Bacterial and eukaryotic communities in pond water of whiteleg shrimp Litopenaeus vannamei and the bacterial communities of their stomach and midgut

Imaizumi, Kentaro; Molex, Wanlapha; Jitnavee, Chakrit; Direkbusarakom, Sataporn; Kondo, Hidehiro; Hirono, Ikuo

doi:10.1016/j.aquaculture.2022.738139

Cited by 14 publications

(4 citation statements)

References 47 publications

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“…Vibrio bacteria, such as VP AHPND and Vibrio penaeicida , colonize the shrimp’s stomach during the initial stages of infection [ 19 , 20 ]. Moreover, in contrast to mammalian animals, shrimp stomachs host a diverse range of bacteria [ 21 , 22 ], with variations observed in the presence or absence of AHPND development [ 1 , 23 ]. This knowledge suggests that the bacterial community in the stomach microbiota of shrimp, similar to the intestinal microbiota of mammals, might confer beneficial effects on shrimp.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Bacillus spp. as Potent Probiotics with Reduction in AHPND-Related Mortality and Facilitating Growth Performance of Pacific White Shrimp (Litopenaeus vannamei) Farms

Proespraiwong,

Mavichak,

Imaizumi

et al. 2023

Microorganisms

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Acute hepatopancreatic necrosis disease (AHPND) is a serious bacterial disease affecting shrimp aquaculture worldwide. In this study, natural microbes were used in disease prevention and control. Probiotics derived from Bacillus spp. were isolated from the stomachs of AHPND-surviving Pacific white shrimp Litopenaeus vannamei (22 isolates) and mangrove forest soil near the shrimp farms (10 isolates). Bacillus spp. were genetically identified and characterized based on the availability of antimicrobial peptide (AMP)-related genes. The phenotypic characterization of all Bacillus spp. was determined based on their capability to inhibit AHPND-causing strains of Vibrio parahaemolyticus (VPAHPND). The results showed that Bacillus spp. without AMP-related genes were incapable of inhibiting VPAHPND in vitro, while other Bacillus spp. harboring at least two AMP-related genes exhibited diverse inhibition activities. Interestingly, K3 [B. subtilis (srfAA+ and bacA+)], isolated from shrimp, exerted remarkable inhibition against VPAHPND (80% survival) in Pacific white shrimp and maintained a reduction in shrimp mortality within different ranges of salinity (75–95% survival). Moreover, with different strains of VPAHPND, B. subtilis (K3) showed outstanding protection, and the survival rate of shrimp remained stable among the tested groups (80–95% survival). Thus, B. subtilis (K3) was further used to determine its efficiency in shrimp farms in different locations of Vietnam. Lower disease occurrences (2 ponds out of 30 ponds) and greater production efficiency were noticeable in the B. subtilis (K3)-treated farms. Taking the results of this study together, the heat-shock isolation and genotypic-phenotypic characterization of Bacillus spp. enable the selection of probiotics that control AHPND in Pacific white shrimp. Consequently, greater disease prevention and growth performance were affirmed to be beneficial in the use of these probiotics in shrimp cultivation, which will sustain shrimp aquaculture and be environmentally friendly.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Bacillus spp. as Potent Probiotics with Reduction in AHPND-Related Mortality and Facilitating Growth Performance of Pacific White Shrimp (Litopenaeus vannamei) Farms

Proespraiwong,

Mavichak,

Imaizumi

et al. 2023

Microorganisms

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“…Studies reporting how certain bacterial species inhabiting rearing ponds affect the survival of Pacific white shrimps have been performed by several authors, as the characteristics and dynamics of microorganisms surrounding shrimp are important factors (Imaizumi et al 2022). A study by Nguyen Thi Truc et al (2021), for instance, described a strong correlation between the presence of Lactobacillus spp.…”

Section: Introductionmentioning

confidence: 99%

The structure, composition, and predicted microbiome functional genes in Pacific white shrimp (Litopenaeus vannamei) grow-out ponds with different survival rates through high-throughput sequencing

