Bacterial Biofilm Inhibitors from Diospyros dendo

Hu, Jin‐Feng; Garo, Eliane; Goering, Matt G.; Pasmore, Mark; Yoo, Hye‐Dong; Esser, T. E. B; Sestrich, Jennifer; Cremin, Peadar; Hough, Grayson W.; Perrone, Peter; Lee, Yin-Shi L.; Le, Ngoc-Tram; O'Neil–Johnson, Mark; Costerton, J. William; Eldridge, Gary R.

doi:10.1021/np049600s

Cited by 66 publications

(47 citation statements)

References 20 publications

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“…Sessile bacteria show enhanced resistance to conventional antibiotics and host defenses. Within a biofilm matrix, bacteria are able to resist antibiotics at concentrations up to 1000–1500 times higher than that conventionally used [10]. …”

Section: Introductionmentioning

confidence: 99%

“…Other antibiofilm natural compounds include five ursine triterpenes and corosolic acid from Diospyros dendo and asiatic acid [10–12], ginseng aqueous extract and its constituent zingerone [13, 14], tannins from Anadenanthera colubrina , Commiphora leptophloeos , and Myracrodruon urundeuva [15] and bacterial products such as 3-indolylacetonitrile [16]. In addition, a few plant extract libraries have been used to control P. aeruginosa biofilm formation [17–19].…”

Section: Introductionmentioning

confidence: 99%

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Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus Pseudomonas aeruginosa

Marco

Piccioni

Pagiotti

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Pseudomonas aeruginosa is a common biofilm-forming bacterial pathogen implicated in lung, skin, and systemic infections. Biofilms are majorly associated with chronic lung infection, which is the most severe complication in cystic fibrosis patients characterized by drug-resistant biofilms in the bronchial mucus with zones, where reactive oxygen species concentration is increased mainly due to neutrophil activity. Aim of this work is to verify the anti-Pseudomonas property of propolis or bud poplar resins extracts. The antimicrobial activity of propolis and bud poplar resins extracts was determined by MIC and biofilm quantification. Moreover, we tested the antioxidant activity by DPPH and neutrophil oxidative burst assays. In the end, both propolis and bud poplar resins extracts were able to inhibit P. aeruginosa biofilm formation and to influence both swimming and swarming motility. Moreover, the extracts could inhibit proinflammatory cytokine production by human PBMC and showed both direct and indirect antioxidant activity. This work is the first to demonstrate that propolis and bud poplar resins extracts can influence biofilm formation of P. aeruginosa contrasting the inflammation and the oxidation state typical of chronic infection suggesting that propolis or bud poplar resins can be used along with antibiotic as adjuvant in the therapy against P. aeruginosa infections related to biofilm.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus Pseudomonas aeruginosa

Marco

Piccioni

Pagiotti

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…Corosolic acid was identified as previously described from Diospyros dendo, commonly called Gabon ebony (16). Additional quantities of corosolic acid were purchased from Chromadex (Santa Ana, CA).…”

Section: Chemicalsmentioning

confidence: 99%

“…These compounds were identified as biofilm inhibitors during the screening of the library in a high-throughput biofilm assay using 96-well microtiter plates. The biofilm inhibition ability of asiatic acid and corosolic acid analogs has been pre-viously reported by our groups (16,25). The microtiter plate assay selects for compounds that reduce the formation of biofilms but does not test for potential effects on established biofilms.…”

mentioning

confidence: 99%

Asiatic Acid and Corosolic Acid Enhance the Susceptibility of Pseudomonas aeruginosa Biofilms to Tobramycin

Garo

Eldridge

Goering

et al. 2007

Antimicrob Agents Chemother

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Asiatic acid and corosolic acid are two natural products identified as biofilm inhibitors in a biofilm inhibition assay. We evaluated the activities of these two compounds on Pseudomonas aeruginosa biofilms grown in rotating disk reactors (RDRs) in combination with tobramycin and ciprofloxacin. To determine the ruggedness of our systems, the antibiotic susceptibilities of these biofilms were assessed with tobramycin and ciprofloxacin. The biofilm bacteria produced in the RDR were shown to display remarkable tolerance to 10 g/ml of ciprofloxacin, thus mimicking the tolerance observed in recalcitrant bacterial infections. These studies further demonstrate that a nonmucoid strain of P. aeruginosa can form a biofilm that tolerates ciprofloxacin at clinically relevant concentrations. Neither asiatic acid nor corosolic acid reduced the viable cell density of P. aeruginosa biofilms. However, both compounds increased the susceptibility of biofilm bacteria to subsequent treatment with tobramycin, suggesting asiatic acid and corosolic acid to be compounds that potentiate the activity of antibiotics. A similar statistical interaction was observed between ciprofloxacin and subsequent treatment with tobramycin.

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“…Due to their ability to interact with many enzymes [1], nucleic acids and biological membranes [2], HPTAs display numerous pharmacological activities including, antitumoral [3][4][5], anti-inflammatory [6,7], hepatoprotective [8], anti-oxidant [9], anti-ulcer, anti-hypertensive [10], antidiabetic [11], antiviral [12], anti-hyperlipidemic [13], antiparasitical [7,14], inhibitor of biofilm [15] and antimicrobial activities [7,[16][17][18][19]. Isolation and identification of HPTAs are usually and essentially obtained via methanolic or chloroformic extraction [20], and to date more than 50,000 pentacyclic triterpenes have been characterized.…”

Section: Introductionmentioning

confidence: 99%

Physicochemical and thermodynamic characterization of hydroxy pentacyclic triterpenoic acid/γ-cyclodextrin inclusion complexes

Fontanay¹,

Kedzierewicz²,

Duval³

et al. 2011

J Incl Phenom Macrocycl Chem

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Hydroxy pentacyclic triterpenoic acids (HPTAs) have been described to exhibit numerous pharmacological properties. They also exhibit poor hydrosolubility, thus affecting their potential clinical interest. The association of these active substances with cyclodextrins could be employed to improve some properties such as bioavailability and activity. 1:1 Inclusion complexes of ursolic, oleanolic and betulinic acids with c-cyclodextrin were evaluated by DSC and 1 H NMR spectroscopy. The apparent formation constants (K f ) of the formed complexes were determined using RP-HPLC. Thermodynamic parameters DG8, DH8 and DS8 were calculated with temperatures ranging from 25 to 45°C to evaluate the complexation process. Finally the influence of c-CD on the HPTA water solubility was investigated by phase-solubility studies.

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Bacterial Biofilm Inhibitors from Diospyros dendo

Cited by 66 publications

References 20 publications

Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus Pseudomonas aeruginosa

Antibiofilm and Antioxidant Activity of Propolis and Bud Poplar Resins versus Pseudomonas aeruginosa

Asiatic Acid and Corosolic Acid Enhance the Susceptibility of Pseudomonas aeruginosa Biofilms to Tobramycin

Physicochemical and thermodynamic characterization of hydroxy pentacyclic triterpenoic acid/γ-cyclodextrin inclusion complexes

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