Bacterial adhesion on hydrophilic heparinized catheters, with compared with adhesion on silicone catheters, in patients with malignant obstructive jaundice

Abstract

To study the inhibitory effects on bacterial adhesion of a newly devised, hydrophilic heparinized catheter to be used in patients with malignant obstructive jaundice, a randomized controlled study of indwelling endoprostheses was performed, using implantable port-connected heparinized catheters (n = 25) and silicone catheters (n = 21). Catheters withdrawn from patients were cultured for bacteria and examined by electron microscopy for the presence of adherent organisms. In vitro examination of the two type of catheters exposed to suspensions of Eschericia coli and Staphylococcus aureus was performed using electron microscopy and a luminometer. The formation of a biofilm coated with glycocalyces was found in silicone catheters, but not in the heparinized catheters. In vitro experiments demonstrated little bacterial adhesion to the heparinized surface, but significant formation of biofilm on the silicone surface. Anionically charged heparinized catheters have inhibitory effects on bacterial adhesion, and the surface charge of the catheter may be a factor in inhibiting this adhesion.

Clinical significance of bacterial cultures from 28 autologous islet cell transplant solutions

“…Frequently, an ERCP is performed with placement of endoprosthesis to stent localized strictures and facilitates pancreatic drainage. These catheters commonly become obstructed due to sludge adhesion with subsequent aerobic and anaerobic bacterial overgrowth [21,22] . Several studies have examined the microbial fl ora of biliary stents after removal and it has been shown that bacteria can be isolated from the majority of these endoprosthesis [23,24] .…”

Key Role of Teichoic Acid Net Charge in Staphylococcus aureus Colonization of Artificial Surfaces

“…Accordingly, several studies have demonstrated that the use of negatively charged materials, such as ionized plastics, Teflon, or heparinized surfaces, is of particular benefit in reducing colonization (2,11,12,15). Moreover, since the teichoic acid content and the degree of D-alanylation vary among S. aureus strains (16), increased amounts of D-alanine esters may contribute to the capacity of staphylococci to colonize indwelling devices.…”

A novel arterial infusion chemotherapy for the treatment of patients with advanced pancreatic carcinoma after vascular supply distribution via superselective embolization

“…31,32 However, we produced a biliary endoprosthesis in which an H-PSD tube was connected with the implanted port, 16 which enabled us to easily resolve jaundice and thus appeared to contribute to the increase of the survival rate. 31,32 However, we produced a biliary endoprosthesis in which an H-PSD tube was connected with the implanted port, 16 which enabled us to easily resolve jaundice and thus appeared to contribute to the increase of the survival rate.…”