et al. 2023

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Microbiomes inhabiting rearing ponds have been frequently associated with the survival rate of cultured animals. However, specific studies reporting on the structure, composition, and functional genes of bacteria in commercial ponds with different survival rates are still scarce. Thus, using high-throughput sequencing, the present study addressed this gap by investigating microbiomes in commercial ponds with different survival rates of Pacific white shrimp, Litopenaeus vannamei. Water samples were collected from ponds with low survival rates (LSR) and ponds with high survival rates (HSR) and profiled by high-throughput sequencing of the 16S rRNA gene. Then, functional genes of microbiomes were predicted using PICRUSt (Phylogenetic Investigation of Communities by Reconstruction of Unobserved States) software. The results showed that ponds with different survival rates had distinct bacterial communities. At the genus level, 20 genera had different relative abundance in the two pond groups (LDA > 3), of which 11 genera were in HSR ponds and nine genera were in LSR ponds. The top five most abundant bacterial genera found in LSR ponds were Vibrio, Kocuria, Tepidiphilus, unidentified_Alphaproteobacteria, and Pseudoalteromonas, while the five most abundant found in the HSR ponds were Candidatus_Aquiluna, unidentified_Acinobacteria, Ilumatobacter, unidentified_Deltaproteobacteria, and Marivita. PICRUSt analysis revealed that nine metabolic pathways were higher in HSR grow-out ponds and associated with carbohydrate metabolism, chlorophyll biosynthesis, lignin degradation, arginine and glucosamine biosynthesis, and vitamin K2 synthesis, while nine metabolic pathways were found to be significantly higher in LSR grow-out ponds including lipid IVA biosynthesis (conserved structure in diverse Gram-negative pathogens), and L-tryptophan degradation activity (proteolytic agents). To the authors' knowledge, this is the first study to report the diversity, composition, and metabolic activity of microbiomes in commercial shrimp grow-out ponds and their effect on the survival rate of Pacific white shrimp. Overall, these findings suggest that the specific microbiomes in rearing water may be linked to the impact on improved survival of Pacific white shrimp.

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“…The whiteleg shrimp (Litopenaeus vannamei) was chosen in the present study because it is an important food source in Thailand (Imaizumi et al, 2022;. The head of the shrimp was removed.…”

Section: Preparation Of Whiteleg Shrimpmentioning

confidence: 99%

“…Whiteleg shrimp (Litopenaeus vannamei) is a valuable economic animal that is in high demand as a food source in Thailand and many countries (Imaizumi et al, 2022;Zhang et al, 2015) . However, whiteleg shrimp has a short shelf life and its quality gradually decreases during the process of post-harvest, storage, and transportation.…”

Section: Introductionmentioning

confidence: 99%

Foam-mat-freeze dried clove essential oil nanoemulsion for inhibition of spoilage microorganisms in whiteleg shrimp Litopenaeus vannamei

Pilong

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This research developed foam-mat freeze-dried clove essential oil nanoemulsion (FFD-CN) for inhibiting food spoilage microorganisms. This research comprised three parts. Firstly, the fabrication of clove essential oil nanoemulsion (CN) was optimized.� The characteristics including particle size, polydispersity index (PDI), Zeta-potential, and viscosity of the fabricated nanoemulsions were observed.�� The optimal CN having a droplet size of 30.76 nm, PDI of 0.179, Zeta-potential of -50.01 mV, and viscosity of 7.81 cP was achieved at 1% v/v clove oil and 3% v/v Tween? 80 concentration and 10,000 psi operating pressure. The CN exhibited stability against particle aggregation and phase separation throughout storage. Moreover, the CN exhibited better antimicrobial activity against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa when compared to clove oil. Secondary, the foam-mat freeze-drying (FFD) technique was used to maintain stability and antimicrobial activity of CN.� The optimal condition was found at 3% (v/v) of foaming agent (MethocelTM), 60 ?C drying temperature, and 72 h drying time. The obtained FFD-CN had the droplet size of 26.14 nm, PDI of 0.193, water activity (aw) of 0.273, solubility of 88.71%, and viscosity of 12.85 cP.� The FFD-CN exhibited good stability and maintained antimicrobial activity, as analyzed using minimum inhibitory concentration (MIC), against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus better than CN. Lastly, the FFD-CN was investigated for its efficiency to maintain the quality of whiteleg shrimp during chilled storage (4 ? 2 ?C) in comparison to CN and distilled water (control). The CN and FFD-CN were found to be effective in inhibiting the growth of bacteria in whiteleg shrimp. FFD-CN was more effective than CN in delaying the changes in trimethylamine (TMA), total volatile basic nitrogen (TVB-N), total viable count (TVC), texture, color, and pH. The analysis of volatile compounds of all samples showed that FFD-CN could maintain a higher concentration of eugenol than CN and clove oil. The results also suggested that Control, CN, and FFD-CN treated shrimp had the shelf life of 6, 8, and 10 days, respectively. The FFD-CN can effectively be used as a natural preservative for maintaining the quality and shelf life of whiteleg shrimp.

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Bacterial and eukaryotic communities in pond water of whiteleg shrimp Litopenaeus vannamei and the bacterial communities of their stomach and midgut

Cited by 14 publications

References 47 publications

Evaluation of Bacillus spp. as Potent Probiotics with Reduction in AHPND-Related Mortality and Facilitating Growth Performance of Pacific White Shrimp (Litopenaeus vannamei) Farms

Evaluation of Bacillus spp. as Potent Probiotics with Reduction in AHPND-Related Mortality and Facilitating Growth Performance of Pacific White Shrimp (Litopenaeus vannamei) Farms

The structure, composition, and predicted microbiome functional genes in Pacific white shrimp (Litopenaeus vannamei) grow-out ponds with different survival rates through high-throughput sequencing

Foam-mat-freeze dried clove essential oil nanoemulsion for inhibition of spoilage microorganisms in whiteleg shrimp Litopenaeus vannamei

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