Bacterial Cellulose Production by Fed-Batch Fermentation in Molasses Medium

Abstract

Batch and fed-batch fermentations for bacterial cellulose (BC) production using molasses as a carbon source by Acetobacter xylinum BPR2001 were carried out in a jar fermentor. For improvement of BC production, molasses was subjected to H2SO4-heat treatment. The maximum BC concentration by this treated molasses increased 76%, and the specific growth rate increased 2-fold compared with that by untreated molasses. In batch fermentation, when the initial sugar concentrations of H2SO4-heat-treated molasses were varied from 20 to 70 g/L, the highest value of maximum BC concentration of 5.3 g/L was observed at 20 g/L. BC production in intermittent fed-batch (IFB) fermentation was conducted referring to the data in batch fermentation, and the highest BC production of 7.82 g/L was obtained when 0.2 L of molasses medium was added five times. When continuous fed-batch (CFB) fermentations were conducted, maximum BC concentration was obtained with a feeding rate of 6.3 g-sugar/h, which was derived from the optimal IFB experiment.

Utilização do soro de mandioca como substrato fermentativo para a biosíntese de goma xantana: viscosidade aparente e produção

“…A fermentação de soro de leite resultou em 13,0 28,0 g.L -1 de goma xantana [19] , enquanto que de resíduos agroindustriais de café, 5,8 g.L -1 [19] ; e de resíduo do processamento de suco de maçã, 45 g.L -1 de goma xantana [20] . A produção de gelana a partir de resíduo da indústria de soja [21] , a produção de celulose bacteriana a partir do melaço de cana de açúcar pré tratado [22] e a produção de pululana utilizando hidrolisado de torta de soja [23] são outras bioconversões de resíduos agroindustriais.…”

Beneficial Effect of Acetic Acid on the Xylose Utilization and Bacterial Cellulose Production by Gluconacetobacter xylinus

“…Many factors would influence the BC productivity and fermentation substrate cost is a significant one. In order to reduce its production cost, different substrates such as molasses [2], konjac powder hydrolysate [3], various fruit juices [4] and etc. have been utilized for BC production already.…”

Bacterial Cellulose Production by Komagataeibacter hansenii ATCC 23769 Using Sisal Juice - An Agroindustry Waste

“…In another research, the highest BC production (5.3 g/L) was reached after Acetobacter xylinum BPR 2001 culture in sugar cane molasses with a sugar concentration adjusted to 20 g/L. At higher sugar concentrations (50 and 70 g/l), the production decreased (Bae and Shoda, 2004).…”

“…To reduce production costs, research projects have evaluated agroindustrial sources as alternative culture media for BC production. Examples of agro-industrial sources studied include: fruit juices (Jozala et al, 2015;Kurosumi et al, 2009), molasses (Çakar et al, 2014), molasses-corn steep liquor (Bae and Shoda, 2004;Jung et al, 2010), aqueous extract from grape skins, cheese whey, crude glycerol, sulfite pulping liquor (Jozala et al, 2015;Carreira et al, 2009), pineapple peel juice, sugar cane juice (Castro et al, 2011), coconut juice (Kongruang, 2008), waste beer yeast (Lin et al, 2014), maple syrup (Zeng et al, 2011), hydrolyzed starch, and coconut water (Hungund, 2013). In many cases, it is possible to achieve similar or superior production of BC when compared to methods using synthetic media.…”