Bacterial chemotaxis controls the catabolite repression of flagellar biogenesis

Vorobyeva, N.V.; Sherman, Michael Y.; Glagolev, A.N.

doi:10.1016/0014-5793(82)80106-3

Cited by 7 publications

(3 citation statements)

References 13 publications

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“…However, an additional block in the MCP-dependent pathway by either applying aspartate and serine or depleting Sadomet by ethionine completely suppressed There is at least one case in which the MCP-PTS interaction has a definite adaptive role, namely: the control of flagellar synthesis. We previously found that indole and Co 2+, MCP repellents telieved glucose catabolite repression of flagellar biogenesis [21]. The repellents acted via adenylate cyclase, and from the present study it follows that the control pathway is: repellents ~ MCP's--, PTS gl~ -0 adenylate cyclase ~ cAMP ---, flagellar synthesis.…”

Section: Pts-mcp Interactionsupporting

confidence: 68%

Functional interaction between information processing pathways in Escherichia coli and the nature of Δ-H+ (redox)-sensing

Milgrom¹

1985

FEMS Microbiology Letters

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SUMMARY 2. INTRODUCTIONGlucose taxis and O2-taxis in Escherichia coil signal to flagella via a pathway that includes PTS glc and adenylate cyclase. Information from a number of attractants and repellents is focused at the level of methyl-accepting chemotaxis proteins (MCPs) and information is passed to flagella by a separate pathway. Mutants defective in adenylate cyclase (Acya) had a residual taxis to glucose that was eliminated by preincubating the cells with MCP attractants, or by depleting the -CH 3 donor. A methyltransferase mutant had a decreased sensitivity to MCP repellents and this response was completely blocked by preincubating the cells with glucose. Likewise, the response of cells, depleted for -CH3, towards repellents, was blocked if bacteria carried a pts mutation. It is concluded that PTS and MCP pathways exchange information. In cya cells, 0 2 taxis was restored in the presence of maltose, an MCPII attractant. It is suggested that MCPII is an additional protonmotive force (pmf) sensor. * A preliminary account of a part of this study has been published (Milgrom, E.M., Sherman, M.Yu. and Glagolev, A.N. (1983) Dokl. Akad. Nauk USSR 272, 473-476).In E. coli, information flows from receptors to flagella along different types of pathways. The first is dependent on MCP that focus information from several effectors and/or receptors. Amino acids, repellents (indole, valine, Co 2+, etc.) and certain sugars (maltose, ribose and galactose) signal through MCPs [1]. MCP methylation represents adaptation to constant-level attractant stimuli [2]. Substrates of phosphoenolpyruvate-dependent phosphotransferase systems (PTS) are attractants that use the respective enzymes II PTS as receptors [3,4], and signal independently of MCP [5]. The sensing of pmf and a related process of oxygen taxis also signal independent of MCP [6,7]. We have recently found that the glucose PTS is actually the pmf sensor, with the information passing to flagella via an adenylate cyclase-requiring step [8]. Adler and coworkers [9] reported that adenylate cyclase was involved in signal transmission from PTS substrates but found that a residual response could be observed in Acya cells.In studying the chemotactic properties of Salmonella typhimurium che R mutants lacking the methyl transferase. Koshland and coworkers found that these cells were nevertheless able to respond 0378-1097/85/$03.30

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Section: Pts-mcp Interactionsupporting

confidence: 68%

Functional interaction between information processing pathways in Escherichia coli and the nature of Δ-H+ (redox)-sensing

Milgrom¹

1985

FEMS Microbiology Letters

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“…A previous report suggested that the chemotaxis system itself may have a feedback effect on flagellum synthesis (48). In this study, we have also ruled out the possibility that the motility defect of A(dnaK-dnaJ) strains results from a repression signal from the chemotaxis machinery.…”

Section: Resultsmentioning

confidence: 70%

DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli

et al. 1992

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The DnaK, DnaJ, and GrpE heat shock proteins are required for motility of Escherichia coli. Cells deleted for dnaK or dnaJ, or with some mutations in the dnaK or grpE gene, are nonmotile, lack flagella, exhibit a 10- to 20-fold decrease in the rate of synthesis of flagellin, and show reduced rates of transcription of both the flhD master operon (encoding FlhD and FlhC) and the fliA operon (encoding sigma F). Genetic studies suggest that DnaK and DnaJ define a regulatory pathway affecting flhD and fliA synthesis that is independent of cyclic AMP-catabolite gene activator protein or the chemotaxis system.

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“…을 하는 박테리아에게는 매우 중요한 요인이다 [10,19,26,29]. 8.55 mM NaCl, 9.35 mM NH4Cl) 액체배지 또는 1.5% (w/v) agar를 첨가한 고체배지를 사용하여 37℃에서 배 양하였다 [3].…”

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Characterization and Identification of an Agar-Degrading Motile Bacteria Strain

Kang¹,

Yoo²,

Yu³

et al. 2012

Journal of Life Science

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A bacterial strain, CK214, exhibiting high motility on an LB agar (1.5%, w/v) surface was isolated from the environment. The formation of unusual agar shrinking around colonies on agar plates was observed. The strain grew on minimal media containing pure agar as a sole carbon source. The cell-free culture supernatant of CK214 generated a reduced form of sugar in the in vitro reaction with the use of pure agar as a substrate, suggesting the secretion of an agar-degrading enzyme. The CK214 strain showed swarming motility on the solid media containing a wide range of concentrations of agar (0.5, 1.0, 1.5, 2.0% w/v). Various tests, including Gram staining, API analysis, and phylogenetic analysis based on 16S rDNA sequences identified that the CK214 strain was a G(+) rod-shaped bacterium grouped in genus Paenibacillus. Electron microscopic analysis demonstrated that the P. CK214 strain is peritrichously flagellated. Through transposon random mutagenesis, several agar-degrading activity defective mutants (ADMs) were generated. These mutants will be used in the future experimentation for the study of the correlation between agar-degrading activity and motility.

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Bacterial chemotaxis controls the catabolite repression of flagellar biogenesis

Cited by 7 publications

References 13 publications

Functional interaction between information processing pathways in Escherichia coli and the nature of Δ-H+ (redox)-sensing

Functional interaction between information processing pathways in Escherichia coli and the nature of Δ-H+ (redox)-sensing

DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli

Characterization and Identification of an Agar-Degrading Motile Bacteria Strain

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