Bacteria of Human Physiological Microflora Liberate Immunomodulating Peptides

Pulverer, G.; Beuth, Josef; Roszkowski, W.; Burrichter, H.; Roszkowski, K.; Yassin, Atteyet-Alla Fetouh; Ko, H.L.; Jeljaszewicz, J.

doi:10.1016/s0934-8840(11)80048-4

Cited by 14 publications

(10 citation statements)

References 13 publications

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“…The suppressive effect was non-dialyzable, indicating that immunomodulating peptides (mol. wt < 6500 Da), as described for bacteria of the human physiological microflora [45], are not involved. The suppression did not seem to be due to cytotoxicity of H. pylori antigens per se, since cell viability and cytokine release by monocytes were not affected by the bacterium at the concentrations employed.…”

Section: Discussionmentioning

confidence: 98%

Immune suppressive effects of Helicobacter pylori on human peripheral blood mononuclear cells

Knipp

Birkholz

Kaup

et al. 1993

Med Microbiol Immunol

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Helicobacter pylori, the causative agent of type-B gastritis and duodenal ulcer in man is described as a bacterium able to stimulate the human immune system. This study demonstrates that H. pylori besides this property possesses an immune suppressive activity. The in vitro proliferation of human peripheral blood mononuclear cells to purified protein derivative of tuberculin (PPD), phytohemagglutinin, and concanavalin A was reduced in a dose-dependent manner by bacteria which had been inactivated by incubation at 56 degrees C as well as by a soluble cytoplasmic fraction of H. pylori. The immune suppressive effect on the mitogen-induced proliferation could be increased by preincubation of the mononuclear cells with H. pylori. The observed effect does not seem to be a specific phenomenon depending on prior exposure of the blood donors to H. pylori, since suppression occurred with mononuclear cells of H. pylori-infected patients as well as of antibody-negative healthy control individuals. The suppressive activity was non-dialyzable, heat-labile (100 degrees C, 30 min) and sensitive to trypsin. Furthermore, the treatment at 100 degrees C caused an increase in the capability of H. pylori to induce lymphoproliferation. This fact indicates that the suppressive factor is also effective on H. pylori antigens. While exogenous interleukin-2, could to a certain extent, restore the responsiveness of the lymphocytes after PPD-stimulation in the presence of H. pylori, the addition of interleukin-1 had no effect on the suppressed lymphoproliferation. Cell-separation and cell-mixing experiments indicated that an influence on monocytes rather than on T cells is the major cause of the observed suppressive effect. Although the immunological mechanisms involved in H. pylori-associated gastritis are not clearly defined, it is reasonable to presume that suppression of host defense mechanisms may contribute to the pathogenesis of this disease.

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Section: Discussionmentioning

confidence: 98%

Immune suppressive effects of Helicobacter pylori on human peripheral blood mononuclear cells

Knipp

Birkholz

Kaup

et al. 1993

Med Microbiol Immunol

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“…Platí to také o transmastných kyselinách a nasycených tucích. Tyto substance suprimují aktivitu makrofágů, baktericidní a cytotoxické funkce lymfoidních buněk a podporují Th 2 odpověď oproti Th 1 , jak prokázali už před mnoha lety K. Roszkowský (38) a G. Pulverer (39). Všechny alterace imunitního systému vyvolané složkami takto ošet-řených potravin připravují terén pro vznik chronických chorob jako je diabetes 2. typu, reumatoidní artritida a některá nádorová onemocnění (40).…”

Section: Přehledové Práceunclassified

Health Aspects of Thermal Food Processing

Turek¹,

Šíma²,

Bencko³

2014

Hygiena

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SOUHRNSoučasné poznatky moderně chápané nutriční imunologie mají zásadní význam z hlediska aktivit v oblasti ochrany a podpory zdraví. Existuje nespočet dokladů, že neadekvátní nebo snížený nutriční příjem se neodráží jen klasickými příznaky proteinové a energetické malnutrice, ale také nepříznivým ovlivněním až selháváním imunitních funkcí.Stále rostoucí počet in vitro a in vivo experimentálních a klinických studií dokládá, že produkty neenzymatických reakcí, které vznikají při technologickém a kulinárním zpracování potravin mohou hrát významnou roli při vzniku a globálním šíření chronických nesdělných onemocnění. Prokazuje se, že hlavní patogenetický význam mají tzv. konečné produkty pokročilé glykace (AGE, Advanced Glycation End products), lipoxidace (ALE, Advanced Lipoxidation End products), a oxidace proteinů (AOPP, Advanced Oxidation Protein Products), které představují nejdůležitější nutriční složky kumulující se ve vnitřním prostředí organismu v průběhu jeho celého života. V současnosti jsou považovány za nejčastější složky poškozující zdraví a zároveň představující jednu z význam-ných příčinu růstu incidence chronických nesdělných nemocí ve všech zemích, které přijaly "západní" životní styl.Klíčová slova: chronická neinfekční onemocnění -etiologie, technologizovaná strava, Advanced Glycation End products (AGE), Advanced Lipoxidation End products (ALE), Advanced Oxidation Protein Products (AOPP) SUMMARYThe recent results of research in the field of nutritional immunology have a wide impact on health protection and promotion-related activities. There is a great deal of evidence showing that the inadequate or lowered nutritional intake is reflected in both protein-energetic malnutrition and immune system disorders.A growing number of in vitro/in vivo experimental and clinical studies documents that the products of non-enzymatic reactions formed during technological and culinary processing of food may play a major role in the emergence and global spread of chronic non-communicable diseases.Evidence mainly points to the pathogenic role of advanced glycation end products (AGE), advanced lipoxidation end products (ALE), and advanced oxidation protein products (AOPP), which represent the most important dietary compounds that accumulate within the internal milieu of an organism over a whole life span. Currently, these factors are regarded as one of a row of causations with strong negative influence on health and the most frequent causative agents of increasing chronic disease burden in all countries which adopt a 'westernized lifestyle'.

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“…In the course of studying the underlying (patho )physiological mechanism we found that certain species of the indigenous GI tract microflora provide low molecular weight substances which apparently trigger basic immune responses (1,23). Accordingly, the hypothesis was discussed that eradication of the GI tract microflora might suppresses local and/or systemic immune responses since the generation of immunotriggering microbial substances ceased.…”

Section: Antibiotics and Immune Systemmentioning

confidence: 99%

Microflora-associated Defense Stimulating Factors

Pulverer¹,

Ko²,

Beuth³

1997

Scandinavian Journal of Gastroenterology

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Mucosal surfaces are habitats for the physiological microflora and are closely related to the mucosal immune compartment (mucosa-associated lymphoid tissue, MALT). Recently, considerable evidence has accumulated showing that various members of the physiological microflora liberate low molecular weight substances which, apparently, are essential for the adequate immune response of the host. Antibiotic decontamination (e.g. of the BALB/c mouse gastrointestinal tract) results in a lack of generation of immunopriming microbial substances leading to immunosuppression. Biochemical analysis of the microbial substances revealed reproducible chromatographic fractions which selectively influence maturation, proliferation and activation of mononuclear immune cells.

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Bacteria of Human Physiological Microflora Liberate Immunomodulating Peptides

Cited by 14 publications

References 13 publications

Immune suppressive effects of Helicobacter pylori on human peripheral blood mononuclear cells

Immune suppressive effects of Helicobacter pylori on human peripheral blood mononuclear cells

Health Aspects of Thermal Food Processing

Microflora-associated Defense Stimulating Factors

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