Bacterial Adhesion to Surfactant-modified Silicone Surfaces

Levy, Michael L.; Luu, Truc; Meltzer, Hal S.; Bennett, Rebecca; Bruce, Derek A.

doi:10.1227/01.neu.0000103673.13196.7f

Cited by 12 publications

(9 citation statements)

References 5 publications

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“…Nonetheless there was no significant difference between the two concentrations of P188 at 16 hours (1% vs 3%, p= 0.49). Similar results were reported earlier by [36]. The study investigated the adhesion of Staphylococcus epidermidis on silicone sheets soaked in varying concentrations of Poloxamer 188 solution.…”

Section: Effect Of Poloxamer 188 On Bacterial Adhesion To Clssupporting

confidence: 79%

“…The study investigated the adhesion of Staphylococcus epidermidis on silicone sheets soaked in varying concentrations of Poloxamer 188 solution. At 20% P188, the bacterial adhesion decreased to 3.02% from 22.2% in the control sheets [36]. The reduction in bacterial adhesion in the presence of P188 can be attributed to the ability of PPO groups to be…”

Section: Effect Of Poloxamer 188 On Bacterial Adhesion To Clsmentioning

confidence: 93%

“…adsorbed onto the surface of the silicone-based contact lens allowing the two PEO groups to project outwards into the polymer matrix [25]. The hydrophilic PEO provides a 'steric hindrance', thus preventing the hydrophobic surface of P. aeruginosa from attaching to the surface of the lens [7,36].…”

Section: Effect Of Poloxamer 188 On Bacterial Adhesion To Clsmentioning

confidence: 99%

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Adherence of Pseudomonas aeruginosa onto surfactant-laden contact lenses

Mosuela

Mustafa

Gould

et al. 2018

Colloids and Surfaces B: Biointerfaces

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There is an immense research interest to utilise contact lens (CLs) as a popular platform for ocular drug delivery. However, CLs are the major predisposing factors of bacterial keratitis which is commonly caused by adhesion of microbes such as Pseudomonas aeruginosa and Staphylococcus epidermidis. The aim of the current study is to explore the effect of surfactants; Poloxamer 188, Polysorbate 80 and Tetronic 90R4 (at 0.25% - 3% v/v) on the characteristics of CLs and on the adhesion abilities of Pseudomonas aeruginosa to the lenses' surfaces. CLs were formulated using a hydrophilic monomer; 2-hydroxyethyl methacrylate (HEMA) together with silicone-based polymer such as Poly dimethyl siloxane (PDMS) or 3,3,3-trifluoropropylsilane (FSA) then lenses were polymerized under UV light. The formulated CLs with surfactants were found to have an increased equilibrium water content (EWC) due to hydrophilic moiety present in surfactants. A relationship was deduced between EWC and surface contact angle of lenses containing surfactants; where an increased EWC was associated with a decrease in contact angle reflecting a more hydrophilic surfaces of CLs. Apart from the 3% Polysorbate 80 (p < .0001) CLs, all other formulations had light transmission values over 80%. Lenses with surfactants were found to have lower bacterial ATP concentration than lenses without surfactants. Poloxamer 188 in FSA lenses reduced bacterial adhesion from 4.22 × 10 ± 1.30 × 10 pM to 1.03 × 10 ± 4.86 × 10 pM, a reduction by 75.59% when compared to the control lenses (p = .002). Moreover, 1% Tetronic 90R4 in PDMS showed a reduction by 57.17% in ATP concentration. Polysorbate 80 in FSA exhibited the least bacterial adhesion with an average bacterial ATP concentration of 3.85 × 10 ± 2.61 × 10 pM; i.e 90.88% less bacterial ATP than control lenses (p = .001). Bioluminescence studies demonstrated a decrease in Pseudomonas aeruginosa adhesion to CLs containing surfactants without impairing the optical and mechanical characteristics of the lenses.

