Bacterial blotch of melons caused by strains of Acidovorax avenae subsp. citrulli

O'Brien, R. G.; Martin, H. L.

doi:10.1071/ea98172

Cited by 60 publications

(42 citation statements)

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“…The disease started to gain importance after severe outbreaks in watermelon fields in the USA during the late 1980s/early 1990s (Wall and Santos 1988;Wall et al 1990;Somodi et al 1991). Since then, BFB has been reported in other cucurbits, such as melon, pumpkin and cucumber, and has spread to many parts of the world, including the Americas, Asia, Europe, the Middle East and Australia (Isakeit et al 1997(Isakeit et al , 1998Langston et al 1999;Martin and O'Brien 1999;O'Brien and Martin 1999;Schaad et al 2003;Walcott et al 2004;Burdman et al 2005).…”

Section: Discussionmentioning

confidence: 97%

Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity

2008

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Bacterial fruit blotch (BFB) of cucurbits, caused by Acidovorax avenae subsp. citrulli, is a serious threat to the watermelon and melon industries. To date, there are no commercial cultivars of cucurbit crops resistant to the disease. Here we assessed the level of tolerance to bacterial fruit blotch of various commercial cultivars as well as breeding and wild lines of melon, using seed-transmission assays and seedling-inoculation experiments. Selected cultivars were also tested in a greenhouse experiment with mature plants. All tested cultivars/lines were found to be susceptible to the pathogen, and most of them showed different responses (relative tolerance vs. susceptibility) in the different assays; however, some consistent trends were found: cv. ADIR339 was relatively tolerant in all tested assays, and cv. 6407 and wild lines BLB-B and EAD-B were relatively tolerant in seed-transmission assays. We also provide evidence supporting a strong correlation between the level of susceptibility of a cultivar/line and the ability of the pathogen to adhere to or penetrate the seed. To the best of our knowledge, this is the first attempt to assess melon cultivars/lines for bacterial fruit blotch response.

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Section: Discussionmentioning

confidence: 97%

Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity

2008

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“…Bacterial fruit blotch of melon by Acidovorax avenae subsp. citrulli has been reported, but it causes water-soaking with a reddish-brown discoloration on the surface and in internal tissue of fruits (Horita et al 2006;O'Brien and Martin 1999;Tomita et al 2006). None of these reports mentioned an internal rot of melon fruits caused by bacteria.…”

Section: Introductionmentioning

confidence: 96%

Internal fruit rot of netted melon caused by Pantoea ananatis (=Erwinia ananas) in Japan

Kido

Adachi²,

Hasegawa³

et al. 2008

J Gen Plant Pathol

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An internal fruit rot with a malodor was found in netted melons (Cucumis melo L.) in commercial greenhouses in Kochi Prefecture, Japan, in 1998, despite their healthy appearance and lack of water-soaking or brown spots on the surface. A yellow bacterium was consistently isolated from the affected fruits. To confirm the pathogenicity of eight representative isolates of the yellow bacterium, we stub-inoculated ovaries (immature-fruits) 5-7 days after artificial pollination, with a pin smeared with bacteria. After the melon fruits had grown for 60 more days, an internal fruit rot resembling the natural infection appeared, and the inoculated bacterium was reisolated. The melon isolates had properties identical with Pantoea ananatis, such as gram-negative staining, facultative anaerobic growth, indole production, phenylalanine deaminase absence, and acid production from melibiose, sorbitol, glycerol, and inositol. Phylogenetic analysis based on 16S rDNA sequences showed that the melon bacterium positioned closely with known P. ananatis strains. The melon bacterium had indole acetic acid (IAA) biosynthesis genes (iaaM and iaaH) and a cytokinin biosynthesis gene (etz). The bacterium could be distinguished from the other 'Pantoea' group strains by rep-PCR genomic fingerprinting. From these results, the causal agent of internal fruit rot was identified as a strain of P.ananatis [Serrano in (Philipp J Sci 36:271-305, 1928); Mergaert et al. in (Int J Syst

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“…Strains of A. avenae subsp. citrulli have been divided into at least two groups on the basis of pathogenicity on different hosts, DNA fingerprinting and other bacteriological properties including fatty acid composition: one includes strains more associated with watermelon; the other mainly consists of those isolated from nonwatermelon hosts (Burdman et al 2005;Feng et al 2009;O'Brien and Martin 1999;Walcott et al , 2004. Kawicha et al (2002) additionally reported that the subspecies has further diversity in their pathogenicity and disease severity.…”

Section: Introductionmentioning

confidence: 97%

Genetic diversity and pathogenicity of cucurbit-associated Acidovorax

Makizumi¹,

Igarashi²,

Gotoh³

et al. 2010

J Gen Plant Pathol

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Bacterial fruit blotch of cucurbits is a destructive disease caused by Acidovorax avenae subsp. citrulli, which is a typical seedborne pathogen. In seed health testing for this disease, we have detected many strains of Acidovorax with some differences from A. avenae subsp. citrulli. Their 16S rRNA sequences were divided into six types. The most common sequence was completely consistent with that of A. avenae subsp. avenae originally isolated from rice. The other sequences were over 99% similar but not identical to those of A. avenae subsp. avenae and A. avenae subsp. citrulli. Some commercialized antibodies against A. avenae subsp. citrulli reacted with several of these strains. Some of these strains incited yellow spots or brownish water-soaked lesions mainly on young true leaves of cucumber and squash after spray inoculation. Histological observations showed that these strains entered the leaf tissues of cucurbit plants through stomata and multiplied in the intercellular spaces of parenchymatous tissues as well as in the vascular tissues. The amount of bacterial multiplication and spread in the tissues differed among the strains, presumably reflecting their ability to induce symptoms. These isolated strains are therefore different from A. avenae subsp. citrulli, and their potential threat to the cultivation of cucurbits is lower than that of A. avenae subsp. citrulli.

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Bacterial blotch of melons caused by strains of Acidovorax avenae subsp. citrulli

Cited by 60 publications

References 0 publications

Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity

Bacterial fruit blotch of melon: screens for disease tolerance and role of seed transmission in pathogenicity

Internal fruit rot of netted melon caused by Pantoea ananatis (=Erwinia ananas) in Japan

Genetic diversity and pathogenicity of cucurbit-associated Acidovorax

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