Bacterial cellulose tubes as a nerve conduit for repairing complete facial nerve transection in a rat model

Binnetoğlu, Adem; Demir, Berat; Akakın, Dilek; Demirci, Elif; Batman, Caglar

doi:10.1007/s00405-019-05637-9

Cited by 27 publications

(16 citation statements)

References 20 publications

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“…An ideal nerve conduit should require antibacterial activity to avoid the possibility of clinical infection. Studies have shown that pure BNC in vitro and that surgically implanted in vivo lack antibacterial activity, resulting in infection and death of rats . The antibacterial ability of the CSNPs/OBC and NGF@CSNPs/OBC was investigated by using the absorption method (Figure and Table ).…”

Section: Resultssupporting

confidence: 51%

In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration

et al. 2021

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Autograft is currently the gold standard in the clinical treatment of peripheral nerve injury (PNI), which, however, is limited by the availability of a donor nerve and secondary injuries. Nerve guidance conduits (NGC) provide a suitable microenvironment to promote the regeneration of injured nerves, which could be the substitutes for autografts. In this study, nerve growth factor (NGF) encapsulated chitosan nanoparticles (CSNPs) were first constructed in situ in an oxidized bacterial cellulose (OBC) conduit using the ion gel method after the introduction of a CS/NGF solution under pressure to enable a sustainable release of NGF. A novel NGF@CSNPs/OBC nanocomposite with antibacterial activity, biodegradability, and porous microstructure was successfully developed. In vitro experiments showed that the nanocomposite promoted the adhesion and proliferation of Schwann cells. When the nanocomposite was applied as NGC to repair the sciatic nerve defect of rats, a successful repair of the 10 mm nerve defect was observed after 4 weeks. At week 9, the diameter, morphology, histology, and functional recovery of the regenerated nerve was comparable to the autografts, indicating that the NGC effectively promoted the regeneration and function recovery of the nerve. In summary, the NGF@CSNPs/OBC as a novel NGC provides great potential in the treatment of PNI.

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Section: Resultssupporting

confidence: 51%

In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration

et al. 2021

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“…The results of whisker movements and electrophysiological parameters tests confirmed that despite no improvement in facial nerve function in the presence of the BC tubes, the regenerating axons were significantly higher in number than other control methods. This observation suggests that the BC hallow structure guides the fibers from proximal to distal nerve stump, enhances axonal regeneration, and provides a nerve conduit for neural regeneration [41].…”

Section: Natural Cellulose For Other Tissue Engineering Applicationsmentioning

confidence: 93%

Recent Advances of Natural Cellulosic Non-Woven Scaffolds for Tissue Engineering

Aghazadeh¹,

Delfanian²,

Aghakhani³

et al. 2022

Preprint

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In the recent years, tissue engineering researchers have exploited a variety of biomaterials that can potentially mimic extracellular matrix (ECM) for tissue regeneration. Natural cellulose, mainly obtained from bacterial (BC) and plant-based (PC) sources, can serve as a high potential scaffold material for different regenerative purposes. Natural cellulose has drawn the attention of researchers due to its advantage over synthetic cellulose in terms of availability, cost-effectiveness, perfusablility, biocompatibility, negligible toxicity, mild immune response and due to imitating native tissues. In this article, we will review the recent in vivo and in vitro studies aimed to assess the potentials of natural cellulose for the purpose of soft (skin, heart, veins, nerve, among others) and hard (bone and tooth) tissue engineering.

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“…The use of conduits for neural repair is a common strategy where different materials and/or grafts are implanted at the injury site to guide the neuronal regeneration. 107 Different additives have been tested on the fabricated scaffolds for neuronal regeneration, such as fibroblast growth factor, 108 IL-6, neurotrophins, glial-derived neurotrophins, and persephin. 109 Most of the reported studies have used pluripotent stem cells because they are easy to harvest and readily differentiate to nerve tissue.…”

Section: Craniofacial Nerve Tissue Engineeringmentioning

confidence: 99%

Recent update on craniofacial tissue engineering

Emara

Shah²

2021

J Tissue Eng

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The craniofacial region consists of several different tissue types. These tissues are quite commonly affected by traumatic/pathologic tissue loss which has so far been traditionally treated by grafting procedures. With the complications and drawbacks of grafting procedures, the emerging field of regenerative medicine has proved potential. Tissue engineering advancements and the application in the craniofacial region is quickly gaining momentum although most research is still at early in vitro/in vivo stages. We aim to provide an overview on where research stands now in tissue engineering of craniofacial tissue; namely bone, cartilage muscle, skin, periodontal ligament, and mucosa. Abstracts and full-text English articles discussing techniques used for tissue engineering/regeneration of these tissue types were summarized in this article. The future perspectives and how current technological advancements and different material applications are enhancing tissue engineering procedures are also highlighted. Clinically, patients with craniofacial defects need hybrid reconstruction techniques to overcome the complexity of these defects. Cost-effectiveness and cost-efficiency are also required in such defects. The results of the studies covered in this review confirm the potential of craniofacial tissue engineering strategies as an alternative to avoid the problems of currently employed techniques. Furthermore, 3D printing advances may allow for fabrication of patient-specific tissue engineered constructs which should improve post-operative esthetic results of reconstruction. There are on the other hand still many challenges that clearly require further research in order to catch up with engineering of other parts of the human body.

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Bacterial cellulose tubes as a nerve conduit for repairing complete facial nerve transection in a rat model

Cited by 27 publications

References 20 publications

In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration

In Situ Fabrication of Nerve Growth Factor Encapsulated Chitosan Nanoparticles in Oxidized Bacterial Nanocellulose for Rat Sciatic Nerve Regeneration

Recent Advances of Natural Cellulosic Non-Woven Scaffolds for Tissue Engineering

Recent update on craniofacial tissue engineering

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