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Section: Effect Of Poloxamer 188 On Bacterial Adhesion To Clssupporting

confidence: 79%

Section: Effect Of Poloxamer 188 On Bacterial Adhesion To Clsmentioning

confidence: 93%

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Adherence of Pseudomonas aeruginosa onto surfactant-laden contact lenses

Mosuela

Mustafa

Gould

et al. 2018

Colloids and Surfaces B: Biointerfaces

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“…There have been no clinical reports or in vivo studies published to date reporting complications or infections with the use of the polymer material evaluated in this study. One in vitro study involving one of the component polymers (poloxamer 188) showed coating silicone wafers with the polymer reduced bacterial adhesion and was more effective than iodine in reducing Staphylococcus epidermidis colony counts on silicone surfaces [ 25 ]. In our study, the use of the polymer material considerably reduced the infection rate compared with the use of bone wax, and it had no effect on the infection rate compared with the untreated controls.…”

Section: Discussionmentioning

confidence: 99%

Infection Rates and Healing Using Bone Wax and a Soluble Polymer Material

Wellisz

Wen

et al. 2008

Clinical Orthopaedics &Amp; Related Research

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The effects of using a newly available watersoluble polymer bone hemostatic material in a contaminated environment were assessed in a rabbit tibial defect model. Infection rates and healing of polymer-treated bone were compared with the infection and healing of bone waxtreated bone and untreated controls after a bacterial challenge. Defects created in 24 rabbit tibias were treated with the polymer or bone wax, or left without a hemostatic agent. The defects were inoculated with Staphylococcus aureus ATCC-29213 (2.5 · 10 4 colony-forming units). After 4 weeks, all defects treated with bone wax were infected and osteomyelitis had developed, and none had evidence of bone healing. In the polymer and control groups, two defects in each group (25%) had osteomyelitis develop. The remaining six defects in each group (75%) showed no osteomyelitis and exhibited normal bone healing. The polymer-treated defects had a considerably lower rate of osteomyelitis and positive bone cultures compared with the bone wax-treated group. There were no differences between the polymer-treated and control groups in the rates of osteomyelitis, positive cultures, or bone healing. The use of a soluble polymer as an alternative to bone wax may decrease the rates of postoperative bone infections.

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“…Compared with negatively charged materials, positively charged materials encourage bacterial attachment but inhibit the growth of attached gram-negative bacterial cells (58,59,163). Coating surfaces with antimicrobial agents (94,165), metals (30, 89,155), photocatalytically active metal oxides (97), and surfactants (32,96,176) also has been proposed as a means to control bacterial attachment (Table 1).…”

Section: Control Of the Attachment Of Campylobacter To Surfacesmentioning

confidence: 99%

Role of Attachment to Surfaces on the Prevalence and Survival of Campylobacter through Food Systems

Nguyen

Fegan

Turner

et al. 2012

Journal of Food Protection

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Campylobacter continues to be one of the bacterial pathogens most frequently associated with human gastrointestinal illness worldwide. Because Campylobacter primarily colonizes the intestines of animals used for food production, food products of animal origin can become contaminated with this pathogen and thus represent a significant risk factor. Despite application of numerous physical and chemical interventions to control Campylobacter during food processing, the high isolation rate of this pathogen from some retail meat products indicates that Campylobacter is able to persist from animal slaughterhouses through food systems. Given the fastidious growth requirements and high susceptibility of this pathogen to environmental conditions, the ability of Campylobacter to attach to food and food-related surfaces is likely to play an important role in food contamination and movement through food systems. This review was compiled to (i) describe how the attachment of Campylobacter to surfaces influences the prevalence and survival of the organism through food systems, (ii) examine the potential factors affecting the ability of Campylobacter to attach to surfaces, and (iii) suggest strategies for controlling this attachment process.

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Bacterial Adhesion to Surfactant-modified Silicone Surfaces

Cited by 12 publications

References 5 publications

Adherence of Pseudomonas aeruginosa onto surfactant-laden contact lenses

Adherence of Pseudomonas aeruginosa onto surfactant-laden contact lenses

Infection Rates and Healing Using Bone Wax and a Soluble Polymer Material

Role of Attachment to Surfaces on the Prevalence and Survival of Campylobacter through Food Systems

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