Bacteria in cancer therapy: beyond immunostimulation

Torres, Wheeler; Lameda, Victor; Olivar, Luis Carlos; Navarro, Carla; Fuenmayor, Jorge; Pérez, Adrián; Mindiola, Andres; Rojas, Milagros; Martínez, María Sofía; Velasco, Manuel; Rojas, Joselyn; Bermúdez, Valmore

doi:10.20517/2394-4722.2017.49

Cited by 29 publications

(29 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…cytotoxic agents, prodrug-converting enzymes, etc. (Torres et al, 2018; Zhou et al, 2018; Kramer et al, 2018). Such treatment modalities could be incorporated by extending the model, to provide a more accurate description of the therapeutic action.…”

Section: Discussionmentioning

confidence: 99%

“…This mechanism arises as a pure physical effect from the competition for space and nutrients between cancer and bacteria cells and could be optimized to obtain the highest tumor volume reduction. Currently, even though researchers are aware of the benefits coming from active bacteria migration towards hypoxic regions in tumors (Forbes, 2010; Kramer et al, 2018), this knowledge has not been efficiently exploited in the clinical trials carried out so far (Torres et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…by realising toxins and therapeutic agents, or stimulating an immune response). (Forbes, 2010; Osswald et al, 2015; Torres et al, 2018; Zhou et al, 2018).…”

Section: Mathematical Modelmentioning

confidence: 99%

“…However, such treatments also caused some fatalities and the limited understanding of the mechanisms of action of these therapies shifted research efforts towards other strategies - especially radiotherapy (Kramer et al, 2018). In the last few years the use of live bacteria for cancer treatment has gained new interest, and several bacterial strains have been tested in animal models and even advanced to clinical trials (Torres et al, 2018). Nevertheless, clinical development of such therapies is still facing significant issues due to infection-associated toxicities and incomplete knowledge of infection dynamics (Kramer et al, 2018; Zhou et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Investigating the physical effects in bacterial therapies for avascular tumors

Mascheroni

Meyer‐Hermann

Hatzikirou

2019

Preprint

View full text Add to dashboard Cite

Tumor-targeting bacteria elicit anticancer effects by infiltrating hypoxic regions, releasing toxic agents and inducing immune responses. As the mechanisms of action of bacterial therapies are still to be completely elucidated, mathematical modeling could aid the understanding of the dynamical interactions between tumor cells and bacteria in different cancers. Here we propose a mathematical model for the anti-tumor activity of bacteria in tumor spheroids. We consider constant infusion and time-dependent administration of bacteria in the culture medium, and analyze the effects of bacterial chemotaxis and killing rate. We show that active bacterial migration towards tumor hypoxic regions is necessary for successful spheroid infiltration and that intermediate chemotaxis coefficients provide the smallest spheroid radii at the end of the treatment. We report on the impact of the killing rate on final spheroid composition, and highlight the emergence of spheroid size oscillations due to competing interactions between bacteria and tumor cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…by realising toxins and therapeutic agents, or stimulating an immune response). (Forbes, 2010; Osswald et al, 2015; Torres et al, 2018; Zhou et al, 2018).…”

Section: Mathematical Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Investigating the physical effects in bacterial therapies for avascular tumors

Mascheroni

Meyer‐Hermann

Hatzikirou

2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The immune system plays a critical role in tumor immunosurveillance. Abnormal cells are recognized and eliminated with the help of innate and adaptive immunity in a continuous and bidirectional manner [ 93 ]. Tumor evolution occurs in three phases of “immunoediting” namely—elimination, equilibrium, and escape phase.…”

Section: Mechanism Of Tumor Suppressionmentioning

confidence: 99%

Microbes as Medicines: Harnessing the Power of Bacteria in Advancing Cancer Treatment

Sawant

Patil

Gupta

et al. 2020

IJMS

View full text Add to dashboard Cite

Conventional anti-cancer therapy involves the use of chemical chemotherapeutics and radiation and are often non-specific in action. The development of drug resistance and the inability of the drug to penetrate the tumor cells has been a major pitfall in current treatment. This has led to the investigation of alternative anti-tumor therapeutics possessing greater specificity and efficacy. There is a significant interest in exploring the use of microbes as potential anti-cancer medicines. The inherent tropism of the bacteria for hypoxic tumor environment and its ability to be genetically engineered as a vector for gene and drug therapy has led to the development of bacteria as a potential weapon against cancer. In this review, we will introduce bacterial anti-cancer therapy with an emphasis on the various mechanisms involved in tumor targeting and tumor suppression. The bacteriotherapy approaches in conjunction with the conventional cancer therapy can be effective in designing novel cancer therapies. We focus on the current progress achieved in bacterial cancer therapies that show potential in advancing existing cancer treatment options and help attain positive clinical outcomes with minimal systemic side-effects.

show abstract

Phenotypic characterization of auxotrophic mutant of nontyphoidal Salmonella and determination of its cytotoxicity, tumor inhibiting cytokine gene expression in cell line models

et al. 2021

View full text Add to dashboard Cite

An auxotrophic mutant of non-typhoidal Salmonella (NTS) strain was phenotypically characterized in this study. The characterization was based on phenotype, morphology, motility, bio lm forming ability, growth kinetics, etc. The phenotypic results from the above experiments determined that the mutant showed variation in phenotypic characters from that of wild-type strain. Subsequently, mutant and wild-type NTS were subjected to epithelial cell invasion and intracellular replication assays. The real time PCR analysis was also performed to analyse expression of tumor inhibiting cytokine genes and virulence genes post bacterial infection in cell lines. The mutant showed highest invasion potential than wild-type NTS whereas the replication of mutant was slower in both the cell lines. Similar to the wild-type strain, the mutant also retained the cytotoxic potential when analysed in vitro. Furthermore, the expression of proin ammatory cytokine genes such as TNF-α and IL-1β was upsurged with the downregulation of anti-in ammatory cytokine genes like TGF-β, IL-6 and IL-10 post infection of the mutant strain in cell lines. In addition, virulence genes of Salmonella pathogenicity island one and two of mutant were downregulated in vitro except invA in HeLa cell line. Therefore, the auxotrophic mutant showed positive attributes of a potential antitumor agent in terms of expressing tumor inhibiting cytokine genes when assessed in vitro. Though the study did not check the tumor inhibitory effect of NTS strain directly, ndings of the study emphasizes on the development of a novel strain of NTS with less virulence and more immunogenic traits to inhibit tumor cells.

show abstract

Bacteria in cancer therapy: beyond immunostimulation

Cited by 29 publications

References 0 publications

Investigating the physical effects in bacterial therapies for avascular tumors

Investigating the physical effects in bacterial therapies for avascular tumors

Microbes as Medicines: Harnessing the Power of Bacteria in Advancing Cancer Treatment

Phenotypic characterization of auxotrophic mutant of nontyphoidal Salmonella and determination of its cytotoxicity, tumor inhibiting cytokine gene expression in cell line models

Contact Info

Product

Resources

About