Bacterial biofilms and their resistance mechanisms: a brief look at treatment with natural agents

Nourbakhsh, Fahimeh; Nasrollahzadeh, Mahda Sadat; Tajani, Amineh Sadat; Soheili, Vahid; Hadizadeh, Farzin

doi:10.1007/s12223-022-00955-8

Cited by 24 publications

(11 citation statements)

References 157 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The formation of biofilms by bacteria is usually a form of protection. It is known that the difficult penetration of substances in these structures is a way to diminish the action of antibacterial drugs [ 70 , 71 ]. Due to their sizes and surface properties, AgNPs have been evaluated as antibiofilm agents [ 72 , 73 ].…”

Section: Resultsmentioning

confidence: 99%

Green Extracellular Synthesis of Silver Nanoparticles by Pseudomonas alloputida, Their Growth and Biofilm-Formation Inhibitory Activities and Synergic Behavior with Three Classical Antibiotics

et al. 2022

View full text Add to dashboard Cite

Bacterial resistance to antibiotics is on the rise and hinders the fight against bacterial infections, which are expected to cause millions of deaths by 2050. New antibiotics are difficult to find, so alternatives are needed. One could be metal-based drugs, such as silver nanoparticles (AgNPs). In general, chemical methods for AgNPs’ production are potentially toxic, and the physical ones expensive, while green approaches are not. In this paper, we present the green synthesis of AgNPs using two Pseudomonas alloputida B003 UAM culture broths, sampled from their exponential and stationary growth phases. AgNPs were physicochemically characterized by transmission electron microscopy (TEM), total reflection X-ray fluorescence (TXRF), infrared spectroscopy (FTIR), dynamic light scattering (DLS), and X-ray diffraction (XRD), showing differential characteristics depending on the synthesis method used. Antibacterial activity was tested in three assays, and we compared the growth and biofilm-formation inhibition of six test bacteria: Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis. We also monitored nanoparticles’ synergic behavior through the growth inhibition of E. coli and S. aureus by three classical antibiotics: ampicillin, nalidixic acid, and streptomycin. The results indicate that very good AgNP activity was obtained with particularly low MICs for the three tested strains of P. aeruginosa. A good synergistic effect on streptomycin activity was observed for all the nanoparticles. For ampicillin, a synergic effect was detected only against S. aureus. ROS production was found to be related to the AgNPs’ antibacterial activity.

show abstract

Section: Resultsmentioning

confidence: 99%

Green Extracellular Synthesis of Silver Nanoparticles by Pseudomonas alloputida, Their Growth and Biofilm-Formation Inhibitory Activities and Synergic Behavior with Three Classical Antibiotics

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Biofilms are composed of bacterial communities surrounded by a self-produced polymer matrix that protects them from diverse environmental stresses ( Nourbakhsh et al., 2022 ). eDNA is an important component of the biofilm extracellular matrix ( Rajendran et al., 2013 ), which exists in two forms, namely B-form eDNA (B-DNA) and Z-form eDNA (Z-DNA).…”

Section: Discussionmentioning

confidence: 99%

DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models

Deng

Lei

Tang

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Anti-infection strategies against pleural empyema include the use of antibiotics and drainage treatments, but bacterial eradication rates remain low. A major challenge is the formation of biofilms in the pleural cavity. DNase has antibiofilm efficacy in vitro, and intrapleural therapy with DNase is recommended to treat pleural empyema, but the relevant mechanisms remain limited. Our aim was to investigate whether DNase I inhibit the early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models. We used various assays, such as crystal violet staining, confocal laser scanning microscopy (CLSM) analysis, peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH), and scanning electron microscopy (SEM) analysis. Our results suggested that DNase I significantly inhibited early biofilm formation in a dose-dependent manner, without affecting the growth of P. aeruginosa or S. aureus in vitro. CLSM analysis confirmed that DNase I decreased the biomass and thickness of both bacterial biofilms. The PNA-FISH and SEM analyses also revealed that DNase I inhibited early (24h) biofilm formation in two empyema models. Thus, the results indicated that DNase inhibited early (24h) biofilm formation in P. aeruginosa- or S. aureus-induced rabbit empyema models and showed its therapeutic potential against empyema biofilms.

show abstract

“…However, after the endothelium is damaged by inflammation, sclerosis, and direct bacterial activity; infective endocarditis (IE) occurs with a change in the endocardium, including the platelet and microorganism-rich vegetations [58] , [59] , [60] . With the accumulation of microorganisms to thrombus, the vegetation biofilm becomes mature and causes embolization, demonstrating the stigmata of IE [61] . A biofilm is considered a cooperative microbial community that is surrounded by a self-produced extracellular matrix [62] .…”

Section: Main Textmentioning

confidence: 99%

Nanotechnology in interventional cardiology: A state-of-the-art review

Almas¹,

Haider²,

Malik³

et al. 2022

IJC Heart & Vasculature

View full text Add to dashboard Cite

Bacterial biofilms and their resistance mechanisms: a brief look at treatment with natural agents

Cited by 24 publications

References 157 publications

Green Extracellular Synthesis of Silver Nanoparticles by Pseudomonas alloputida, Their Growth and Biofilm-Formation Inhibitory Activities and Synergic Behavior with Three Classical Antibiotics

Green Extracellular Synthesis of Silver Nanoparticles by Pseudomonas alloputida, Their Growth and Biofilm-Formation Inhibitory Activities and Synergic Behavior with Three Classical Antibiotics

DNase inhibits early biofilm formation in Pseudomonas aeruginosa- or Staphylococcus aureus-induced empyema models

Nanotechnology in interventional cardiology: A state-of-the-art review

Contact Info

Product

Resources

About