Bacteria of an anaerobic 1,2-dichloropropane-dechlorinating mixed culture are phylogenetically related to those of other anaerobic dechlorinating consortia.

Abstract

A 16S-rDNA-based molecular study was performed to determine the bacterial diversity of an anaerobic, 1,2-dichloropropane-dechlorinating bioreactor consortium derived from sediment of the River Saale, Germany. Total community DNA was extracted and bacterial 16S rRNA genes were subsequently amplified using conserved primers. A clone library was constructed and analysed by sequencing the 16S rDNA inserts of randomly chosen clones followed by dot blot hybridization with labelled polynucleotide probes. The phylogenetic analysis revealed significant sequence similarities of several as yet uncultured bacterial species in the bioreactor to those found in other reductively dechlorinating freshwater consortia. In contrast, no close relationship was obtained with as yet uncultured bacteria found in reductively dechlorinating consortia derived from marine habitats. One rDNA clone showed S97 % sequence similarity to Dehalobacter species, known for reductive dechlorination of tri-and tetrachloroethene. These results suggest that reductive dechlorination in microbial freshwater habitats depends upon a specific bacterial community structure.

Biotransformation of Polychlorinated Dioxins and Microbial Community Dynamics in Sediment Microcosms at Different Contamination Levels

“…Interestingly, the majority of the clones detected in this study were most similar to those previously found in chlorinated solvent-contaminated aquifer 16) and in microbial communities capable of reductive dechlorination of PCDD/Fs 60) , TCDD 6) , PCBs 29,34) , chlorobenzenes 4,53) and 1,2-dichloropropane 48) . In view of the results of these previous studies and our observations, anaerobic or semi-anaerobic environments polluted with organohalorides may share a number of common phylogenetic and physiologic groups of bacteria irrespective of the kinds of chlorinated pollutants.…”

Bacterial Diversity and Sulfur Cycling in a Mesophilic Sulfide-Rich Spring

“…These clones belonged to the following divisions: Cytophaga, Spirochaetes, Planctomyces, Verrucomicrobiae, Firmicutes, Actinomyces, and candidate division TM7. At the source, the concentrations of methane, ethane, and propane were high and many of the source clones were closely related to clones from hydrocarbon-rich sites or hydrocarbon-degrading enrichments (15,53,61,62,70,77). The presence of hydrocarbons could therefore have an impact on shaping this microbial community.…”

Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge

“…The Chloroflexi of core group a (OTUs III and VI) seem to be more characteristic of wastewater sludge because their closest relatives were found in activated sludge and in an anaerobic digester treating municipal sludge. Nevertheless, it should be noted that all the Chloroflexi sequences are affiliated with the order Anaerolineales and more precisely with the groups Eub 4 and SHA-24 that were detected in anaerobic consortia which degrade toluene and 1,2 dichloropropane (Ficker et al, 1999;Schlotelburg et al, 2000).…”