Bacterial Biofilms in Surgical Specimens of Patients with Chronic Rhinosinusitis

Sanclement, Jose A.; Webster, Paul; Thomas, John G.; Ramadan, Hassan H.

doi:10.1097/01.mlg.0000161346.30752.18

Cited by 219 publications

(216 citation statements)

References 20 publications

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“…Ferguson reported a biofilm detection rate of 50 % by TEM [23]. SEM can detect the 3-D structure, glycocalyx, and water channels of biofilms, while TEM can detect evidence of bacteria [24]. In contrast to electron microscopy, Confocal Scanning Laser Microscope (CSLM) better preserves the structure of a biofilm.…”

Section: Discussionmentioning

confidence: 99%

Clinical Characteristics of Biofilms in Patients with Chronic Rhinosinusitis: A Prospective Case–Control Study

Jung

Eog

Kang

et al. 2014

Indian J Otolaryngol Head Neck Surg

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Recent studies have indicated that biofilms are involved in the pathogenesis of recurrent and recalcitrant chronic rhinosinusitis (CRS). The present study was performed to evaluate the presence of biofilms and to evaluate the relationships between the presence of biofilms and clinical features of CRS. A total of 33 patients were included in this study. Maxillary sinus mucosa from 26 CRS patients and the ethmoid mucosa from 7 patients undergoing septoplasty were collected. Biofilms were evaluated by scanning electron microscopy. Preoperative symptom scores, preoperative and intraoperative nasoendoscopic findings, and postoperative healing period were compared between the groups. Biofilms were detected in 14 (42.4 %) of the 33 patients. Biofilms were present in 13 (50 %) of the 26 patients in the CRS group, but in only one (14.3 %) of the seven patients in the control group. There were no significant differences in preoperative symptom scores or preoperative nasal Lund-Kennedy endoscopic scores between the groups. However, the average LundMackay and intraoperative sinus Lund-Kennedy endoscopic scores were significantly higher in biofilm-positive than biofilm-negative patients (P \ 0.05). In the follow-up period, the healing time was significantly longer in biofilmpositive than biofilm-negative patients (P \ 0.05). This study suggested that the presence of bacterial biofilms may contribute to the pathogenesis of CRS and the clinical characteristics of CRS patients after endoscopic sinus surgery.

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Section: Discussionmentioning

confidence: 99%

Clinical Characteristics of Biofilms in Patients with Chronic Rhinosinusitis: A Prospective Case–Control Study

Jung

Eog

Kang

et al. 2014

Indian J Otolaryngol Head Neck Surg

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“…Pneumococcal biofilms have been detected on nasal septum biopsy specimens from humans with sinusitis, on septal epithelia of experimentally challenged mice, and form in vitro on cultured respiratory epithelial cells and abiotic surfaces covered with bacterial media (13)(14)(15)(16)(17). Biofilm formation during colonization is thought to confer protection against host defenses and desiccation (18,19).…”

mentioning

confidence: 99%

Neuraminidase A-Exposed Galactose Promotes Streptococcus pneumoniae Biofilm Formation during Colonization

et al. 2016

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f Streptococcus pneumoniae is an opportunistic pathogen that colonizes the nasopharynx. Herein we show that carbon availability is distinct between the nasopharynx and bloodstream of adult humans: glucose is absent from the nasopharynx, whereas galactose is abundant. We demonstrate that pneumococcal neuraminidase A (NanA), which cleaves terminal sialic acid residues from host glycoproteins, exposed galactose on the surface of septal epithelial cells, thereby increasing its availability during colonization. We observed that S. pneumoniae mutants deficient in NanA and ␤-galactosidase A (BgaA) failed to form biofilms in vivo despite normal biofilm-forming abilities in vitro. Subsequently, we observed that glucose, sucrose, and fructose were inhibitory for biofilm formation, whereas galactose, lactose, and low concentrations of sialic acid were permissive. Together these findings suggested that the genes involved in biofilm formation were under some form of carbon catabolite repression (CCR), a regulatory network in which genes involved in the uptake and metabolism of less-preferred sugars are silenced during growth with preferred sugars. Supporting this notion, we observed that a mutant deficient in pyruvate oxidase, which converts pyruvate to acetyl-phosphate under non-CCR-inducing growth conditions, was unable to form biofilms. Subsequent comparative transcriptome sequencing (RNA-seq) analyses of planktonic and biofilm-grown pneumococci showed that metabolic pathways involving the conversion of pyruvate to acetyl-phosphate and subsequently leading to fatty acid biosynthesis were consistently upregulated during diverse biofilm growth conditions. We conclude that carbon availability in the nasopharynx impacts pneumococcal biofilm formation in vivo. Additionally, biofilm formation involves metabolic pathways not previously appreciated to play an important role.

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“…Further studies found significant differences in the rate of biofilms formation between control, CRS and refractory sinusitis (Bendouah et al, 2006;Psaltis et al, 2007;Sanclement et al, 2005).…”

Section: Biofilm and Rcrsmentioning

confidence: 89%

“…During this phase water channels are formed. Once biofilms reach maturity, bacteria slough off and embolize to other areaswhere the process may begin again (Sanclement et al,2005).…”

Section: Biofilm and Rcrsmentioning

confidence: 99%

Refractory Chronic Rhinosinusitis: Etiology & Management

Al‐Qudah¹

2011

Peculiar Aspects of Rhinosinusitis

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Bacterial Biofilms in Surgical Specimens of Patients with Chronic Rhinosinusitis

Cited by 219 publications

References 20 publications

Clinical Characteristics of Biofilms in Patients with Chronic Rhinosinusitis: A Prospective Case–Control Study

Clinical Characteristics of Biofilms in Patients with Chronic Rhinosinusitis: A Prospective Case–Control Study

Neuraminidase A-Exposed Galactose Promotes Streptococcus pneumoniae Biofilm Formation during Colonization

Refractory Chronic Rhinosinusitis: Etiology & Management

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