminated Soil in Ondo State, Nigeria

“…Fungi act as potential degraders by excreting enzymes from vesicles on top of their hyphae to their environment to break down pollutants to simpler forms; which are being absorbed for growth and biomass accumulation [12]. Variations in the alkylsulphatase enzyme production capacities of the various fungal isolates could be as a result of their genetic makeup [11]. There were variations in the quantity of alkylsulphatase enzyme activities produced by the fungal isolates and this could be as a result of molecular mass of alkylsulfatase; which is found to vary in different bacterial species and genera [13].…”

“…Some certain quantity (Fifty millilitres) of the broth culture was collected at the end of specific time intervals (twelve hours) and it was centrifuged for 15 minutes at 4°C. The supernatant was decanted off [11]. Cell pellets were collected with one millilitre (1 ml) of tris buffer and it was later subjected to homogenisation for a period of 15 minutes.…”

“…Cell pellets were collected with one millilitre (1 ml) of tris buffer and it was later subjected to homogenisation for a period of 15 minutes. The homogenised pellets were collected and centrifuged for 15 minutes at 4 o C. The supernatant was collected and kept for the enzyme assay [11].…”

“…The chloroform layer formed was collected into a tube by carefully releasing the separating funnel tap and the absorbance which indicates the quantity of enzyme produced was read at 600 nm. Enzyme activity was determined by evaluating the rate of SDS (sodium dodecyl sulphate) elimination with respect to quantity of enzyme used and time of incubation [11].…”