Bacterial and fungal disinfection via ozonation in air

Epelle, Emmanuel I.; MacFarlane, Andrew; Cusack, Michael; Burns, Anthony; Thissera, Bathini; Mackay, William G.; Rateb, Mostafa E.; Yaseen, Mohammed

doi:10.1016/j.mimet.2022.106431

Cited by 37 publications

(27 citation statements)

References 29 publications

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“…Contaminated fabric swatches can be attached to the shown platform, allowing for good contact between the generated ozone gas and the fabric's fibers. Further details of the unit are documented in the study of Epelle et al 2 The relative humidity in the chamber was 50 ± 2%. 2.3.…”

Section: Methodsmentioning

confidence: 99%

“…Fabric swatches (35% cotton and 65% polyester) were utilized as the substrates for the evaluation of ozone’s disinfection efficacy in this study. Sterile swatches were inoculated with 100 μL of the bacterial (1 × 10 8 CFU/mL) and fungal suspensions, which were prepared according to the protocol described in Epelle et al; , however, the substrates were treated in their wet conditions after inoculation. Dipslides (Figure ) were subsequently applied to the swatches and incubated (at 37 °C for 24–48 h) to evaluate the growth level, pre- and post-ozone treatment.…”

Section: Methodsmentioning

confidence: 99%

“…Experimental setup for (a) gaseous ozone disinfection (Copyright permission from Elsevier. Adapted from Figure 1 in Epelle et al) and (b) aqueous ozone disinfection .…”

Section: Methodsmentioning

confidence: 99%

“…Experimental setup for (a) gaseous ozone disinfection (Copyright permission from Elsevier. Adapted from Figure 1 in Epelle et al) and (b) aqueous ozone disinfection . An accurate comparison was enabled by utilizing the same ozone dosage (ozone concentration × time) and temperatures, for both gaseous and aqueous treatments.…”

Section: Methodsmentioning

confidence: 99%

“…Ozone is an inorganic molecule with powerful antimicrobial properties, attributable to its loosely bonded third oxygen atom (which readily oxidizes other molecules). Its degradative impact on the cell membrane, unsaturated lipids, vital proteins, DNA, and intracellular enzymes of microorganisms has been widely demonstrated. − …”

Section: Introductionmentioning

confidence: 99%

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Microbial Inactivation: Gaseous or Aqueous Ozonation?

Epelle

Emmerson

Nekrasova

et al. 2022

Ind. Eng. Chem. Res.

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For decades, ozone has been known to have antimicrobial properties when dissolved or generated in water and when utilized in its gaseous form on different substrates. This property (the ability to be used in air and water) makes it versatile and applicable to different industries. Although the medium of ozonation depends on the specific process requirements, some industries have the inherent flexibility of medium selection. Thus, it is important to evaluate the antimicrobial efficacy in both media at similar concentrations, an endeavor hardly reported in the literature. This study provides insights into ozone’s efficacy in air and water using two Gram-negative bacteria (Escherichia coli NTCC1290 and Pseudomonas aeruginosa NCTC10332), two Gram-positive bacteria (Staphylococcus aureus ATCC25923 and Streptococcus mutans), and two fungi (Candida albicans and Aspergillus fumigatus). For gaseous ozonation, we utilized a custom-made ozone chamber (equipped with ultraviolet lamps), whereas an electrolysis oxygen radical generator was applied for ozone generation in water. During gaseous ozonation, the contaminated substrates (fabric swatches inoculated with bacterial and fungal suspensions) were suspended in the chamber, whereas the swatches were immersed in ozonated water for aqueous ozone treatment. The stability of ozone nanobubbles and their resulting impact on the aqueous disinfection efficiency were studied via dynamic light scattering measurements. It was observed that ozone is more effective in air than in water on all tested organisms except Staphylococcus aureus. The presented findings allow for the adjustment of the treatment conditions (exposure time and concentration) for optimal decontamination, particularly when a certain medium is preferred for ozonation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Experimental setup for (a) gaseous ozone disinfection (Copyright permission from Elsevier. Adapted from Figure 1 in Epelle et al) and (b) aqueous ozone disinfection .…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Microbial Inactivation: Gaseous or Aqueous Ozonation?

Epelle

Emmerson

Nekrasova

et al. 2022

Ind. Eng. Chem. Res.

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A review of antimicrobial implications for improving indoor air quality

Jiang,

Muneeshwaran,

Liu

et al. 2024

J Mater Sci

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Good Feasibility of Ozone-Microwave Treatment as a Sterilization Technology to Extend the Edible Life of Candied Fruit as a Post-processed Fresh Fruit Product

Huang,

Ni,

Xie

et al. 2024

Food Bioprocess Technol

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In order to solve the problem of infestation of fresh fruit after making them into candied fruit and the quality degradation caused by the infestation, a combined ozone-microwave sterilization method was used to treat four candied fruit molds.Response surface optimization analysis showed that when the ozone infusion time was at 10 min and with 560 W microwave power for 20 s, the inhibition rates of the four candied fruit molds S1, S2, X1, and H1 were 100%, 94.28%, 100%, and 100%, respectively. Moreover, according to the characterization of morphology, DNA electrophoretic profiles, and nucleic acid/protein leakage test, the integrity of the mold cell membrane was destroyed after the combined ozone-microwave treatment because of the thermal and nonthermal effects of microwave and the strongly oxidizing properties of ozone. The molds were dead because of the efflux of intracellular nucleic acid and protein and the degradation of DNA, which influenced normal cellular metabolism. Quality identification showed a better expected quality of candied fruit.

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Bacterial and fungal disinfection via ozonation in air

Cited by 37 publications

References 29 publications

Microbial Inactivation: Gaseous or Aqueous Ozonation?

Microbial Inactivation: Gaseous or Aqueous Ozonation?

A review of antimicrobial implications for improving indoor air quality

Good Feasibility of Ozone-Microwave Treatment as a Sterilization Technology to Extend the Edible Life of Candied Fruit as a Post-processed Fresh Fruit Product

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