Bacterial Behavior

Armitage, Judith P.; Scott, Kathryn A.

doi:10.1007/978-3-642-30123-0_53

Cited by 3 publications

(3 citation statements)

References 347 publications

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“…This arrangement may allow the cell to detach and swim away quickly, or the flagellum may contribute to cell motion while on the stalk. However, a single polar flagellum could not account for the helical motion, since the flagellum would propel the cell approximately parallel to the flagellar rotation, which in this case would be at an angle to the stalk (Armitage, 2006). Furthermore, cell motion is constrained by the fact that the cell is tethered to the stalk at multiple points.…”

Section: Discussionmentioning

confidence: 99%

High‐resolution 2D and 3D cryo‐TEM reveals structural adaptations of two stalk‐forming bacteria to an Fe‐oxidizing lifestyle

Comolli

Luef

Chan

2011

Environmental Microbiology

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Aerobic neutrophilic Fe-oxidizing bacteria (FeOB) thrive where oxic and iron-rich anoxic waters meet. Here, iron microbial mats are commonly developed by stalk-forming Fe-oxidizers adapted to these iron-rich gradient environments, somehow avoiding iron encrustation. Few details are known about FeOB physiology; thus, the bases of these adaptations, notably the mechanisms of interactions with iron, are poorly understood. We examined two stalked FeOB: the marine Zetaproteobacterium Mariprofundus ferrooxydans and a terrestrial Betaproteobacterium Gallionella-like organism. We used cryo-transmission electron microscopy and cryo-electron tomography to provide unprecedented ultrastructural data on intact cell-mineral systems. Both FeOB localize iron mineral formation at stalk extrusion sites, while avoiding surface and periplasmic mineralization. The M. ferrooxydans cell surface is densely covered in fibrils while the terrestrial FeOB surface is smooth, suggesting a difference in surface chemistry. Only the terrestrial FeOB exhibited a putative chemotaxis apparatus, which may be due to differences in chemotaxis mechanisms. Both FeOB have a single flagellum, which alone is insufficient to account for cell motion during iron oxidation, suggesting that stalk extrusion is a mechanism for motility. Our results delineate the physical framework of iron transformations and characterize possible structural adaptations to the iron-oxidizing lifestyle. This study shows ultrastructural similarities and differences between two distinct FeOB, setting the stage for further (e.g. genomic) comparisons that will help us understand functional differences and evolutionary history.

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Section: Discussionmentioning

confidence: 99%

High‐resolution 2D and 3D cryo‐TEM reveals structural adaptations of two stalk‐forming bacteria to an Fe‐oxidizing lifestyle

Comolli

Luef

Chan

2011

Environmental Microbiology

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“…A productive plant–bacteria interaction, either beneficial or pathogenic, is governed by the chemical signalling between the microorganism and the host, and it is also influenced by the environmental conditions. Bacteria have evolved to sense environmental stimuli such as temperature, light or humidity, but are also able to detect plant signals and move towards them reaching the entry points and/or the places where the multiplication will take place (Melotto et al ., 2008; Baker et al ., 2010; Armitage and Scott, 2013; Scharf et al ., 2016; Leonard et al ., 2017; Chagas et al ., 2018). Furthermore, the plant and the physical environment have their own microbiota that will compete the newcomer directly (production of antimicrobial compounds) and/or indirectly (consumption of available nutrients; Rosier et al ., 2016; Hassani et al ., 2018; Xin et al ., 2018; Rodriguez et al ., 2019).…”

Section: Introductionmentioning

confidence: 99%

Distinctive features of the Gac‐Rsm pathway in plant‐associated Pseudomonas

Ferreiro

Gallegos

2021

Environmental Microbiology

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This review focuses on the Gac-Rsm global regulatory pathway of plant-associated Pseudomonas from a novel perspective, analysing its differences and similarities between beneficial and phytopathogenic species and its role in plant interaction and colonization. Some technical considerations to approach the study of this system together with the questions that remain to be solved are also discussed.This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process which may lead to differences between this version and the Version of Record. Please cite this article as

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“…For example, motile cells in principle use metabolic overhead to run the flagellum motor and to retract and extend their pili . Cells also use their appendages as the sensors to detect and react to changes in local nutrient availability in their microenvironment. ,, In this context, we hypothesize that multiplexing used by appendages for sensing and motility contributed to diverse average growth rates. To test this idea, we measured the single-cell surface motility of the different appendage mutants and correlated these metrics to cell size, growth, and detachment metrics for the same corresponding cells for all the cells that we tracked (∼100 cells).…”

Section: Resultsmentioning

confidence: 99%

Evolution of Cell Size Homeostasis and Growth Rate Diversity during Initial Surface Colonization of Shewanella oneidensis

Lee¹,

Kim²,

Santos³

et al. 2016

ACS Nano

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Cell size control and homeostasis are fundamental features of bacterial metabolism. Recent work suggests that cells add a constant size between birth and division ("adder" model). However, it is not known how cell size homeostasis is influenced by the existence of heterogeneous microenvironments, such as those during biofilm formation. Shewanella oneidensis MR-1 can use diverse energy sources on a range of surfaces via extracellular electron transport (EET), which can impact growth, metabolism, and size diversity. Here, we track bacterial surface communities at single-cell resolution to show that not only do bacterial motility appendages influence the transition from two- to three-dimensional biofilm growth and control postdivisional cell fates, they strongly impact cell size homeostasis. For every generation, we find that the average growth rate for cells that stay on the surface and continue to divide (nondetaching population) and that for cells that detach before their next division (detaching population) are roughly constant. However, the growth rate distribution is narrow for the nondetaching population, but broad for the detaching population in each generation. Interestingly, the appendage deletion mutants (ΔpilA, ΔmshA-D, Δflg) have significantly broader growth rate distributions than that of the wild type for both detaching and nondetaching populations, which suggests that Shewanella appendages are important for sensing and integrating environmental inputs that contribute to size homeostasis. Moreover, our results suggest multiplexing of appendages for sensing and motility functions contributes to cell size dysregulation. These results can potentially provide a framework for generating metabolic diversity in S. oneidensis populations to optimize EET in heterogeneous environments.

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Bacterial Behavior

Cited by 3 publications

References 347 publications

High‐resolution 2D and 3D cryo‐TEM reveals structural adaptations of two stalk‐forming bacteria to an Fe‐oxidizing lifestyle

High‐resolution 2D and 3D cryo‐TEM reveals structural adaptations of two stalk‐forming bacteria to an Fe‐oxidizing lifestyle

Distinctive features of the Gac‐Rsm pathway in plant‐associated Pseudomonas

Evolution of Cell Size Homeostasis and Growth Rate Diversity during Initial Surface Colonization of Shewanella oneidensis

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