Bacterial and Fungal Aerosols in Rural Nursery Schools in Southern Poland

Brągoszewska, Ewa; Mainka, Anna; Pastuszka, Józef S.

doi:10.3390/atmos7110142

Cited by 36 publications

(54 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, exposure to ambient bacteria has potential adverse effects on human health during both spring and winter. Furthermore, the present results suggest that the respirable fraction of the bacteria in ambient air is comparable to those obtained in indoor environments [12,15,17,29].…”

Section: The Size Distribution Of Bacterial Aerosolssupporting

confidence: 83%

See 1 more Smart Citation

Concentration and Size Distribution of Culturable Bacteria in Ambient Air during Spring and Winter in Gliwice: A Typical Urban Area

2017

Self Cite

View full text Add to dashboard Cite

Abstract:The concentrations and size distributions of culturable bacterial aerosols were measured during spring and winter in outdoor air in Gliwice, Upper Silesia, Poland. This research on culturable bacteria was carried over a period of two years. The samples were collected using a six-stage Andersen cascade impactor (with aerodynamic cut-off diameters of 7.0, 4.7, 3.3, 2.1, 1.1, and 0.65 µm). The results showed that the average concentration of culturable bacterial aerosol was 355 CFU m −3 in spring, which was four times higher than during winter (65 CFU m −3 ). Bacterial aerosol concentrations showed the unimodal size distribution with the highest range of 3.3-4.7 µm particles. The seasonal distributions of bacterial aerosol grain clearly indicate that, in winter, the size distribution of particles <7 µm is more "flattened" and is characterized by an increased share of fine fractions and a decreased share of coarse ones. Environmental parameters, such as temperature, UV radiation, relative humidity, wind velocity, as well as PM 10 and PM 2.5 concentrations, were measured in order to analyse whether environmental factors had any effect on bacterial aerosols. Statistically, the most important meteorological factors in the viability of airborne bacteria were temperature and UV radiation.

show abstract

Section: The Size Distribution Of Bacterial Aerosolssupporting

confidence: 83%

“…The quality control was performed by using PN-EN12322 [37] and ISO 11133 [38] standards, with the same operation details as in our previous studies [17,23].…”

Section: Sampling and Analysis Methodsmentioning

confidence: 99%

Concentration and Size Distribution of Culturable Bacteria in Ambient Air during Spring and Winter in Gliwice: A Typical Urban Area

2017

Self Cite

View full text Add to dashboard Cite

show abstract

“…Spring was selected because it typically has the highest concentration of bioaerosols. The studies presenting seasonal fluctuations of bioaerosol concentrations, the size distribution of bacterial aerosols and their relationship with different meteorological factors [34,35] as well as our studies performed in the Upper Silesia Region indicate the highest concentration of airborne bacteria aerosols during spring [36][37][38]. In winter, extreme conditions such as low temperatures and the heaviest rainfall and snowfall of the year result in the decrease of bacteria levels.…”

Section: Sampling and Analysis Methodsmentioning

confidence: 67%

“…Quality control was performed by using PN-EN12322 [40] and ISO 11133 [41] standards, with the same operation details as in our previous studies [37,42].…”

Section: Laboratory Analysismentioning

confidence: 99%

Assessment of Bacterial Aerosol in a Preschool, Primary School and High School in Poland

et al. 2018

Self Cite

View full text Add to dashboard Cite

Abstract:The issue of healthy educational buildings is a global concern because children are particularly at risk of lung damage and infection caused by poor indoor air quality (IAQ). This article presents the results of a preliminary study of the concentration and size distribution of bacterial aerosol in three educational buildings: a preschool, primary school, and high school. Sampling was undertaken in the classrooms with an Andersen six-stage impactor (with aerodynamic cut-off diameters of 7.0, 4.7, 3.3, 2.1, 1.1 and 0.65 µm) during spring 2016 and 2017, as well as the outside of the buildings. After incubation, bioaerosol particles captured on nutrient media on Petri dishes were quantitatively evaluated and qualitatively identified. The highest average concentration of bacterial aerosol was inside the primary school building (2205 CFU/m 3 ), whereas the lowest average concentration of indoor culturable bacteria was observed in the high school building (391 CFU/m 3 ). Using the obtained data, the exposure dose (ED) of the bacterial aerosol was estimated for children attending each educational level. The most frequently occurring species in the sampled bacterial aerosol were Gram-positive cocci in the indoor environment and Gram-positive rod-forming endospores in the outdoor environment.

show abstract

“…Laboratory quality control (QC) was practiced during the studies to minimize the risk of error. A detailed description of QC can be found in Brągoszewska et al 2016a, Brągoszewska et al 2016b, and Brągoszewska et al 2018.…”

Section: Measurement Point (Sky View)mentioning

confidence: 99%

Microbiological indoor air quality in an office building in Gliwice, Poland: analysis of the case study

Brągoszewska

Biedroń

Kozielska

et al. 2018

Air Qual Atmos Health

Self Cite

View full text Add to dashboard Cite

Bioaerosols play a significant role in indoor air quality (IAQ) as they can be the cause of several health problems, including acute allergies and infectious diseases. This study aimed to characterize and compare the microbial air quality of air-conditioned (AC) and naturally ventilated (NV) office rooms in the Upper Silesia region of Poland. The bacterial samples were collected during the late spring season. Culturable bacteria were deposited on the nutrient media on Petri dishes to investigate the viable-culturable count (VCC) of bacteria and bacterial community structure using a Biolog GEN III system. In total, 12 species of bacteria were identified, with the most isolated Macrococcus equipercicus, Micrococcus luteus D, Staphylococcus xylosus (indoor), and Bacillus species (outdoor). The indoor mean concentrations of bacterial aerosol ranged from 10 2 to 10 3 CFU m −3 , below Polish proposals for threshold limit standards in office buildings. The indoor-to-outdoor (I/O) ratios indicated that studied air pollutants in the office rooms originated from the indoor air. These results, together with community composition of bacteria, indicate that most of the bacteria present in the studied office building were relatively fresh and of human origin. Multi-antibiotic resistance (MAR) tests showed that the most antibiotic-resistant features were present in Macrococcus species. The office building exposure dose (OBED) and mass median aerodynamic diameter (MMAD) of bacteria aerosol were estimated. The highest value of OBED over the study period was obtained for staff working in offices with natural ventilation (141 CFU kg −1 ), in contrast to the value for staff working in offices equipped with air conditioning (about 100 CFU kg −1 ). The MMAD of viable airborne bacteria was higher in AC offices (2.4 μm) than in NV offices (2.2 μm).

show abstract

Bacterial and Fungal Aerosols in Rural Nursery Schools in Southern Poland

Cited by 36 publications

References 42 publications

Concentration and Size Distribution of Culturable Bacteria in Ambient Air during Spring and Winter in Gliwice: A Typical Urban Area

Concentration and Size Distribution of Culturable Bacteria in Ambient Air during Spring and Winter in Gliwice: A Typical Urban Area

Assessment of Bacterial Aerosol in a Preschool, Primary School and High School in Poland

Microbiological indoor air quality in an office building in Gliwice, Poland: analysis of the case study

Contact Info

Product

Resources

About