Bacteria repellent layer made of flagellin

Kovács, Boglárka; Patko, Daniel; Klein, Ágnes; Kakasi, Balázs; Saftics, András; Kurunczi, Sándor; Vonderviszt, Ferenc; Horváth, Róbert

doi:10.1016/j.snb.2017.11.027

Cited by 11 publications

(11 citation statements)

References 37 publications

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“…PCP-LIP refers to a thin, quasi-planar polycarboxylate polymer (PCP) coating on the waveguide where lipophilic (LIP) groups are pre-immobilized to the PCP chains. This type of sensor chip can be used for capturing ligands through hydrophobic interactions (according to our previous results, flagellins can be efficiently captured in this way 52,62,63 ). The PCH chip carries a thick polycarboxylate-based hydrogel coating with free carboxylic functions to which proteins can be covalently coupled through EDC/NHS (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride/N-hydroxysuccinimide) linking chemistry.…”

Section: Methodsmentioning

confidence: 98%

Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers

Jankovics

Kovács

Saftics

et al. 2020

Sci Rep

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Reliable measurement of the binding kinetics of low molecular weight analytes to their targets is still a challenging task. Often, the introduction of labels is simply impossible in such measurements, and the application of label-free methods is the only reliable choice. By measuring the binding kinetics of Ni(II) ions to genetically modified flagellin layers, we demonstrate that: (1) Grating-Coupled Interferometry (GCI) is well suited to resolve the binding of ions, even at very low protein immobilization levels; (2) it supplies high quality kinetic data from which the number and strength of available binding sites can be determined, and (3) the rate constants of the binding events can also be obtained with high accuracy. Experiments were performed using a flagellin variant incorporating the C-terminal domain of the nickel-responsive transcription factor NikR. GCI results were compared to affinity data from titration calorimetry. We found that besides the low-affinity binding sites characterized by a micromolar dissociation constant (Kd), tetrameric FliC-NikRC molecules possess high-affinity binding sites with Kd values in the nanomolar range. GCI enabled us to obtain real-time kinetic data for the specific binding of an analyte with molar mass as low as 59 Da, even at signals lower than 1 pg/mm2.

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Section: Methodsmentioning

confidence: 98%

Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers

Jankovics

Kovács

Saftics

et al. 2020

Sci Rep

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“…Another approach for anti-adhesion coatings is based on the hydrophobicity where the superhydrophobic or hydrophilic coatings aggravate the attachment for bacteria to the surface. A more recent study uses a repellent film that is based on flagellin [87]. In this study, a novel type of bacteria repellent layer that was made of the flagellin is examined.…”

Section: Anti-adhesion/ Bacteria Repelling Coatingsmentioning

confidence: 99%

A Brief Recap of Microbial Adhesion and Biofilms

2019

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Food and beverage industries operate their production units under stringent hygiene standards to verify high-quality products. However, the presence of biofilms can cause hygienic problems in the industries in the case of pathogenic organisms. Microorganisms can form biofilms, which are resistant to cleaning and disinfection. Microorganisms in biofilms are closely packed in a matrix that acts as a barrier to cleaning and disinfection. Biofilms are observed in processing equipment and open surfaces, resulting in food safety problems or weakening of production efficiency. This review provides a recap of the biofouling process, including the production mechanisms and control techniques of microbial adhesion. Microbial adhesion and colonization are the sine qua non of the establishment of bacterial pathogenesis and this report focuses on their prevention.

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“…Recent studies demonstrated that a compact and oriented flagellin protein layer -mimicking the surface of bacterial flagellum -has excellent cell repellent capabilities [34], [35], [36], [37]. By genetically engineering the molecule, peptid sequences (for example RGD motif) can be incorporated into this cell repellent layer facilitating receptor specific cellular adhesion.…”

Section: Fig1 Basic Processes Of Cell Adhesion: the Artificially Tail...mentioning

confidence: 99%

A practical review on the measurement tools for cellular adhesion force

Ungai-Salánki

Péter

Gerecsei

et al. 2019

Advances in Colloid and Interface Science

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Cell-cell and cell-matrix adhesions are fundamental in all multicellular organisms. They play a key role in cellular growth, differentiation, pattern formation and migration. Cell-cell adhesion is substantial in the immune response, pathogen-host interactions, and tumor development. The success of tissue engineering and stem cell implantations strongly depends on the fine control of live cell adhesion on the surface of natural or biomimetic scaffolds. Therefore, the quantitative and precise measurement of the adhesion strength of living cells is critical, not only in basic research but in modern technologies, too. Several techniques have been developed or are under development to quantify cell adhesion. All of them have their pros and cons, which has to be carefully considered before the experiments and interpretation of the recorded data. Current review provides a guide to choose the appropriate technique to answer a specific biological question or to complete a biomedical test by measuring cell adhesion.

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Bacteria repellent layer made of flagellin

Cited by 11 publications

References 37 publications

Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers

Grating-coupled interferometry reveals binding kinetics and affinities of Ni ions to genetically engineered protein layers

A Brief Recap of Microbial Adhesion and Biofilms

A practical review on the measurement tools for cellular adhesion force

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