Bacterial adherence to bioinert and bioactive materials studied in vitro

Oga, Masayoshi; Arizono, Takeshi; Sugioka, Yôichi

doi:10.3109/17453679308993623

Cited by 37 publications

(22 citation statements)

References 14 publications

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“…For example, Oga et al [9,10] used a rinse method with saline, whereas Olson et al [11] used vortexing for 1 minute without sonication. Moreover, sonication with or without vortexing has been used widely [14,16].…”

Section: Discussionmentioning

confidence: 99%

Improved Detection of Biofilm-formative Bacteria by Vortexing and Sonication: A Pilot Study

Kobayashi

Oethinger

Tuohy

et al. 2009

Clinical Orthopaedics &Amp; Related Research

110

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Bacteria such as staphylococci commonly encountered in orthopaedic infections form biofilms and adhere to bone implants and cements. Various methods to disrupt the biofilm and enhance bacterial detection have been reported. We will describe the effectiveness of vortexing and sonication to improve the detection of biofilmformative bacteria from polymethylmethacrylate by conventional quantitative bacterial culture and real-time quantitative PCR. We used a single biofilm-formative Staphylococcus aureus strain and 20 polymethylmethacrylate coupons as an in vitro biofilm model; four coupons were used for each of two control groups or three experimental sonication times (1, 5, and 30 minutes). Vortexing the cement without sonication increased the yield of adherent bacteria to a considerable extent. The combination of vortexing and sonication further enhanced the yield regardless of the duration of sonication. Quantitative conventional cultures correlated with quantitative PCR assay. The combination of vortexing and sonication to disrupt the bacterial biofilm followed by quantitative PCR and/or culture seems to be a sensitive method for detecting bacteria adherent to bone cement.

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Section: Discussionmentioning

confidence: 99%

Improved Detection of Biofilm-formative Bacteria by Vortexing and Sonication: A Pilot Study

Kobayashi

Oethinger

Tuohy

et al. 2009

Clinical Orthopaedics &Amp; Related Research

110

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“…A recent survey of the literature suggests that i.he contribution of implant-adsorbed host proteins to modulating staphylococcal attachment to orthopaedic devices is still underestimated (1,6,10,24,30). At ithe time of insertion, orthopaedic implants are exposed to numerous plasma and blood cell components, which lead to the coating of the artificial surfaces with a conditioning layer (30).…”

Section: Discussionmentioning

confidence: 99%

“…The association of metallic implants with bacterial infections may be influenced by interfacial reactions with host defense mechanisms (7,ll) and by specifically elaborated microbial factors promoting bacterial colonization (6,10,24). Bacterial adhesion is the first step leading to colonization on and infection around orthopaedic materials (4,lO).…”

mentioning

confidence: 99%

Novel animal model for studying the molecular mechanisms of bacterial adhesion to bone‐implanted metallic devices: Role of fibronectin in Staphylococcus aureus adhesion

Fischer

Vaudaux

Magnin

et al. 1996

Journal Orthopaedic Research

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Infection around metallic implants is a rare but severe complication of orthopaedic surgery. A novel animal model mimicking conditions of internal fixation devices was developed to evaluate the role of host proteins adsorbed on metallic devices in promoting adhesion and colonization of the material surfaces by Staphylococcus aureus. Small plates made of pure titanium were either fixed (three screws per plate) onto the iliac bones of guinea pigs or implanted into their subcutaneous space as controls. Five to 6 weeks after surgery, the plates and screws were removed from the previously killed animals, carefully rinsed in buffer, and tested in an in vitro assay of S. aureus adhesion to metallic surfaces. To evaluate the role of fibronectin in staphylococcal adhesion to explanted plates and screws, a mutant of S. aureus that is specifically defictive in fibronectin adhesion due to decreased expression of the fibronectin adhesin was compared with its isogenic parental strain. A significant reduction in adhesion of the fibronectin adhesin-defective mutant compared with the parental strain occurred on both the subcutaneously implanted and bone-implanted metallic plates. The results of this specific biological assay suggest that fibronectin is present on bone-implanted metallic devices and promotes attachment of S. aureus to their surfaces. This novel experimental model should help, to characterize several parameters of bacterial adhesion to metallic orthopaedic devices and to develop novel anti-adhesive strategies for preventing such infections.

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“…of biofilm, which was evaluated using a previously described spectrophotometric method [7]. As materials used in orthopaedic surgery have different affinities for bacteria [18], we assessed discs with different compositions and surface roughnesses similar to those found in commonly used prostheses.…”

Section: Methodsmentioning

confidence: 99%

Does Dithiothreitol Improve Bacterial Detection from Infected Prostheses? A Pilot Study

Drago

Romanò

Mattina

et al. 2012

Clinical Orthopaedics &Amp; Related Research

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Bacterial adherence to bioinert and bioactive materials studied in vitro

Cited by 37 publications

References 14 publications

Improved Detection of Biofilm-formative Bacteria by Vortexing and Sonication: A Pilot Study

Improved Detection of Biofilm-formative Bacteria by Vortexing and Sonication: A Pilot Study

Novel animal model for studying the molecular mechanisms of bacterial adhesion to bone‐implanted metallic devices: Role of fibronectin in Staphylococcus aureus adhesion

Does Dithiothreitol Improve Bacterial Detection from Infected Prostheses? A Pilot Study

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