Bacterial adherence to hydrocarbons: a useful technique for studying cell surface hydrophobicity

Rosenberg, Mel

doi:10.1016/0378-1097(84)90026-0

Cited by 132 publications

(170 citation statements)

References 18 publications

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“…The BATH assay, developed by Rosenberg et al (18), gives a cell hydrophobicity index (A% = percentage of adhesion). Cell suspensions were made based on a 0.5 McFarland standard, and 4 mL of cell suspension was transferred to individual test tubes, which contained 1 mL of octane.…”

Section: Hydrophobicity Assessmentmentioning

confidence: 99%

Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation

Ghasemian

Naghoni

Rahvar

et al. 2015

Jundishapur J Microbiol

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Background: Biofilm formation is a major virulence factor in different bacteria. Biofilms allow bacteria to resist treatment with antibacterial agents. The biofilm formation on glass and steel surfaces, which are extremely useful surfaces in food industries and medical devices, has always had an important role in the distribution and transmission of infectious diseases. Objectives: In this study, the effect of coating glass and steel surfaces by copper nanoparticles (CuNPs) in inhibiting the biofilm formation by Listeria monocytogenes and Pseudomonas aeruginosa was examined. Materials and Methods:The minimal inhibitory concentrations (MICs) of synthesized CuNPs were measured against L. monocytogenes and P. aeruginosa by using the broth-dilution method. The cell-surface hydrophobicity of the selected bacteria was assessed using the bacterial adhesion to hydrocarbon (BATH) method. Also, the effect of the CuNP-coated surfaces on the biofilm formation of the selected bacteria was calculated via the surface assay. Results: The MICs for the CuNPs according to the broth-dilution method were ≤ 16 mg/L for L. monocytogenes and ≤ 32 mg/L for P. aeruginosa. The hydrophobicity of P. aeruginosa and L. monocytogenes was calculated as 74% and 67%, respectively. The results for the surface assay showed a significant decrease in bacterial attachment and colonization on the CuNP-covered surfaces. Conclusions: Our data demonstrated that the CuNPs inhibited bacterial growth and that the CuNP-coated surfaces decreased the microbial count and the microbial biofilm formation. Such CuNP-coated surfaces can be used in medical devices and food industries, although further studies in order to measure their level of toxicity would be necessary.

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Section: Hydrophobicity Assessmentmentioning

confidence: 99%

Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation

Ghasemian

Naghoni

Rahvar

et al. 2015

Jundishapur J Microbiol

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“…Bacteria were resuspended in phosphate-urea-magnesium buffer (pH 7-2) as described by Rosenberg et al (1980) and Rosenberg (1984): 1.2 ml of a suspension of E470 0.3 was placed in a round-bottomed glass test tube (10 mm diameter) and various volumes of octane or hexadecane were added; after incubation for 10 min at room temperature, the tubes were mixed by vigorous vortexing for 120 s (Whirlimixer, Fisons, Loughborough, Leics). After allowing 20 min for the separation of aqueous and hydrocarbon phases, the aqueous phase was carefully removed and its E470 recorded.…”

Section: Hy Dropho Hicity Studiesmentioning

confidence: 99%

The influence of the O and K antigens of Klebsiella aerogenes on surface hydrophobicity and susceptibility to phagocytosis and antimicrobial agents

Williams

Lambert

Haigh

et al. 1986

Journal of Medical Microbiology

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“…For these reasons attention has tended to be directed towards the role of more hydrophobic organisms in dental plaque ecology (Gibbons & Etherden, 1983;Rosenberg et al, 1983;Rosenberg, 1984). However, secretion by hydrophilic strains of much higher concentrations of a number of biologically active components could have particular significance.…”

Section: K W Knox L N Hardy and A J Wicken Discussionmentioning

confidence: 99%

“…Particular attention was given to the protein component designated PI, which is indistinguishable (Forester et al, 1983) from the previously described antigens 1/11 (Russell, M. W. et al, 1980) and B (Russell, R. R. B., 1979) and whose production was markedly affected by the growth conditions (Hardy et al, 1981 ;Forester et al, 1983). The total amount of extracellular protein of molecular mass 260 kDa produced by the different strains was also estimated and compared with the hydrophobicity of the organisms as measured by adhesion to hexadecane (Rosenberg, 1984). There is evidence that surface proteins contribute to hydrophobicity (Weerkamp et al, 1982;Wadstrom et al 1984), and the decrease in hydrophobicity of two strains of S. mutans serotype c on repeated subculturing has been shown to be accompanied by the release of greater amounts of several proteins including antigens B (McBride et al, 1984(McBride et al, , 1985, A and C, and also lipoteichoic acid (LTA) (Russell, R. R. B.…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies on the Protein Profiles and Hydrophobicity of Strains of Streptococcus mutans Serotype c

Knox¹,

Hardy²,

Wicken³

1986

Microbiology

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Twelve strains of Streptococcus mutans serotype c were grown in batch culture with glucose at constant pH (6.0) and a number of properties compared. On the basis of their cellular and extracellular protein profiles, the strains were divided into three groups, I, I1 and 111, containing five, four and three strains, respectively. The extracellular protein profiles for a particular strain differed if the organisms were grown either at pH 6.0 with fructose instead of glucose or with glucose but without pH control. The total amount of extracellular protein produced by group I11 strains grown in glucose-containing medium at pH 6.0 was several times that produced by strains of groups I and 11, which were also more hydrophobic. One of the potentially important proteins is P1, also called antigen B or 1/11, and it was shown to be entirely in the culture fluid of group I11 strains but mostly cell-associated from strains of groups I and 11. Approximately half of the cell-associated fraction of P1 could be removed with hot sodium dodecyl sulphate.

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Bacterial adherence to hydrocarbons: a useful technique for studying cell surface hydrophobicity

Cited by 132 publications

References 18 publications

Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation

Evaluating the Effect of Copper Nanoparticles in Inhibiting Pseudomonas aeruginosa and Listeria monocytogenes Biofilm Formation

The influence of the O and K antigens of Klebsiella aerogenes on surface hydrophobicity and susceptibility to phagocytosis and antimicrobial agents

Comparative Studies on the Protein Profiles and Hydrophobicity of Strains of Streptococcus mutans Serotype c

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