Bacterial and Archaeal Communities within an Ultraoligotrophic, High-altitude Lake in the Pre-Himalayas of the Qinghai-Tibet Plateau

Wang, Rong; Han, Rui; Long, Qifu; Gao, Xiang; Xing, Jiangwa; Shen, Guoping

doi:10.1007/s12088-020-00881-8

Cited by 6 publications

(4 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Meanwhile, PCA suggested that uncultured Woesearchaeota AR16 increased with N fertilization. While these uncultured genera are much less studied, Woesearchaeota AR16 is found to have some association with soil pH and nitrogen level, consistent with its preference for N fertilization [98]. This study used the universal primer that targets 16S rRNA of both bacteria and archaea because of its common use.…”

Section: Archaeal Indicatorsmentioning

confidence: 96%

High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monocultures

et al. 2022

View full text Add to dashboard Cite

Cover cropping (CC) is the most promising in-field practice to improve soil health and mitigate N losses from fertilizer use. Although the soil microbiota play essential roles in soil health, their response to CC has not been well characterized by bioindicators of high taxonomic resolution within typical agricultural systems. Our objective was to fill this knowledge gap with genus-level indicators for corn [Zea mays L.] monocultures with three N fertilizer rates (N0, N202, N269; kg N ha−1), after introducing a CC mixture of cereal rye [Secale cereale L.] and hairy vetch [Vicia villosa Roth.], using winter fallows (BF) as controls. A 3 × 2 split-plot arrangement of N rates and CC treatments was studied in a randomized complete block design with three replicates over two years. Bacterial and archaeal 16S rRNA and fungal ITS regions were sequenced with Illumina MiSeq system. Overall, our high-resolution bioindicators were able to represent specific functional or ecological shifts within the microbial community. The abundances of indicators representing acidophiles, nitrifiers, and denitrifiers increased with N fertilization, while those of heterotrophic nitrifiers, nitrite oxidizers, and complete denitrifiers increased with N0. Introducing CC decreased soil nitrate levels by up to 50% across N rates, and CC biomass increased by 73% with N fertilization. CC promoted indicators of diverse functions and niches, including N-fixers, nitrite reducers, and mycorrhizae, while only two N-cycling genera were associated with BF. Thus, CC can enhance the soil biodiversity of simplified cropping systems and reduce nitrate leaching, but might increase the risk of nitrous oxide emission without proper nutrient management. This primary information is the first of its kind in this system and provided valuable insights into the limits and potential of CC as a strategy to improve soil health.

show abstract

Section: Archaeal Indicatorsmentioning

confidence: 96%

High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monocultures

et al. 2022

View full text Add to dashboard Cite

show abstract

“…4), which were also found to be dominant phyla in other areas in QTP such as Qaidam Basin [16] and Qinghai Lake [71]. As the dominant bacteria we found in this study, Proteobacteria plays an essential role in various energy metabolism in QTP [16,72], and at the class level of the Proteobacteria, Alphaproteobacteria was the most prevalent in Bamucuo microbial community, with the most common genus belonging to Sphingomonas (22.4%) in the soil microbial community (Table S4c) and Yoonia (22.9%) in the water microbial community (Table S8c); however, a study on Lake Meyghan reported that Gammaproteobacteria is the most abundant class of Proteobacteria [73]. We believed that this is a unique feature of the microbial composition in Bamucuo because of the differences in environmental conditions (such as altitude).…”

Section: Microbial Community Structurementioning

confidence: 55%

Metagenomics Revealing Molecular Profiling of Microbial Community Structure and Metabolic Capacity In Bamucuo, Tibet

Wei

Sun

Yuan

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: The Qinghai-Tibet Plateau (QTP) is the highest plateau in the world, and the microorganisms there play vital ecological roles in the global biogeochemical cycle; however, detailed information on the microbial communities in QTP is still lacking. Results: Here, we performed a landscape survey of the microorganisms in Bamucuo, Tibet, resulting in 160,212 (soil) and 135,994 (water) contigs by shotgun metagenomic methods, and generated 75 nearly complete metagenome-assembled genomes (MAGs). Proteobacteria, Actinobacteria and Firmicutes were found to be the three most dominant bacterial phyla, while Euryarchaeota was the most dominant archaeal phylum. Surprisingly, Pandoravirus salinus was found in the soil microbial community. The KEGG annotations showed that the genes related to metabolism accounted for 62.9% (soil) and 58.4% (water), respectively. Of the 75 MAGs, 63 were found to contain the genes related to all the six carbon fixation pathways, and the heavy metal resistance genes, the pressure response subsystem, and the secondary metabolite biosynthesis gene clusters (BGCs) were discovered to be abundant. Conclusions: We concluded that the microorganisms in Bamucuo fix carbon mainly through the 3-hydroxypropionic bi-cycle pathway; this study, for the first time, characterized the microbial community composition and metabolic capacity in QTP high-altitude areas (with an altitude of 4,555 meters), confirmed that QTP is a huge and valuable resource bank in which more new non-resistant antibiotics and many other bioactive substances could be developed, and provided the expanded information for further microbial community studies in QTP.

show abstract

“…5), which are similar to that in Qaidam Basin in QTP (14) and Qinghai Lake (32). Proteobacteria plays an essential role in various energy metabolism in QTP (14, 33). Actinobacteria is the main source of new antibiotics and bioactive molecular candidates (34).…”

Section: Discussionmentioning

confidence: 99%

“…3b). We found that among the biological processes, the top three GO terms were cellular process (33,656), metabolic process (32,600) and response to stimulus (8,678); among the molecular functions, the top three were catalytic activity (27,005), binding (17,692) and structural molecule activity (3,089); and among the cellular components, the top three were cell (33,716), cell part (33,716) and membrane (14,342). Whereas in the water sample, biological process (35.4%) was the dominant category, followed by molecular function (32.7%) and cellular component (31.9%) (Fig.…”

Section: Introductionmentioning

confidence: 99%

Metagenomics revealing molecular profiling of microbial community structure and metabolic capacity in Bamucuo, Tibet

Peng¹,

Wei²,

Sun

et al. 2022

Preprint

View full text Add to dashboard Cite

We performed a survey of the microorganisms in Bamucuo, Tibet, resulting in 160,212 (soil) and 135,994 (water) contigs by shotgun metagenomic methods. We discovered 74 new bacterial species and reconstructed their draft genomes, which were obtained from the 75 reconstructed almost complete metagenomic assembly genomes (MAG) in the soil and water samples. Proteobacteria and Actinobacteria were found to be the most dominant bacterial phyla, while Euryarchaeota was the most dominant archaeal phylum. To our surprise, Pandoravirus salinus was found in the soil microbial community. We concluded that the microorganisms in Bamucuo fix carbon mainly through the 3-hydroxypropionic bi-cycle pathway.

show abstract

Bacterial and Archaeal Communities within an Ultraoligotrophic, High-altitude Lake in the Pre-Himalayas of the Qinghai-Tibet Plateau

Cited by 6 publications

References 48 publications

High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monocultures

High-Resolution Indicators of Soil Microbial Responses to N Fertilization and Cover Cropping in Corn Monocultures

Metagenomics Revealing Molecular Profiling of Microbial Community Structure and Metabolic Capacity In Bamucuo, Tibet

Metagenomics revealing molecular profiling of microbial community structure and metabolic capacity in Bamucuo, Tibet

Contact Info

Product

Resources

About