Bacterial antagonists and hexanal-induced systemic resistance of mango fruits against <i>Lasiodiplodia theobromae</i> causing stem-end rot

Seethapathy, Parthasarathy; Thiribhuvanamala, G.; Subramanian, K. S.; Kuppusamy, Prabakar

doi:10.1080/17429145.2016.1252068

Cited by 28 publications

(14 citation statements)

References 57 publications

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“…Previous studies have used similar “bioassay” approach as a practical step in the selection of BCAs for major pathogens and pests on crops. In assays carried out in vitro as well as on the carrot roots or mango fruits, the BCAs used were found to be capable of almost complete inhibition of P. coloratum or L. theobromae , respectively (El-Tarabily et al, 1997 ; Seethapathy et al, 2016 ). Seethapathy et al ( 2016 ) have demonstrated that dual culture technique of bacterial anatogonists using Pseudomonas fluorescens (Pf1) and Bacillus subtilis (EPCO16) reduced the pathogen population in vitro ; and further strengthened the cell-wall structures of mango fruits against L. theobromae infection.…”

Section: Discussionmentioning

confidence: 99%

“…In that regard, efforts toward using natural enemies native to the same environment can effectively reduce or exterminate pathogen populations (AbuQamar et al, 2017 ; Syed Ab Rahman et al, 2018 ). In vitro and field studies were previously applied against L. theobromae using species of Bacillus to control seed and seedling rot of bottle gourd (Sultana and Ghaffar, 2010 ), Trichoderma and Aspergillus to control inflorescence blight of cashew (Adeniyi et al, 2013 ), and Pseudomonas to control stem-end rot on mango fruits (Seethapathy et al, 2016 ). No information exists on potential antagonistic microorganisms that have been identified to be capable of managing mango dieback disease under greenhouse/field conditions caused by L. theobromae , as a component of an IPM strategy.…”

Section: Introductionmentioning

confidence: 99%

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Biological Control of Mango Dieback Disease Caused by Lasiodiplodia theobromae Using Streptomycete and Non-streptomycete Actinobacteria in the United Arab Emirates

et al. 2018

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Dieback caused by the fungus Lasiodiplodia theobromae is an important disease on mango plantations in the United Arab Emirates (UAE). In this study, 53 actinobacterial isolates were obtained from mango rhizosphere soil in the UAE, of which 35 (66%) were classified as streptomycetes (SA) and 18 (34%) as non-streptomycetes (NSA). Among these isolates, 19 (12 SA and 7 NSA) showed antagonistic activities against L. theobromae associated with either the production of diffusible antifungal metabolites, extracellular cell-wall-degrading enzymes (CWDEs), or both. Using a “novel” mango fruit bioassay, all isolates were screened in vivo for their abilities to reduce lesion severity on fruits inoculated with L. theobromae. Three isolates, two belonging to Streptomyces and one to Micromonospora spp., showed the strongest inhibitory effect against this pathogen in vitro and were therefore selected for tests on mango seedlings. Our results revealed that the antifungal action of S. samsunensis UAE1 was related to antibiosis, and the production of CWDEs (i.e., chitinase) and siderophores; whilst S. cavourensis UAE1 and M. tulbaghiae UAE1 were considered to be associated with antibiotic- and CWDE-production, respectively. Pre-inoculation in greenhouse experiments with the most promising actinobacterial isolates resulted in very high levels of disease protection in mango seedlings subsequently inoculated with the pathogen. This was evident by the dramatic reduction in the estimated disease severity indices of the mango dieback of individual biocontrol agent (BCA) applications compared with the pathogen alone, confirming their potential in the management of mango dieback disease. L. theobromae-infected mango seedlings treated with S. samsunensis showed significantly reduced number of defoliated leaves and conidia counts of L. theobromae by 2- and 4-fold, respectively, in comparison to the other two BCA applications. This indicates that the synergistic antifungal effects of S. samsunensis using multiple modes of action retarded the in planta invasion of L. theobromae. This is the first report of BCA effects against L. theobromae on mango seedlings by microbial antagonists. It is also the first report of actinobacteria naturally existing in the soils of the UAE or elsewhere that show the ability to suppress the mango dieback disease.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biological Control of Mango Dieback Disease Caused by Lasiodiplodia theobromae Using Streptomycete and Non-streptomycete Actinobacteria in the United Arab Emirates

et al. 2018

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“…They also reported that the combined treatment induces activity of defense enzymes in 'Fuerte' avocados inoculated with Lasiodiplodia theobromae and Colletotrichum gloeosporioides. In another study, dipping treatment with combination of bacterial antagonists, Bacillus subtilis and hexanal induced systemic resistance of mango fruits against Lasiodiplodia theobromae by inducing several defense-related enzymes in mango [85].…”

