Bacteria Recovered from Cultured Gilt-Head Seabream (Sparus aurata) and their Antimicrobial Susceptibilities

Abstract

Objective: Gilt-head seabream (Sparus aurata) is an important species for Turkish aquaculture and bacterial diseases are one of the limiting factors for the production of this species. The aim of this study is the identification of the bacterial disease agents in cultured gilt-head seabream in Turkey and the determination of their antibacterial susceptibilities. Materials and Methods:In this study, 27 diseased gilt-head seabream samples between 3-130 g, showing various clinical symptoms, were examined bacteriologically. Bacterial isolates recovered from moribund fish samples were identified by using standard bacteriological methods and API 20E rapid identification strips. Antibiotic susceptibility tests of 10 different substances against these isolates were determined by using Kirby-Bauer disc diffusion method.Results: Vibrio anguillarum, V. scophthalmi, V. logei, V. harveyi, Pseudomonas anguilliseptica, P. stutzeri, Staphylococcus sp. and Micrococcus luteus isolates were isolated from diseased fish samples as pure and mixed infection. It was detected that API 20E may give erroneous results for fish pathogenic bacteria since they are not included in the database of the kit. Generally, enrofloxacin, florphenicol and oxytetracycline were found to be the most effective antibiotics against bacterial isolates. Conclusion:This study is the first report for V. scophthalmi, V. logei, V. harveyi, P. stutzeri and M. luteus infections of gilt-head seabream in Turkey.

Isolation and Characterization of Micrococcus luteus from Oreochromis niloticus in Egypt

“…According to results of this study, M. luteus isolates were sensitive to penicillin, amoxicillin/clavulanic acid, ampicillin/sulbactam, norfloxacin, chloramphenicol and tetracycline while they were resistant to cefotaxime, amikacin, tobramycin, erythromycin and ciprofloxacin. Our results agreed with findings of Çanak and Akayli (2018) who found that M. luteus isolates were sensitive to ampicillin and tetracycline while resistant to ciprofloxacin and also with findings of Akayli et al (2020) who found that M. luteus was sensitive to chloramphenicol and tetracycline while resistant to ciprofloxacin while in contrast to our results, Aydin et al (2005) found that M. luteus was sensitive to cefotaxime, tobramycin and erythromycin while resistant to oxytetracycline and chloramphenicol. These different results may be attributed to the different usage of these antibiotics in aquaculture in these areas and to difference of M. luteus strains.…”

“…This difference may be attributed to the differences in locality, sampling season, water quality, water temperature and/or stocking density. In this study, the infected O. niloticus exhibited clinical signs and PM lesions similar to those reported byÇanak and Akayli (2018),Pękalaa et al (2018),Akayli et al (2019) andAkayli et al (2020) in M. luteus infections in rainbow trout, common dentex, gilthead seabream and sharpsnout seabream respectively.…”

A novel bacterial infection in cultured Nile tilapia, Oreochromis niloticus in New Valley, Egypt

“…Internally, there were congested and sometimes enlarged liver, spleen, and kidney in addition to distended gall bladder. Most of these clinical signs and post-mortem findings were observed in M. luteus infections in rainbow trout (Pękala et al, 2018), common dentex (Akayli et al, 2019), gilthead seabream (Çanak and Akayli 2018) and sharpsnout seabream (Akayli et al, 2020) but skin depigmentation, congestion and erosion of the fins, corneal opacity and gall bladder distention weren't observed in these studies and this may be attributed to the differences in fish species and environmental conditions which determine infection severity.…”

“…Infection with M. luteus causes excessive skin pigmentation, exophthalmia, gill damage, abdomen distention, pale, elongated spleen, and kidney (Austin and Austin, 2007). M. luteus infection was reported in some countries in rainbow trout, Oncorhynchus mykiss, (Aydin et al, 2005;Mousavi et al, 2010;Türk et al, 2013 andPekala et al, 2018), common dentex, Dentex dentex (Akayli et al, 2019), gilthead seabream, Sparus aurata (Çanak and Akayli, 2018), O. niloticus (Parra-Laca et al, 2020and Wanja et al, 2020 and in sharpsnout seabream Diplodus puntazzo Yardimci, 2018 andAkayli et al, 2020). But, for our knowledge there were no studies on M. luteus infections in fishes in Egypt.…”

Effect of Dietary Canarium indicum L. on the Growth, Health, and Resistance of Asian Seabass Challenged with Vibrio alginolyticus

“…The treatments challenged with V. alginolyticus showed clinical signs such as anorexia, lesions operculum, dropsy abdomen, dropsy internal organ (Figure 2), and death. These clinical signs revealed that vibriosis in fish is characterized by behavioral changes such as anorexia (Ransangan and Mustafa, 2009), lesions operculum (Turgay and Karataş, 2016), dropsy abdomen (Canak and Akayli, 2018), and dropsy internal organ (Silvaraj et al, 2020).…”