Bacteria belonging to the genus Burkholderia are obligatory symbionts of the eriococcids Acanthococcus aceris Signoret, 1875 and Gossyparia spuria (Modeer, 1778) (Insecta, Hemiptera, Coccoidea)

Michalik, Katarzyna; Szklarzewicz, Teresa; Kalandyk-Kołodziejczyk, Małgorzata; Jankowska, Władysława; Michalik, Anna

doi:10.1016/j.asd.2016.04.002

Cited by 25 publications

(54 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…With the exception of one lineage of aphids (Gibson & Hunter, ; Hongoh & Ishikawa, ; Vogel & Moran, ), each family in the large suborder Sternorrhyncha relies on a bacterial primary symbiont(s) to supplement its dietary needs, including closely related families within the diverse superfamily Coccoidea (Baumann, ; Dhami, Turner, Deines, Beggs, & Taylor, ; Douglas, ; Michalik, Szklarzewicz, Kalandyk‐Kołodziejczyk, Jankowska, & Michalik, ; Moran et al., ; Rosas‐Pérez, Rosenblueth, Rincón‐Rosales, Mora, & Martínez‐Romero, ). Hence, Coccidae is the only family so far in the Sternorrhyncha (or at least the two major subfamilies studied here: Ceroplastinae and Coccinae) which seems to be dominated by fungal rather than bacterial symbionts.…”

Section: Discussionmentioning

confidence: 99%

An exceptional family: Ophiocordyceps‐allied fungus dominates the microbiome of soft scale insects (Hemiptera: Sternorrhyncha: Coccidae)

et al. 2017

View full text Add to dashboard Cite

Hemipteran insects of the suborder Sternorrhyncha are plant sap feeders, where each family is obligately associated with a specific bacterial endosymbiont that produces essential nutrients lacking in the sap. Coccidae (soft scale insects) is the only major sternorrhynchan family in which obligate symbiont(s) have not been identified. We studied the microbiota in seven species from this family from Israel, Spain and Cyprus, by high-throughput sequencing of ribosomal genes, and found that no specific bacterium was prevalent and abundant in all the tested species. In contrast, an Ophiocordyceps-allied fungus sp.-a lineage widely known as entomopathogenic-was highly prevalent. All individuals of all the tested species carried this fungus. Phylogenetic analyses showed that the Ophiocordyceps-allied fungus from the coccids is closely related to fungi described from other hemipterans, and they appear to be monophyletic, although the phylogenies of the Ophiocordyceps-allied fungi and their hosts do not appear to be congruent. Microscopic observations show that the fungal cells are lemon-shaped, are distributed throughout the host's body and are present in the eggs, suggesting vertical transmission. Taken together, the results suggest that the Ophiocordyceps-allied fungus may be a primary symbiont of Coccidae-a major evolutionary shift from bacteria to fungi in the Sternorrhyncha, and an important example of fungal evolutionary lifestyle switch.

show abstract

Section: Discussionmentioning

confidence: 99%

An exceptional family: Ophiocordyceps‐allied fungus dominates the microbiome of soft scale insects (Hemiptera: Sternorrhyncha: Coccidae)

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Until now, studies have only focused on gut microbial communities in bees (Engel and Moran, ), although in other insects bacterial communities also reside beyond the gut in specific cells and tissues within the host and influence host physiology. The presence of bacterial species has been reported in the hemolymph of aquatic invertebrates (Lokmer et al, ; Lokmer and Wegner, ; Wang and Wang, ), the hemolymph and reproductive organs of red palm weevil (Montagna et al, ), the salivary glands and ovaries of malaria mosquito (Tchioffo et al, ) and the fat body cells of scale insects (Michalik et al, ). Above‐characterized bacterial communities differ according to changing internal and external factors, but their definite functionalities in relation to their tissue of occurrence and host remain unclear.…”

