Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation

Masngut, Nasratun; Manap, Sulastri Abdul; Man, Rohaida Che; Shaarani, Shalyda Md

doi:10.17485/ijst/2017/v10i7/111224

Cited by 9 publications

(8 citation statements)

References 13 publications

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“…Isolate C3 was selected as xylanase producer via submerged fermentation. In the previous study, Isolate C3 has shown the highest xylanase activity among other isolates from landfill soil after qualitative and quantitative analysis for xylanase production [8]. Isolate C3 is a gram-negative bacteria and has bacilli shape observed under a microscope.…”

Section: Bacteria Isolates From Landfill Soilmentioning

confidence: 86%

“…Landfill isolate, known as Isolate C3 was obtained from our collection in Bioprocessing Laboratory, Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang, Malaysia. This isolate was locally isolated from landfill soil as described in Masngut et al [8] and maintained on nutrient agar (Merks, Germany) slants at 4 °C. Isolate C3 was selected as xylanase producer via submerged fermentation.…”

Section: Bacteria Isolates From Landfill Soilmentioning

confidence: 99%

“…It was prepared and sterilized in each cotton plugged 250 mL Erlenmeyer flask. The fermentation precedes at initial pH of 7.0, 37 °C and an agitation rate of 150 rpm as recommended by Masngut et al [8]. The OFAT running strategy was divided into six experimental runs as summarized in Table 1.…”

Section: Experimental Design Of Screening Of Culture Conditions For Xmentioning

confidence: 99%

“…Although many microorganisms have been isolated and exploited for enzyme production, there is still a need for novel strains capable in producing enhanced levels of the enzyme [7]. The bacteria used in this work was previously isolated from landfill soil [8] in Malaysia which is the natural occurring physical covering of the earth's surface and are home to a diversity of microorganisms [9]. The landfill is a site for disposal of solid waste that comprises a high composition of xylan [8] and the high possibility of the xylanase to degrade them.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The bacteria used in this work was previously isolated from landfill soil [8] in Malaysia which is the natural occurring physical covering of the earth's surface and are home to a diversity of microorganisms [9]. The landfill is a site for disposal of solid waste that comprises a high composition of xylan [8] and the high possibility of the xylanase to degrade them. Thus, it would be the right place to discover new strain for xylanase producer from the landfill area.…”

Section: ■ Introductionmentioning

confidence: 99%

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Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

2019

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Culture conditions including initial pH media, incubation period, inoculum size, type of carbon source, type of nitrogen source and its concentration, which affect xylanase production were screened via the one-factor-at-a-time approach. The bacteria used in the production of xylanase was isolated from the landfill site at Sg. Ikan, Kuala Terengganu, Malaysia. Three characterizations of the landfill soil were investigated for their moisture content, ash content, and pH. The culture conditions range used in the experimental work were between 6–30 h for the incubation period, with initial pH between 5–9, inoculum size between 1–20% v/v, carbon, nitrogen sources, and nitrogen source concentration between 1–5% w/v. Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the release of xylose under standard assay conditions. The landfill soil was observed to have pH between pH 3.4–7.2 with a moisture content between 12.4–33.7% and ash ranged between 3.5–4.3%. Results showed that the highest xylanase activity within studied ranges was recorded at 25.91±0.0641 U/mL with 10% (v/v) inoculum size, 1% (w/v) xylose as sole carbon source, mixture of 1% (w/v) peptone and 0.25% (w/v) ammonium sulphate as nitrogen sources, which was carried out at initial pH of 8.0 for 24 h incubation.

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Section: Bacteria Isolates From Landfill Soilmentioning

confidence: 86%

Section: Bacteria Isolates From Landfill Soilmentioning

confidence: 99%

Section: Experimental Design Of Screening Of Culture Conditions For Xmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

2019

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Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil

Rosli

Man

Masngut

2020

IOP Conf. Ser.: Mater. Sci. Eng.

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Two-level full factorial design was applied to screen the important parameters for production of xylanase by newly isolated Bacillus sp. from landfill soil. Five production parameters were considered: initial pH media (pH 5–9), inoculum size (5%–10% v/v), incubation period (18–30 h), temperature (30-50 °C) and agitation speed (0-200 rpm). Xylanase activity was estimated using dinitrosalicylic acid (DNS) based on the xylose released under specified assay conditions. Based on the factorial analysis, it was observed that the significant parameters in the xylanase production were temperature, agitation speed and initial pH of media. Meanwhile, the interaction between temperature and initial pH of media gave the highest influenced to the xylanase production. The model revealed that the highest xylanase activity can be achieved at 123.34 U/mL with initial pH media of 7.0, 30 h incubation period, 5% (v/v) inoculum size, agitation speed of 100 rpm at 40 °C. Confirmation run produced the highest experimental xylanase activity by Bacillus sp. at 123.10 U/mL with 0.17% of error than the predicted one. Hence, the model was reliably predicting the xylanase production.

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Effects of Enzyme Loading, Incubation Time and Incubation Temperature on Sawdust Hydrolysis by Locally Produced Bacterial Xylanase

Shaarani

Min

Man

et al. 2021

JCEIB

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The present study aims to investigate the enzymatic hydrolysis conditions of bacterial xylanase on alternative cheaper substrate which is hardwood sawdust (SD) in order to produce reducing sugars (xylose). The bacterial xylanase was produced and secreted from the Bacillus sp. The wood industry in Malaysia has become a major source of foreign exchange across the globe for developing the countries. Therefore, more wood residues (sawdust) are produced during the logging and processing of wood. Most of the sawdust will be disposed into the landfills. In actual, the sawdust can be utilised into more valuable products such as in producing reducing sugars. Thus, previous researches have studied on xylose production from wooden sawdust using commercial xylanases, but only few with the bacterial xylanase. Therefore, a study on the best conditions of enzymatic hydrolysis in producing xylose from sawdust using bacterial xylanase is essential. Prior to the enzymatic hydrolysis, the hardwood sawdust was pre-treated by autoclave at 121°C for 20 min in order to breakdown the lignin linkage and obtain the hemicellulosic xylan (delignification). The enzymatic hydrolysis conditions such as enzyme loading, incubation time and incubation temperature were experimented by One-Factor-At-Time (OFAT) method. Based on the experiment, the fifth cycle pre-treated autoclaved sawdust showed 5.5-fold higher than the untreated sawdust. The best enzymatic hydrolysis conditions for xylose production were enzyme loading of 1.4%, incubation time of 30 min, and incubation temperature of 56.9 °C. These conditions also succeeded in producing 2.5-fold higher xylose than the one without the enzymatic hydrolysis.

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Bacteria Isolation from Landfill for Production of Industrial Enzymes for Waste Degradation

Cited by 9 publications

References 13 publications

Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

Screening of Culture Conditions for Production of Xylanase from Landfill Soil Bacteria

Factorial experimental design for xylanase production by Bacillus sp. isolated from Malaysia landfill soil

Effects of Enzyme Loading, Incubation Time and Incubation Temperature on Sawdust Hydrolysis by Locally Produced Bacterial Xylanase

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