Bacterial Adhesion and Biofilm Formation of Enterococcus faecalis on Zwitterionic Methylmethacrylat and Polysulfones

Abstract

Biofilm-associated implant infections represent a major challenge for healthcare systems around the world due to high patient burden and enormous costs incurred. Enterococcus faecalis (E. faecalis) is the most prevalent enterococcal species identified in biofilm-associated infections. The steadily growing areas of application of implants demand a solution for the control of bacterial infections. Therefore, the development of modified anti-microbial implant materials and the testing of the behavior of different relevant bacterial strains towards them display an indispensable task. Recently, we demonstrated an anti-microbial effect of zwitterionic modified silicone rubber (LSR) against Staphylococcus aureus. The aim of this study was to evaluate bacterial colonization and biofilm formation of another clinically relevant strain, E. faecalis, on this material in comparison to two of the most commonly used thermoplastic polyurethanes (TPUs) and other modified LSR surfaces. By generating growth curves, crystal violet, and fluorescence staining, as well as analyzing the expression of biofilm-associated genes, we demonstrated no anti-microbial activity of the investigated materials against E. faecalis. These results point to the fact that anti-microbial effects of novel implant materials do not always apply across the board to all bacterial strains.

Drug release control and anti-inflammatory effect of biodegradable polymer surface modified by gas phase chemical functional reaction

“…Recently, studies on the biocompatibility, resistance to bacterial infection or physical stress [36], material composition for biological functions [37], surface roughness [38], modulus of elasticity [39], and surface topology/morphology [40] have been conducted to improve the function of implantable materials. In addition to these mechanical and physical functions, drug-holding and delivery capabilities are considered important parameters for predicting biological effects.…”

Understanding the diagnosis of catheter-related bloodstream infection: real-time monitoring of biofilm growth dynamics using time-lapse optical microscopy

“…We consider it is needed to use different growth media for each specie in particular to create the best conditions and nutrients. If we would have used the same media for all microorganisms, they would have shown different growth kinetics, and therefore, this would have mean a potential bias in the study (Woitschach et al, 2022).…”

Study on PTFE Superhydrophobic Coating Modified by IC@dMSNs and its Enhanced Antibacterial Effect

“…), with a relatively small number of fungi (including Candida albicans, Candida smooth, etc.) (22)(23)(24)(25).…”

“…But as bacteria begin to secrete some extracellular matrix, which is rich in mucins and glycoproteins, it strengthens bacterial colonization on the surface of the material, thereby promoting continuous bacterial reproduction and forming colonies. When colonies adhere together through the extracellular matrix, they form bio lms (23,57). Mature bio lms continuously release bacteria, leading to bloodstream infections.…”