Section: Introductionmentioning

confidence: 99%

A prokaryotic–eukaryotic relation in the fat body of Bombus terrestris

Parmentier

Billiet

Smagghe

et al. 2018

Environ Microbiol Rep

View full text Add to dashboard Cite

The interaction between the insect host and its microbiota plays a central role in insect health and is mostly studied in relation to the digestive system. Nonetheless, there are numerous microorganisms occupying multiple habitats in and on insects. We studied microbial communities in the gut and fat body of bumblebees (Bombus terrestris) using the V4 region of the 16S rRNA gene on the Illumina MiSeq platform. In one of the two study locations, the fat body microbial composition was marked by the dominant presence of Arsenophonus sp. and Phyllobacterium sp. Bumblebees infected with Apicystis bombi, a eukaryotic parasite multiplying in the fat body, had a significant higher relative abundance of Arsenophonus sp. compared with the non-infected individuals. In general, the infection of A. bombi correlated with a more interlinked microbial association network, as we observed an increase of significant associations between the relative abundance of bacteria present in the gut and fat body of infected bumblebees. The causality within this potential prokaryotic-eukaryotic relation is important when assessing the health impact on bees.

show abstract

“…Thus, the occurrence of specialized bacteriocytes may be treated as important evidence that G. brachypodii acquired both of the symbionts much earlier than the hemipterans mentioned above. It is worth noting that in other members of the Eriococcidae family, such as Acanthococcus aceris and Gossyparia spuria , the symbiotic bacteria Burkholderia do not occur in specialized bacteriocytes, but are dispersed in the fat body of these insects (Michalik et al ., ). This situation may indicate that, in contrast to other scale insects, the symbiosis between Gossyparia and Acanthococcus and Burkholderia symbionts is relatively recent.…”

Section: Discussionmentioning

confidence: 97%

“…Both symbionts of G. brachypodii , like other obligate symbionts of insects, are transovarially transmitted between generations. The mode of symbiont transmission in G. brachypodii is very similar to that of Burkholderia symbionts in the previously described representatives of the Eriococcidae family – A. aceris and G. spuria (Michalik et al ., ). In all of the species mentioned above, bacteria invade the ovarioles in their neck region.…”

Section: Discussionmentioning

confidence: 97%

Coexistence of novel gammaproteobacterial and Arsenophonus symbionts in the scale insect Greenisca brachypodii (Hemiptera, Coccomorpha: Eriococcidae)

Michalik

Schulz

Michalik

et al. 2018

Environmental Microbiology

Self Cite

View full text Add to dashboard Cite

Scale insects are commonly associated with obligate, intracellular microorganisms which play important roles in complementing their hosts with essential nutrients. Here we characterized the symbiotic system of Greenisca brachypodii, a member of the family Eriococcidae. Histological and ultrastructural analyses have indicated that G. brachypodii is stably associated with coccoid and rod-shaped bacteria. Phylogenetic analyses have revealed that the coccoid bacteria represent a sister group to the secondary symbiont of the mealybug Melanococcus albizziae, whereas the rod-shaped symbionts are close relatives of Arsenophonus symbionts in insects - to our knowledge, this is the first report of the presence of Arsenophonus bacterium in scale insects. As a comparison of 16S and 23S rRNA genes sequences of the G. brachypodii coccoid symbiont with other gammaprotebacterial sequences showed only low similarity (∼90%), we propose the name 'Candidatus Kotejella greeniscae' for its tentative classification. Both symbionts are transovarially transmitted from one generation to the next. The infection takes place in the neck region of the ovariole. The bacteria migrate between follicular cells, as well as through the cytoplasm of those cells to the perivitelline space, where they form a characteristic 'symbiont ball'. Our findings provide evidence for a polyphyletic origin of symbionts of Eriococcidae.

show abstract

Bacteria belonging to the genus Burkholderia are obligatory symbionts of the eriococcids Acanthococcus aceris Signoret, 1875 and Gossyparia spuria (Modeer, 1778) (Insecta, Hemiptera, Coccoidea)

Cited by 25 publications

References 54 publications

An exceptional family: Ophiocordyceps‐allied fungus dominates the microbiome of soft scale insects (Hemiptera: Sternorrhyncha: Coccidae)

An exceptional family: Ophiocordyceps‐allied fungus dominates the microbiome of soft scale insects (Hemiptera: Sternorrhyncha: Coccidae)

A prokaryotic–eukaryotic relation in the fat body of Bombus terrestris

Coexistence of novel gammaproteobacterial and Arsenophonus symbionts in the scale insect Greenisca brachypodii (Hemiptera, Coccomorpha: Eriococcidae)

Contact Info

Product

Resources

About