Section: Plant Extractsmentioning

confidence: 98%

“…and Lasiodiplodia spp. Mango [28] Hot-water treatment with benomyl followed by a prochloraz Dothiorella dominicana Mango [104] Combined Bacillus subtilis and hexanal Lasiodiplodia theobromae Mango [85] 3.7. Combined Treatments With the emergence of various fungicide-resistant isolates, and the specificity of each fungicide on the one hand, and difficulty achieving complete protection against postharvest decay using only physical or natural control on the other, one treatment alone cannot generally provide complete protection against all postharvest diseases.…”

Section: Physical Controlmentioning

confidence: 99%

Fruit Stem-End Rot

et al. 2018

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After harvest, the fruit ripens and stem-end rot (SER) starts to develop, leading to significant fruit losses. SER is caused by diverse pathogenic fungi that endophytically colonize the stem during fruit development in the orchard or field and remain quiescent until the onset of fruit ripening. During the endophytic-like stage, the pathogenic fungus colonizes the phloem and xylem of the fruit stem-end; after fruit ripening, the fungus converts to a necrotrophic lifestyle, while colonizing the fruit parenchyma, and causes SER. The fruit stem-end is colonized not only by pathogenic fungi, but also by various nonpathogenic endophytic microorganisms, including fungi, yeast and bacteria. However, little is known about the fruit stem-end endophytic microbiome, which could contain new and existing biocontrol agents. To control fruit SER, treatments such as ripening inhibition, harvesting with the stem, application of chemical or biological fungicides, or physical control such as heat treatments, cold storage, or exposure to light have been suggested. This review focuses on the characterization of SER pathogens, the stem-end microbiome, and different pre- and postharvest practices that could control fruit SER.

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“…A total of ten isolates of C. gloeosporoides were isolated, purified by single spore isolation technique and designed as Cg1-Cg10. The pathogenicity of the isolates was tested by pin prick method (Parthasarathy et al 2016b) and used for further studies.…”

Section: Isolation Of Colletotrichum Gloeosporioides Pathogenmentioning

confidence: 99%

Strategic eco-friendly management of post-harvest fruit rot in papaya caused by Colletotrichum gloeosporioides

Darshan

Vanitha

Venugopala

et al. 2019

Journal of Biological Control

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Papaya (Carica papaya L.) is one of the important fruits cultivated in the tropical and subtropical regions are widely prone to the post-harvest anthracnose disease. A sum of ten isolates of Colletotrichum gloeosporioides were collected and identified through morphological and molecular method. Morphological characterization of the isolates revealed a wide variation among the isolates with respect to colony colour, topography, margin, pigmentation and zonation. The ITS gene region and the specific primer, MKCgF coupled with ITS-4, which generated amplicons of size 560 bp and 380 bp respectively for C. gloeosporioides. The amplicon (560 bp) of virulent strain Cg1 was partially sequenced [MF062699]. In order to formulate eco-friendly management practices, the in vitro screening of different biocontrol agents viz., Bacillus spp., Pseudomonas spp., plant extracts and essential oils were tested against the C. gloeosporioides. Based on the in vitro efficacy, Bacillus sp. (BSP1) and cinnamon oil were selected and further tested under field conditions as pre harvest spray and after harvest as fruit dipping. The experimental results revealed that pre-harvest spray with Bacillus sp. (BSP1) (5%) + post-harvest dipping with cinnamon oil (0.1%) recorded the lowest PDI of 3.25 when compared to control (70.36) and also increased the shelf life of papaya fruits up to 14 days. Our results show that this novel methodology of use a combination of biocontrol agent as pre-harvest spray and essential oils as post-harvest fruit dipping will protect against post-harvest anthracnose of papaya and use of chemical fungicides can be avoided.

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Bacterial antagonists and hexanal-induced systemic resistance of mango fruits against Lasiodiplodia theobromae causing stem-end rot

Cited by 28 publications

References 57 publications

Biological Control of Mango Dieback Disease Caused by Lasiodiplodia theobromae Using Streptomycete and Non-streptomycete Actinobacteria in the United Arab Emirates

Biological Control of Mango Dieback Disease Caused by Lasiodiplodia theobromae Using Streptomycete and Non-streptomycete Actinobacteria in the United Arab Emirates

Fruit Stem-End Rot

Strategic eco-friendly management of post-harvest fruit rot in papaya caused by Colletotrichum gloeosporioides

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