Bacterial cell wall material properties determine E. coli resistance to sonolysis

Abstract

Highlights Weakened or absent peptidoglycan enables efficient bacterial sonolysis. Bacteria without peptidoglycan are as sensitive to sonolysis as lipid vesicles. Remodeling capsule or outer membrane has no effect on bacterial sonolysis. Non growing bacteria are 5 fold more resistant to sonolysis than growing bacteria.

Inactivation of the enveloped virus phi6 with hydrodynamic cavitation

“…Based on the result from the IT experiments with methanol ( Table 2 : HC+M – IT30), we speculate that mechanical effects of cavitation are the reason. This finding also corresponds well with the recent experimental and numerical research on cavitation bubble interaction with liposomes [46] , [47] and bacteria [48] , [49] , where it was shown, that microstreaming is the primary mechanical driver of liposome deformation and bacterial cell damage, and that poration of cell membranes could be explained solely by mechanical effects.…”

Investigating the effect of acidic and basic precipitation on the antibacterial activity of ZnO nanoparticles against Gram-negative and Gram-positive bacteria

“…111 The E. coli cell envelope has a cytoplasmic membrane, peptidoglycan layer, outer membrane, capsular layer, and slime-layer loosely attached to extracellular polymeric substances. 112 Each layer or structure has a unique chemical composition, which in combination with the other layers, form a multi-composite cell envelope structure that resists environmental stresses. K. pneumoniae is a Gram-negative rod-shaped bacterial strain that belongs to the highly obnoxious group of bacteria called ESKAPE.…”

“…91, 34.51, 36.41, 47.61, 56.61, 62.71, 67.91, and 69.11. The observed diffraction peaks correspond to the crystal planes, ( 100), ( 002), ( 101), ( 102), ( 110), ( 103), (200), and (112). The observed X-ray diffraction pattern indicates that the synthesized ZnO NPs possess a hexagonal crystal phase with a Wurtzite structure.…”

Probiotic properties and antibiotic susceptibility assessment of Streptococcus thermophilus isolates

“…After centrifugation for 10 min at 4000 rpm at 4°C, the pellets and the supernatants were collected to evaluate their antioxidant activity separately. In this study, the ultrasonic crusher noise isolation chamber; which is designed to apply a cavitation effect in a liquid through ultrasonic waves, was used to disintegrate bacterial cells [38,39,40]. For that, the pellet containing the bacteria was resuspended in water and subjected to sonication (ultrasonic cell crusher noise isolating chamber) to burst the bacterial cells and release their intracellular medium.…”